手机验证
-20℃
6个月
pTrcHis2 C
大量
钰博生物
1ug/5ug
出品公司: | Ybscience |
---|---|
别名: | pTrcHis2C、 pTrcHis2 C |
质粒类型: | 大肠杆菌表达载体 |
表达水平: | 高拷贝 |
启动子: | Trc |
克隆方法: | 多克隆位点,限制性内切酶 |
载体大小: | 4405 bp |
5' 测序引物及序列: | pTrcHis-F: gaggtatatattaatgtatcg |
3' 测序引物及序列: | pTrcHis-R:GATTTAATCTGTATCAGG |
载体标签: | C-His, C-Myc |
载体抗性: | Ampicillin |
备注: | Hosts: E.coli NM522, E.coli INValphaF'. Related vectors: pSE420. |
稳定性: | 瞬时表达 Transient |
组成型: | 组成型 Constitutive |
病毒/非病毒: | 非病毒 |
The pTrcHis2 plasmids are pBR322-derived expression vectors designed for efficient recombinant protein expression and purification in E. coli. High levels of expression are possible using the trc (trp-lac) promoter (Egon et al., 1983) and the rrnB anti-termination region (Li et al., 1984). The trc promoter contains the –35 region of the trp promoter together with the –10 region of the lac promoter (Brosius et al., 1985; Egon et al., 1983; Mulligan et al., 1985). To regulate expression, the gene encoding Lac repressor (lacIq) is provided in the pTrcHis2 vectors, allowing regulation of the trc promoter regardless of whether the host strain contains a gene encoding the Lac repressor.
Isopropyl-Beta-D-thiogalactopyranoside (IPTG) is used to induce expression of your gene. Translation is enhanced by the bacteriophage T7 gene 10 translation enhancer and a minicistron that provides highly efficient translational restart into the open reading frame of the multiple cloning site. DNA inserts are positioned downstream and in frame with the initiation ATG and a C-terminal fusion peptide. The C-terminal peptide encodes the myc epitope and six histidine residues that function as a metal binding site in the expressed protein.
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