pMCS-Green-Renilla-Luc

pMCS-Green-Renilla-Luc

载体基本信息

出品公司:	Ybscience
载体名称:	pMCS-Green-Renilla-Luc 或 pMCS-Green Renilla Luc 、 pMCS-Renilla Luc
质粒类型:	哺乳动物表达载体；绿色荧光素酶报告载体
高拷贝/低拷贝:	高拷贝
克隆方法:	限制性内切酶，多克隆位点
启动子:	无
载体大小:	5263 bp
5' 测序引物及序列:	--
3' 测序引物及序列:	--
载体标签:	--
载体抗性:	氨苄青霉素
筛选标记:	嘌呤霉素（Puromycin）
克隆菌株:	DH5α 等
宿主细胞（系）:	常规细胞系（293、CV-1、CHO等）
备注:	--
稳定性:	稳表达
组成型/诱导型:	组成型
病毒/非病毒:	非病毒

 

载体质粒图谱和多克隆位点信息

 

载体简介

 

pMCS-Green Renilla Luc 载体简介

The Thermo Scientific pMCS-Renilla Luc vector is a multiple cloning site plasmid to designed to accept a promoter sequence for study of gene regulation using the intracellular Renilla luciferase reporter.

pMCS-Renilla Luc 载体特征:

 Intracellular Green Renilla luciferase gene, optimized for high expression in mammalian systems
 Multiple cloning site (MCS) provides versatility for transfer of regulatory elements from one plasmid to another
 Transcription termination site (Ter), Lac operator (Lac O1), and transcriptional pause site (TPS) used to minimize background by reducing transcriptional read-through
 Both puromycin (Pur) and ampicillin (Amp) markers for drug selection in mammalian and bacterial cells, respectively
 High-copy pUC bacterial DNA replication origin

The Green Renilla Luc Vectors contain a gene cloned from the sea pansy, Renilla reniformis. The gene encodes a bioluminescent Green Renilla luciferase (36kDa). The pMCS vector contains a multiple cloning site for cloning a promoter to study its regulatory potential.

These vectors are subject to a limited use label license.

 

载体序列

LOCUS       Exported                5263 bp ds-DNA     circular SYN 30-JUL-2016
DEFINITION  Intracellular green Renilla luciferase vector for cloning a 
            transcriptional regulatory element.
ACCESSION   .
VERSION     .
KEYWORDS    pMCS-Green Renilla Luc
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5263)
  AUTHORS   Thermo Scientific (Pierce)
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..5263
                     /organism="synthetic DNA construct"
                     /lab_host="Mammalian Cells"
                     /mol_type="other DNA"
     misc_feature    1..42
                     /note="MCS"
                     /note="multiple cloning site"
     CDS             49..984
                     /codon_start=1
                     /product="green-emitting variant of Renilla luciferase"
                     /note="Renilla Luciferase"
                     /note="human codon-optimized"
                     /translation="MASKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAEN
                     AVIFLHGNATSSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAW
                     FELLNLPKKIIFVGHDWGSALAFHYAYEHQDRIKAIVHMESVVDVIESWMGWPDIEEEL
                     ALIKSEEGEKMVLENNFFVETLLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPRE
                     IPLVKGGKPDVVQIVRNYNAYLRASDDLPKLFIESDPGFFSNAIVEGAKKFPNTEFVKV
                     KGLHFLQEDAPDEMGKYIKSFVERVLKNEQ"
     polyA_signal    1007..1118
                     /note="bGH poly(A) signal"
                     /note="bovine growth hormone polyadenylation signal"
     promoter        1241..1570
                     /note="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     rep_origin      1421..1556
                     /note="SV40 ori"
                     /note="SV40 origin of replication"
     promoter        1618..1665
                     /note="EM7 promoter"
                     /note="synthetic bacterial promoter "
     CDS             1684..2283
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /note="PuroR"
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     polyA_signal    2413..2534
                     /note="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     CDS             complement(2587..3447)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      3711..4299
                     /direction=RIGHT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    5041..5057
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="lac operator"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     misc_feature    5172..5263
                     /note="pause site"
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
ORIGIN
        1 actagtgaat tcggtaccga gctcctcgag aagcttggat ccgccaccat ggccagcaag
       61 gtgtacgacc ccgagcagcg gaagcggatg atcaccggcc ctcagtggtg ggctcggtgc
      121 aagcagatga acgtgctgga cagcttcatc aactactacg acagcgagaa gcacgccgag
      181 aacgccgtga tcttcctgca cggcaacgcc accagcagct acctgtggcg gcacgtggtg
      241 ccccacatcg agcctgtggc cagatgcatc atccccgacc tgatcggcat gggcaagagc
      301 ggcaagtccg gcaacggcag ctaccggctg ctggaccact acaagtacct gaccgcttgg
      361 tttgagctgc tgaacctgcc caagaagatc atcttcgtcg gccacgactg gggcagcgcc
      421 ctggcctttc actacgccta cgagcaccag gaccggatca aggccatcgt gcacatggaa
      481 agcgtggtgg acgtgatcga gagctggatg ggctggcccg acatcgagga agaactggcc
      541 ctgatcaaga gcgaagaggg cgagaagatg gtgctggaaa acaacttctt cgtggaaacc
      601 ctgctgccca gcaagatcat gcggaagctg gaacccgaag agttcgccgc ctacctggaa
      661 cccttcaaag aaaagggcga agtgcggagg cccaccctga gctggcccag agagatcccc
      721 ctggtcaagg gcggcaagcc cgacgtggtg cagatcgtgc ggaactacaa cgcctacctg
      781 cgggccagcg acgacctgcc taagctgttc atcgagagcg accccggctt cttcagcaac
      841 gccatcgtgg aaggcgccaa gaagttcccc aacaccgagt tcgtgaaagt gaagggcctg
      901 cacttcctcc aggaagatgc ccccgacgag atgggcaagt acatcaagag cttcgtggaa
      961 cgggtgctga agaacgagca gtgagcggcc gcaaaatcag cctcgactgt gccttctagt
     1021 tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact
     1081 cccactgtcc tttcctaata aaatgaggaa attgcatcac aacactcaac cctatctcgg
     1141 tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc
     1201 tgatttaaca aaaatttaac gcgaattaat tctgtggaat gtgtgtcagt tagggtgtgg
     1261 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
     1321 aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
     1381 caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc
     1441 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga
     1501 ggccgcctct gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg
     1561 cttttgcaaa aagctcccgg gagcttgtat atccattttc ggatctgatc agcacgtgtt
     1621 gacaattaat catcggcata gtatatcggc atagtataat acgacaaggt gaggaactaa
     1681 accatgaccg agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt ccccagggcc
     1741 gtacgcaccc tcgccgccgc gttcgccgac taccccgcca cgcgccacac cgtcgatccg
     1801 gaccgccaca tcgagcgggt caccgagctg caagaactct tcctcacgcg cgtcgggctc
     1861 gacatcggca aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg
     1921 gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc
     1981 ggttcccggc tggccgcgca gcaacagatg gaaggcctcc tggcgccgca ccggcccaag
     2041 gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg accaccaggg caagggtctg
     2101 ggcagcgccg tcgtgctccc cggagtggag gcggccgagc gcgccggggt gcccgccttc
     2161 ctggagacat ccgcgccccg caacctcccc ttctacgagc ggctcggctt caccgtcacc
     2221 gccgacgtcg aggtgcccga aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc
     2281 tgacacgtgc tacgagattt cgattccacc gccgccttct atgaaaggtt gggcttcgga
     2341 atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat gctggagttc
     2401 ttcgcccacc ccaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc
     2461 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc
     2521 atcaatgtat cttatcatgt ctgtataccg tcgacctcta gctagagctt ggcgtaatca
     2581 tggtcattac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
     2641 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
     2701 tggccccagc gctgcgatga taccgcgaga accacgctca ccggctccgg atttatcagc
     2761 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
     2821 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
     2881 gcgcaacgtt gttgccatcg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
     2941 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
     3001 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
     3061 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
     3121 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
     3181 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
     3241 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
     3301 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
     3361 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
     3421 ggcgacacgg aaatgttgaa tactcatatt cttccttttt caatattatt gaagcattta
     3481 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
     3541 aggggtcagt gttacaacca attaaccaat tctgaacatt atcgcgagcc catttatacc
     3601 tgaatatggc tcataacacc ccttgctcat gaccaaaatc ccttaacgtg agttacgcgc
     3661 gcgtcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
     3721 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
     3781 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     3841 cagataccaa atactgttct tctagtgtag ccgtagttag cccaccactt caagaactct
     3901 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
     3961 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     4021 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
     4081 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
     4141 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     4201 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
     4261 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
     4321 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
     4381 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga
     4441 acgaccgagc gcagcgagtc agtgagcgag gaagcggaag gcgagagtag ggaactgcca
     4501 ggcatcaaac taagcagaag gcccctgacg gatggccttt ttgcgtttct acaaactctt
     4561 tctgtgttgt aaaacgacgg ccagtcttaa gctcgggccc cctgggcggt tctgataacg
     4621 agtaatcgtt aatccgcaaa taacgtaaaa acccgcttcg gcgggttttt ttatgggggg
     4681 agtttaggga aagagcattt gtcagaatat ttaagggcgc ctgtcacttt gcttgatata
     4741 tgagaattat ttaaccttat aaatgagaaa aaagcaacgc actttaaata agatacgttg
     4801 ctttttcgat tgatgaacac ctataattaa actattcatc tattatttat gattttttgt
     4861 atatacaata tttctagttt gttaaagaga attaagaaaa taaatctcga aaataataaa
     4921 gggaaaatca gtttttgata tcaaaattat acatgtcaac gataatacaa aatataatac
     4981 aaactataag atgttatcag tatttattat catttagaat aaattttgtg tcgcccttaa
     5041 ttgtgagcgg ataacaatta cgagcttcat gcacagtggc gttgacattg attattgact
     5101 agcatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgccatgcat
     5161 tagttattaa taacatacgc tctccatcaa aacaaaacga aacaaaacaa actagcaaaa
     5221 taggctgtcc ccagtgcaag tgcaggtgcc agaacatttc tct