Recombinant Human ICOS Ligand / B7-H2 / ICOSLG Protein (ECD,His Tag) | 重组人 ICOS Ligand / B7-H2 / ICOSLG 蛋白 (ECD, His标签)
- ￥1550 - 10080
- Sino Biological
- 北京
- 11559-H08H
- 2025年08月12日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
在-20℃ to -80℃
- 保质期：
12个月
- 英文名：
Recombinant Human ICOS Ligand / B7-H2 / ICOSLG Protein (ECD,His Tag)
- 库存：
99
- 供应商：
北京义翘神州科技股份有限公司
- 规格：
100 µg/1 mg
| 规格： | 100 µg | 产品价格： | ￥1550.0 |
|---|---|---|---|
| 规格： | 1 mg | 产品价格： | ￥10080.0 |
蛋白名称：Human ICOS Ligand / B7-H2 / ICOSLG Protein (ECD,His Tag)
蛋白构建：A DNA sequence encoding the human ICOSLG (NP_056074.1) extracellular domain (Met 1-Ser 258) was fused with a polyhistidine tag at the C-terminus.
表达宿主：HEK293 Cells
蛋白纯度：> 98 % as determined by SDS-PAGE
蛋白活性：Measured by its binding ability in a functional ELISA . Immobilized human B7-H2 at 1 μg/ml (100 μl/well) can bind human ICOS with a linear range of 1.6-200 ng/ml.
蛋白内毒素：< 1.0 EU per μg of the protein as determined by the LAL method
预测N端：Asp 19
蛋白分子量：The secreted recombinant human ICOSLG consists of 251 amino acids and has a calculated molecular mass of 28 kDa. The apparent molecular mass of the protein is approximately 42-62 kDa in SDS-PAGE under reducing conditions due to glycosylation.
蛋白NP号：NP_056074.1
蛋白氨基酸序列：Met1-Ser258
蛋白标签：C-His
蛋白保存条件：Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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文献和实验Small scale His-Tag fusion protein purification under denaturative conditions
Small scale His-Tag fusion protein purification under denaturative conditions Introduction High levels of expression of recombinant proteins in a bacterial system can lead to the formation of insoluble aggregates
Ligand Characterization Using Microphysiometry
., and Pitchford, S. 1994. Recombinant human ciliary neurotrophic factor stimulates metabolic activity of SH‐SY5Y cells as measured by a Cytosensor microphysiometer. Brain Res. 646:327‐331.
Small Scale GST-Tag Purification Under Nature Conditions
of overloading). 3) Wash beads with 2x1.5ml PBS (washing: mix, spin 3min 3500rpm, keep supernatant aside). Keep sample of 40ul for PAGE-SDS of each washing . 4) Elute recombinant protein with 3 x 50ul elution buffer: 50mM TrisHCl pH8.0 10mM reduce
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