pCas9-GFP

pCas9-GFP

载体基本信息

出品公司:	Ybscience
载体名称:	pCas9-GFP 或 pCas9_GFP
质粒类型:	CRISPR/Cas9载体；基因敲除载体；哺乳动物细胞载体；双顺反子载体
高拷贝/低拷贝:	高拷贝
插入位点:	限制性内切酶，多克隆位点
启动子:	CBh
载体大小:	9271 bp
5' 测序引物及序列:	ggctctagtgcctctgctaacc
3' 测序引物及序列:	M13 Reverse：CAGGAAACAGCTATGAC
载体标签:	T2A-GFP
载体抗性:	氨苄青霉素
筛选标记:	EGFP绿色荧光蛋白
克隆菌株:	Stbl3
宿主细胞（系）:	人胚胎干细胞
备注:	Co-expression of human codon-optimized Cas9 nuclease and GFP, plasmid optimized for expression in human pluripotent stem cells
稳定性:	瞬表达或稳表达
组成型/诱导型:	组成型
病毒/非病毒:	非病毒

载体质粒图谱和多克隆位点信息

 

载体简介

 

载体序列

LOCUS       pCas9_GFP	9271 bp 	DNA	SYN
DEFINITION  pCas9_GFP
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..9271
                     /organism="pCas9_GFP"
                     /mol_type="other DNA"
     misc_feature    88..375
                     /label="CAG_enhancer"
     CDS             259..738
                     /label="ORF frame 1"
                     /translation="MPSTPPIDVNDGKWPAWHYAQYMTLWDFPTWQYIYVLVIAITMG
                     RGEPHVLLHSPHLPPLPTPNFVFIYFLIILCSDGGGGGGGARQAGRGGARGGAGRGGE
                     VRRQPIRAARSESFLLWRGGGGGGPIKSEARGGRESLRCLRPVPRSAPPRAARPGSD*"
     CDS             complement(806..1351)
                     /label="ORF frame 1"
                     /translation="MAAARRASTAAGAPGAAAPLPRALPGPRRPRPARHPHLPPPPAR
                     HGEPRATPRTEPRTRSRAVLSNSGRGVQGGVTARPPRPHPLLTPPRTHPARSLCSPRS
                     PPAPRGTAPGRAPLAHTRSAQSPAPRPQRSQPPGSAGRTRRSPRTHTRTHPPSRSPPH
                     KGPSRSPLRLSRSHRKETSRH*"
     CDS             1490..6670
                     /label="ORF frame 2"
                     /translation="MGGEGLRASPRRRPLLHLQPRGCPQGDGCLRGGRGRAGFGFWRV
                     TGGSSASANHVHAFFFFLQLLGNVLVIVLSHHFGKEFAAATMDKKYSIGLDIGTNSVG
                     WAVITDEYKVPSKKFKVLGNTDRHSIKKNLIGALLFDSGETAEATRLKRTARRRYTRR
                     KNRICYLQEIFSNEMAKVDDSFFHRLEESFLVEEDKKHERHPIFGNIVDEVAYHEKYP
                     TIYHLRKKLVDSTDKADLRLIYLALAHMIKFRGHFLIEGDLNPDNSDVDKLFIQLVQT
                     YNQLFEENPINASGVDAKAILSARLSKSRRLENLIAQLPGEKKNGLFGNLIALSLGLT
                     PNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQIGDQYADLFLAAKNLSDAILLSDILR
                     VNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQQLPEKYKEIFFDQSKNGYAGYIDG
                     GASQEEFYKFIKPILEKMDGTEELLVKLNREDLLRKQRTFDNGSIPHQIHLGELHAIL
                     RRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGNSRFAWMTRKSEETITPWNFEEV
                     VDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYFTVYNELTKVKYVTEGMRKPAF
                     LSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDSVEISGVEDRFNASLGTYHDL
                     LKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERLKTYAHLFDDKVMKQLKRRR
                     YTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQLIHDDSLTFKEDIQKAQV
                     SGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRHKPENIVIEMARENQTTQ
                     KGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYLYYLQNGRDMYVDQELD
                     INRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPSEEVVKKMKNYWRQLL
                     NAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHVAQILDSRMNTKYDE
                     NDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLNAVVGTALIKKYPK
                     LESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTEITLANGEIRKRP
                     LIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKESILPKRNSDKL
                     IARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGITIMERSSFEKN
                     PIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNELALPSKYVNF
                     LYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADANLDKVLSA
                     YNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLDATLIHQS
                     ITGLYETRIDLSQLGGDSRADPKKKRKVRSGGGEGRGSLLTCGDVEENPGPMVSKGEE
                     LFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTL
                     TYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNR
                     IELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLAD
                     HYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK
                     *"
     CDS             complement(1673..2224)
                     /label="ORF frame 1"
                     /translation="MRQREIDQPQVSLISTVYKLLPQMIYGWVLFMVRHLVHDIAKDW
                     VALVLFILLHQKGLLQPMEERVIHLSHLITKDLLQVADPILSAGISAPCCSFEPRGFG
                     RLPGVEQEGANEVLLYAVAIGIAQNFEFFARHLVLVRNDGPADAVCADIEPNGVLLVH
                     GGGREFFAKMMRQHNNQHVAQEL*"
     misc_feature    1686..1705
                     /label="pCAG_F_primer"
                     /translation="MRQREIDQPQVSLISTVYKLLPQMIYGWVLFMVRHLVHDIAKDW
                     VALVLFILLHQKGLLQPMEERVIHLSHLITKDLLQVADPILSAGISAPCCSFEPRGFG
                     RLPGVEQEGANEVLLYAVAIGIAQNFEFFARHLVLVRNDGPADAVCADIEPNGVLLVH
                     GGGREFFAKMMRQHNNQHVAQEL*"
     CDS             complement(3149..3667)
                     /label="ORF frame 1"
                     /translation="MSVSFQAFFNHLPIFKQRKGEDNVLKNVLVLLIVQEVLVFNDFQ
                     EIVIRSQGCVEAILHSADFNRVETFNLFEIVFFELFHGNFPVRLEEEVHDSFLLLSRQ
                     ECWLSHPFCDVFDLGELVINCEVLVQQRVFRKHLFVRQIFIKVSHPFDEGLGRGPLIH
                     DFLEVPGSDGLF*"
     CDS             complement(3731..4264)
                     /label="ORF frame 1"
                     /translation="MIHVVVGEPIDVQFLIHVHVPAVLQVVQVELLILKLGVFNWVFL
                     KDLGPQFFYTLFNPLHPFPTVLLSLLGSLVLSGHLDNDILGLMPSHYFDEFIHDLNGL
                     QYSLFDSWATCKISDVLVKTVPLARNLCFLDVLLKGERVIMDQLHEVPVGKSIGLKKI
                     QDCLSTLLVSDPIDQFS*"
     CDS             complement(5258..5791)
                     /label="ORF frame 1"
                     /translation="MNQCGVQDLLCRGVPLSVYGGVEVLEGCRRAQVGQSKQVDNVFC
                     LLPDGLIPVLIVSRKHLIEVSVGEDHSFGEFAYLLDDLIKVVFVLFHEQLLLLIIFGR
                     PFELFIVAGQIQEINVFRGQCQLVTFLQLARTSEHSFPAVFKLKERVLGKLNDEVFFD
                     LFISFRLEKVDGVFFEA*"
     misc_feature    5861..5881
                     /label="SV40 NLS"
                     /translation="MNQCGVQDLLCRGVPLSVYGGVEVLEGCRRAQVGQSKQVDNVFC
                     LLPDGLIPVLIVSRKHLIEVSVGEDHSFGEFAYLLDDLIKVVFVLFHEQLLLLIIFGR
                     PFELFIVAGQIQEINVFRGQCQLVTFLQLARTSEHSFPAVFKLKERVLGKLNDEVFFD
                     LFISFRLEKVDGVFFEA*"
     misc_feature    5867..5881
                     /label="NLS"
                     /translation="MNQCGVQDLLCRGVPLSVYGGVEVLEGCRRAQVGQSKQVDNVFC
                     LLPDGLIPVLIVSRKHLIEVSVGEDHSFGEFAYLLDDLIKVVFVLFHEQLLLLIIFGR
                     PFELFIVAGQIQEINVFRGQCQLVTFLQLARTSEHSFPAVFKLKERVLGKLNDEVFFD
                     LFISFRLEKVDGVFFEA*"
     gene            5954..6667
                     /label="EGFP"
                     /gene="EGFP"
                     /translation="MNQCGVQDLLCRGVPLSVYGGVEVLEGCRRAQVGQSKQVDNVFC
                     LLPDGLIPVLIVSRKHLIEVSVGEDHSFGEFAYLLDDLIKVVFVLFHEQLLLLIIFGR
                     PFELFIVAGQIQEINVFRGQCQLVTFLQLARTSEHSFPAVFKLKERVLGKLNDEVFFD
                     LFISFRLEKVDGVFFEA*"
     misc_feature    complement(5996..6017)
                     /label="EGFP_N_primer"
                     /translation="MNQCGVQDLLCRGVPLSVYGGVEVLEGCRRAQVGQSKQVDNVFC
                     LLPDGLIPVLIVSRKHLIEVSVGEDHSFGEFAYLLDDLIKVVFVLFHEQLLLLIIFGR
                     PFELFIVAGQIQEINVFRGQCQLVTFLQLARTSEHSFPAVFKLKERVLGKLNDEVFFD
                     LFISFRLEKVDGVFFEA*"
     misc_feature    6134..6163
                     /label="Y66 (EGFP)"
                     /translation="MNQCGVQDLLCRGVPLSVYGGVEVLEGCRRAQVGQSKQVDNVFC
                     LLPDGLIPVLIVSRKHLIEVSVGEDHSFGEFAYLLDDLIKVVFVLFHEQLLLLIIFGR
                     PFELFIVAGQIQEINVFRGQCQLVTFLQLARTSEHSFPAVFKLKERVLGKLNDEVFFD
                     LFISFRLEKVDGVFFEA*"
     misc_feature    6604..6625
                     /label="EGFP_C_primer"
                     /translation="MNQCGVQDLLCRGVPLSVYGGVEVLEGCRRAQVGQSKQVDNVFC
                     LLPDGLIPVLIVSRKHLIEVSVGEDHSFGEFAYLLDDLIKVVFVLFHEQLLLLIIFGR
                     PFELFIVAGQIQEINVFRGQCQLVTFLQLARTSEHSFPAVFKLKERVLGKLNDEVFFD
                     LFISFRLEKVDGVFFEA*"
     terminator      6716..6835
                     /label="SV40_PA_terminator"
                     /translation="MNQCGVQDLLCRGVPLSVYGGVEVLEGCRRAQVGQSKQVDNVFC
                     LLPDGLIPVLIVSRKHLIEVSVGEDHSFGEFAYLLDDLIKVVFVLFHEQLLLLIIFGR
                     PFELFIVAGQIQEINVFRGQCQLVTFLQLARTSEHSFPAVFKLKERVLGKLNDEVFFD
                     LFISFRLEKVDGVFFEA*"
     misc_feature    6804..6823
                     /label="EBV_rev_primer"
                     /translation="MNQCGVQDLLCRGVPLSVYGGVEVLEGCRRAQVGQSKQVDNVFC
                     LLPDGLIPVLIVSRKHLIEVSVGEDHSFGEFAYLLDDLIKVVFVLFHEQLLLLIIFGR
                     PFELFIVAGQIQEINVFRGQCQLVTFLQLARTSEHSFPAVFKLKERVLGKLNDEVFFD
                     LFISFRLEKVDGVFFEA*"
     promoter        complement(6879..6897)
                     /label="M13_reverse_primer"
                     /translation="MNQCGVQDLLCRGVPLSVYGGVEVLEGCRRAQVGQSKQVDNVFC
                     LLPDGLIPVLIVSRKHLIEVSVGEDHSFGEFAYLLDDLIKVVFVLFHEQLLLLIIFGR
                     PFELFIVAGQIQEINVFRGQCQLVTFLQLARTSEHSFPAVFKLKERVLGKLNDEVFFD
                     LFISFRLEKVDGVFFEA*"
     misc_feature    complement(6896..6918)
                     /label="M13_pUC_rev_primer"
                     /translation="MNQCGVQDLLCRGVPLSVYGGVEVLEGCRRAQVGQSKQVDNVFC
                     LLPDGLIPVLIVSRKHLIEVSVGEDHSFGEFAYLLDDLIKVVFVLFHEQLLLLIIFGR
                     PFELFIVAGQIQEINVFRGQCQLVTFLQLARTSEHSFPAVFKLKERVLGKLNDEVFFD
                     LFISFRLEKVDGVFFEA*"
     promoter        complement(6932..6961)
                     /label="lac_promoter"
                     /translation="MNQCGVQDLLCRGVPLSVYGGVEVLEGCRRAQVGQSKQVDNVFC
                     LLPDGLIPVLIVSRKHLIEVSVGEDHSFGEFAYLLDDLIKVVFVLFHEQLLLLIIFGR
                     PFELFIVAGQIQEINVFRGQCQLVTFLQLARTSEHSFPAVFKLKERVLGKLNDEVFFD
                     LFISFRLEKVDGVFFEA*"
     gene            complement(8044..8904)
                     /label="Ampicillin"
                     /gene="Ampicillin"
                     /translation="MNQCGVQDLLCRGVPLSVYGGVEVLEGCRRAQVGQSKQVDNVFC
                     LLPDGLIPVLIVSRKHLIEVSVGEDHSFGEFAYLLDDLIKVVFVLFHEQLLLLIIFGR
                     PFELFIVAGQIQEINVFRGQCQLVTFLQLARTSEHSFPAVFKLKERVLGKLNDEVFFD
                     LFISFRLEKVDGVFFEA*"
     CDS             complement(8044..8904)
                     /label="ORF frame 2"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
     promoter        complement(8946..8974)
                     /label="AmpR_promoter"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
ORIGIN
    1 acgcgttgac attgattatt gactagttat taatagtaat caattacggg gtcattagtt
   61 catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga
  121 ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca
  181 atagggactt tccattgacg tcaatgggtg gactatttac ggtaaactgc ccacttggca
  241 gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg
  301 cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc
  361 tacgtattag tcatcgctat taccatgggt cgaggtgagc cccacgttct gcttcactct
  421 ccccatctcc cccccctccc cacccccaat tttgtattta tttatttttt aattattttg
  481 tgcagcgatg ggggcggggg gggggggggc gcgcgccagg cggggcgggg cggggcgagg
  541 ggcggggcgg ggcgaggcgg agaggtgcgg cggcagccaa tcagagcggc gcgctccgaa
  601 agtttccttt tatggcgagg cggcggcggc ggcggcccta taaaaagcga agcgcgcggc
  661 gggcgggagt cgctgcgttg ccttcgcccc gtgccccgct ccgcgccgcc tcgcgccgcc
  721 cgccccggct ctgactgacc gcgttactcc cacaggtgag cgggcgggac ggcccttctc
  781 ctccgggctg taattagcgc ttggtttaat gacggctcgt ttcttttctg tggctgcgtg
  841 aaagccttaa agggctccgg gagggccctt tgtgcggggg ggagcggctc ggggggtgcg
  901 tgcgtgtgtg tgtgcgtggg gagcgccgcg tgcggcccgc gctgcccggc ggctgtgagc
  961 gctgcgggcg cggcgcgggg ctttgtgcgc tccgcgtgtg cgcgagggga gcgcggccgg
 1021 gggcggtgcc ccgcggtgcg ggggggctgc gaggggaaca aaggctgcgt gcggggtgtg
 1081 tgcgtggggg ggtgagcagg gggtgtgggc gcggcggtcg ggctgtaacc cccccctgca
 1141 cccccctccc cgagttgctg agcacggccc ggcttcgggt gcggggctcc gtacggggcg
 1201 tggcgcgggg ctcgccgtgc cgggcggggg gtggcggcag gtgggggtgc cgggcggggc
 1261 ggggccgcct cgggccgggg agggctcggg ggaggggcgc ggcggccccc ggagcgccgg
 1321 cggctgtcga ggcgcggcga gccgcagcca ttgcctttta tggtaatcgt gcgagagggc
 1381 gcagggactt cctttgtccc aaatctgtgc ggagccgaaa tctgggaggc gccgccgcac
 1441 cccctctagc gggcgcgggg cgaagcggtg cggcgccggc aggaaggaaa tgggcgggga
 1501 gggccttcgt gcgtcgccgc gccgccgtcc ccttctccat ctccagcctc ggggctgtcc
 1561 gcagggggac ggctgccttc gggggggacg gggcagggcg gggttcggct tctggcgtgt
 1621 gaccggcggc tctagtgcct ctgctaacca tgttcatgcc ttcttctttt tcctacagct
 1681 cctgggcaac gtgctggtta ttgtgctgtc tcatcatttt ggcaaaGAAT TCGCGGCCGC
 1741 CACCATGGAC AAGAAGTACT CCATTGGGCT CGATATCGGC ACAAACAGCG TCGGCTGGGC
 1801 CGTCATTACG GACGAGTACA AGGTGCCGAG CAAAAAATTC AAAGTTCTGG GCAATACCGA
 1861 TCGCCACAGC ATAAAGAAGA ACCTCATTGG CGCCCTCCTG TTCGACTCCG GGGAGACGGC
 1921 CGAAGCCACG CGGCTCAAAA GAACAGCACG GCGCAGATAT ACCCGCAGAA AGAATCGGAT
 1981 CTGCTACCTG CAGGAGATCT TTAGTAATGA GATGGCTAAG GTGGATGACT CTTTCTTCCA
 2041 TAGGCTGGAG GAGTCCTTTT TGGTGGAGGA GGATAAAAAG CACGAGCGCC ACCCAATCTT
 2101 TGGCAATATC GTGGACGAGG TGGCGTACCA TGAAAAGTAC CCAACCATAT ATCATCTGAG
 2161 GAAGAAGCTT GTAGACAGTA CTGATAAGGC TGACTTGCGG TTGATCTATC TCGCGCTGGC
 2221 GCATATGATC AAATTTCGGG GACACTTCCT CATCGAGGGG GACCTGAACC CAGACAACAG
 2281 CGATGTCGAC AAACTCTTTA TCCAACTGGT TCAGACTTAC AATCAGCTTT TCGAAGAGAA
 2341 CCCGATCAAC GCATCCGGAG TTGACGCCAA AGCAATCCTG AGCGCTAGGC TGTCCAAATC
 2401 CCGGCGGCTC GAAAACCTCA TCGCACAGCT CCCTGGGGAG AAGAAGAACG GCCTGTTTGG
 2461 TAATCTTATC GCCCTGTCAC TCGGGCTGAC CCCCAACTTT AAATCTAACT TCGACCTGGC
 2521 CGAAGATGCC AAGCTTCAAC TGAGCAAAGA CACCTACGAT GATGATCTCG ACAATCTGCT
 2581 GGCCCAGATC GGCGACCAGT ACGCAGACCT TTTTTTGGCG GCAAAGAACC TGTCAGACGC
 2641 CATTCTGCTG AGTGATATTC TGCGAGTGAA CACGGAGATC ACCAAAGCTC CGCTGAGCGC
 2701 TAGTATGATC AAGCGCTATG ATGAGCACCA CCAAGACTTG ACTTTGCTGA AGGCCCTTGT
 2761 CAGACAGCAA CTGCCTGAGA AGTACAAGGA AATTTTCTTC GATCAGTCTA AAAATGGCTA
 2821 CGCCGGATAC ATTGACGGCG GAGCAAGCCA GGAGGAATTT TACAAATTTA TTAAGCCCAT
 2881 CTTGGAAAAA ATGGACGGCA CCGAGGAGCT GCTGGTAAAG CTTAACAGAG AAGATCTGTT
 2941 GCGCAAACAG CGCACTTTCG ACAATGGAAG CATCCCCCAC CAGATTCACC TGGGCGAACT
 3001 GCACGCTATC CTCAGGCGGC AAGAGGATTT CTACCCCTTT TTGAAAGATA ACAGGGAAAA
 3061 GATTGAGAAA ATCCTCACAT TTCGGATACC CTACTATGTA GGCCCCCTCG CCCGGGGAAA
 3121 TTCCAGATTC GCGTGGATGA CTCGCAAATC AGAAGAGACC ATCACTCCCT GGAACTTCGA
 3181 GGAAGTCGTG GATAAGGGGG CCTCTGCCCA GTCCTTCATC GAAAGGATGA CTAACTTTGA
 3241 TAAAAATCTG CCTAACGAAA AGGTGCTTCC TAAACACTCT CTGCTGTACG AGTACTTCAC
 3301 AGTTTATAAC GAGCTCACCA AGGTCAAATA CGTCACAGAA GGGATGAGAA AGCCAGCATT
 3361 CCTGTCTGGA GAGCAGAAGA AAGCTATCGT GGACCTCCTC TTCAAGACGA ACCGGAAAGT
 3421 TACCGTGAAA CAGCTCAAAG AAGACTATTT CAAAAAGATT GAATGTTTCG ACTCTGTTGA
 3481 AATCAGCGGA GTGGAGGATC GCTTCAACGC ATCCCTGGGA ACGTATCACG ATCTCCTGAA
 3541 AATCATTAAA GACAAGGACT TCCTGGACAA TGAGGAGAAC GAGGACATTC TTGAGGACAT
 3601 TGTCCTCACC CTTACGTTGT TTGAAGATAG GGAGATGATT GAAGAACGCT TGAAAACTTA
 3661 CGCTCATCTC TTCGACGACA AAGTCATGAA ACAGCTCAAG AGGCGCCGAT ATACAGGATG
 3721 GGGGCGGCTG TCAAGAAAAC TGATCAATGG GATCCGAGAC AAGCAGAGTG GAAAGACAAT
 3781 CCTGGATTTT CTTAAGTCCG ATGGATTTGC CAACCGGAAC TTCATGCAGT TGATCCATGA
 3841 TGACTCTCTC ACCTTTAAGG AGGACATCCA GAAAGCACAA GTTTCTGGCC AGGGGGACAG
 3901 TCTTCACGAG CACATCGCTA ATCTTGCAGG TAGCCCAGCT ATCAAAAAGG GAATACTGCA
 3961 GACCGTTAAG GTCGTGGATG AACTCGTCAA AGTAATGGGA AGGCATAAGC CCGAGAATAT
 4021 CGTTATCGAG ATGGCCCGAG AGAACCAAAC TACCCAGAAG GGACAGAAGA ACAGTAGGGA
 4081 AAGGATGAAG AGGATTGAAG AGGGTATAAA AGAACTGGGG TCCCAAATCC TTAAGGAACA
 4141 CCCAGTTGAA AACACCCAGC TTCAGAATGA GAAGCTCTAC CTGTACTACC TGCAGAACGG
 4201 CAGGGACATG TACGTGGATC AGGAACTGGA CATCAATCGG CTCTCCGACT ACGACGTGGA
 4261 TCATATCGTG CCCCAGTCTT TTCTCAAAGA TGATTCTATT GATAATAAAG TGTTGACAAG
 4321 ATCCGATAAA AATAGAGGGA AGAGTGATAA CGTCCCCTCA GAAGAAGTTG TCAAGAAAAT
 4381 GAAAAATTAT TGGCGGCAGC TGCTGAACGC CAAACTGATC ACACAACGGA AGTTCGATAA
 4441 TCTGACTAAG GCTGAACGAG GTGGCCTGTC TGAGTTGGAT AAAGCCGGCT TCATCAAAAG
 4501 GCAGCTTGTT GAGACACGCC AGATCACCAA GCACGTGGCC CAAATTCTCG ATTCACGCAT
 4561 GAACACCAAG TACGATGAAA ATGACAAACT GATTCGAGAG GTGAAAGTTA TTACTCTGAA
 4621 GTCTAAGCTG GTCTCAGATT TCAGAAAGGA CTTTCAGTTT TATAAGGTGA GAGAGATCAA
 4681 CAATTACCAC CATGCGCATG ATGCCTACCT GAATGCAGTG GTAGGCACTG CACTTATCAA
 4741 AAAATATCCC AAGCTTGAAT CTGAATTTGT TTACGGAGAC TATAAAGTGT ACGATGTTAG
 4801 GAAAATGATC GCAAAGTCTG AGCAGGAAAT AGGCAAGGCC ACCGCTAAGT ACTTCTTTTA
 4861 CAGCAATATT ATGAATTTTT TCAAGACCGA GATTACACTG GCCAATGGAG AGATTCGGAA
 4921 GCGACCACTT ATCGAAACAA ACGGAGAAAC AGGAGAAATC GTGTGGGACA AGGGTAGGGA
 4981 TTTCGCGACA GTCCGGAAGG TCCTGTCCAT GCCGCAGGTG AACATCGTTA AAAAGACCGA
 5041 AGTACAGACC GGAGGCTTCT CCAAGGAAAG TATCCTCCCG AAAAGGAACA GCGACAAGCT
 5101 GATCGCACGC AAAAAAGATT GGGACCCCAA GAAATACGGC GGATTCGATT CTCCTACAGT
 5161 CGCTTACAGT GTACTGGTTG TGGCCAAAGT GGAGAAAGGG AAGTCTAAAA AACTCAAAAG
 5221 CGTCAAGGAA CTGCTGGGCA TCACAATCAT GGAGCGATCA AGCTTCGAAA AAAACCCCAT
 5281 CGACTTTCTC GAGGCGAAAG GATATAAAGA GGTCAAAAAA GACCTCATCA TTAAGCTTCC
 5341 CAAGTACTCT CTCTTTGAGC TTGAAAACGG CCGGAAACGA ATGCTCGCTA GTGCGGGCGA
 5401 GCTGCAGAAA GGTAACGAGC TGGCACTGCC CTCTAAATAC GTTAATTTCT TGTATCTGGC
 5461 CAGCCACTAT GAAAAGCTCA AAGGGTCTCC CGAAGATAAT GAGCAGAAGC AGCTGTTCGT
 5521 GGAACAACAC AAACACTACC TTGATGAGAT CATCGAGCAA ATAAGCGAAT TCTCCAAAAG
 5581 AGTGATCCTC GCCGACGCTA ACCTCGATAA GGTGCTTTCT GCTTACAATA AGCACAGGGA
 5641 TAAGCCCATC AGGGAGCAGG CAGAAAACAT TATCCACTTG TTTACTCTGA CCAACTTGGG
 5701 CGCGCCTGCA GCCTTCAAGT ACTTCGACAC CACCATAGAC AGAAAGCGGT ACACCTCTAC
 5761 AAAGGAGGTC CTGGACGCCA CACTGATTCA TCAGTCAATT ACGGGGCTCT ATGAAACAAG
 5821 AATCGACCTC TCTCAGCTCG GTGGAGACAG CAGGGCTGAC CCCAAGAAGA AGAGGAAGGT
 5881 GAGGTCCGGC GGCGGAGAGG GCAGAGGAAG TCTTCTAACA TGCGGTGACG TGGAGGAGAA
 5941 TCCCGGCCCA ATGGTGAGCA AGGGCGAGGA GCTGTTCACC GGGGTGGTGC CCATCCTGGT
 6001 CGAGCTGGAC GGCGACGTAA ACGGCCACAA GTTCAGCGTG TCCGGCGAGG GCGAGGGCGA
 6061 TGCCACCTAC GGCAAGCTGA CCCTGAAGTT CATCTGCACC ACCGGCAAGC TGCCCGTGCC
 6121 CTGGCCCACC CTCGTGACCA CCCTGACCTA CGGCGTGCAG TGCTTCAGCC GCTACCCCGA
 6181 CCACATGAAG CAGCACGACT TCTTCAAGTC CGCCATGCCC GAAGGCTACG TCCAGGAGCG
 6241 CACCATCTTC TTCAAGGACG ACGGCAACTA CAAGACCCGC GCCGAGGTGA AGTTCGAGGG
 6301 CGACACCCTG GTGAACCGCA TCGAGCTGAA GGGCATCGAC TTCAAGGAGG ACGGCAACAT
 6361 CCTGGGGCAC AAGCTGGAGT ACAACTACAA CAGCCACAAC GTCTATATCA TGGCCGACAA
 6421 GCAGAAGAAC GGCATCAAGG TGAACTTCAA GATCCGCCAC AACATCGAGG ACGGCAGCGT
 6481 GCAGCTCGCC GACCACTACC AGCAGAACAC CCCCATCGGC GACGGCCCCG TGCTGCTGCC
 6541 CGACAACCAC TACCTGAGCA CCCAGTCCGC CCTGAGCAAA GACCCCAACG AGAAGCGCGA
 6601 TCACATGGTC CTGCTGGAGT TCGTGACCGC CGCCGGGATC ACTCTCGGCA TGGACGAGCT
 6661 GTACAAGTAA ctgcagcgcg gggatctcat gctggagttc ttcgcccacc ccaacttgtt
 6721 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc
 6781 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt
 6841 ctgtataccg tcgacctcta gctagagctt ggcgtaatca tggtcatagc tgtttcctgt
 6901 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa
 6961 agcctagggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc
 7021 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag
 7081 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
 7141 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
 7201 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
 7261 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
 7321 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
 7381 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
 7441 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
 7501 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
 7561 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
 7621 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
 7681 tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat
 7741 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
 7801 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
 7861 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
 7921 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
 7981 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
 8041 cagttaCCAA TGCTTAATCA GTGAGGCACC TATCTCAGCG ATCTGTCTAT TTCGTTCATC
 8101 CATAGTTGCC TGACTCCCCG TCGTGTAGAT AACTACGATA CGGGAGGGCT TACCATCTGG
 8161 CCCCAGTGCT GCAATGATAC CGCGAGACCC ACGCTCACCG GCTCCAGATT TATCAGCAAT
 8221 AAACCAGCCA GCCGGAAGGG CCGAGCGCAG AAGTGGTCCT GCAACTTTAT CCGCCTCCAT
 8281 CCAGTCTATT AATTGTTGCC GGGAAGCTAG AGTAAGTAGT TCGCCAGTTA ATAGTTTGCG
 8341 CAACGTTGTT GCCATTGCTA CAGGCATCGT GGTGTCACGC TCGTCGTTTG GTATGGCTTC
 8401 ATTCAGCTCC GGTTCCCAAC GATCAAGGCG AGTTACATGA TCCCCCATGT TGTGCAAAAA
 8461 AGCGGTTAGC TCCTTCGGTC CTCCGATCGT TGTCAGAAGT AAGTTGGCCG CAGTGTTATC
 8521 ACTCATGGTT ATGGCAGCAC TGCATAATTC TCTTACTGTC ATGCCATCCG TAAGATGCTT
 8581 TTCTGTGACT GGTGAGTACT CAACCAAGTC ATTCTGAGAA TAGTGTATGC GGCGACCGAG
 8641 TTGCTCTTGC CCGGCGTCAA TACGGGATAA TACCGCGCCA CATAGCAGAA CTTTAAAAGT
 8701 GCTCATcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
 8761 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
 8821 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
 8881 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
 8941 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
 9001 ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc gacggatcgg gagatcgatc
 9061 tcccgatccc ctagggtcga ctctcagtac aatctgctct gatgccgcat agttaagcca
 9121 gtatctgctc cctgcttgtg tgttggaggt cgctgagtag tgcgcgagca aaatttaagc
 9181 tacaacaagg caaggcttga ccgacaattg catgaagaat ctgcttaggg ttaggcgttt
 9241 tgcgctgctt cgcgatgtac gggccagata t