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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20℃
- 保质期:
6个月
- 英文名:
pFLD/CAT
- 库存:
大量
- 供应商:
钰博生物
- 规格:
1ug/5ug
载体基本信息
| 出品公司: | Ybscience |
|---|---|
| 载体名称: | pFLD/CAT |
| 质粒类型: | 酵母表达载体 |
| 高拷贝/低拷贝: | 高拷贝 |
| 启动子: | pFLD1 |
| 克隆方法: | 多克隆位点,限制性内切酶 |
| 载体大小: | 5.0 kb |
| 5' 测序引物及序列: | -- |
| 3' 测序引物及序列: | -- |
| 载体标签: | V5 epitope; 6XHis |
| 载体抗性: | Ampicillin |
| 筛选标记: | Zeocin |
| 备注: | 作为阳性对照。用于巴斯德毕赤酵母蛋白表达。诱导剂:甲胺 |
| 稳定性: | -- |
| 组成型: | 诱导型 |
| 病毒/非病毒: | 非病毒 |
载体质粒图谱和多克隆位点信息


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文献和实验A general protocol for staining cell for cytometry analysis
V-FITC (Cat. No. 65874X, 65874H) or Annexin V-Biotin (Cat. Nos. 65872X, 65872H). 3. Via-Probe™ (Cat. No. 34321X): A convenient ready-to-use solution of the nucleic acid dye 7-AAD. Recommended for use in parallel with Annexin V-PE (Cat
A general protocol for staining cell for cytometry analysis
. Store at RT. Recommended for use in parallel with Annexin V-FITC (Cat. No. 65874X, 65874H) or Annexin V-Biotin (Cat. Nos. 65872X, 65872H). 3.Via-Probe™ (Cat. No. 34321X): A convenient ready-to-use solution of the nucleic acid dye 7-AAD. Recommended
Protocol for Trichloroacetic Acid (TCA) Precipitation
and Equipment Required Borosilicate tubes, 12 x 75 mm (e.g., Fisher Cat #14-961-26) 10% TCA (e.g., Acros Cat # 42145-5000; make a 10% (w/v) solution in water) Warning: TCA is caustic! Carrier nucleic acid, 1 mg/ml (e.g., sheared fish
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