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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 保存条件:
-20℃ to -80℃
- 保质期:
12个月
- 英文名:
Recombinant Human Fibronectin / Fibronectin Fragment 2 Protein (His Tag)
- 库存:
99
- 供应商:
北京义翘神州科技股份有限公司
- 规格:
1.00 mg/100.00 µg/200.00 µg
| 规格: | 1.00 mg | 产品价格: | ¥12580.0 |
|---|---|---|---|
| 规格: | 100.00 µg | 产品价格: | ¥1920.0 |
| 规格: | 200.00 µg | 产品价格: | ¥2570.0 |
蛋白名称:Human Fibronectin / Fibronectin Fragment 2 Protein (His Tag)
蛋白构建:A DNA sequence encoding the Fragment 2 (Ser607-Pro1265) of human Fibronectin (NP_997647.1) was expressed with a C-terminal polyhistidine tag.
表达宿主:HEK293 Cells
蛋白纯度:> 97 % as determined by SDS-PAGE.
蛋白活性:Measured by the ability of the immobilized protein to support the adhesion of NIH-3T3 mouse embryonic fibroblast cells. When cells are added to Fibronectin coated plates (2.5 μg/mL and 100 μL/well), approximately 35%-60% cells will adhere specifically after 35 minutes at 37℃.
蛋白内毒素:< 1.0 EU per μg of the protein as determined by the LAL method
预测N端:Ser 607
蛋白分子量:The secreted recombinant human Fibronectin 1 fragment2 (FN1.2) consists of 670 amino acids and has a calculated molecular mass of 73.2 kDa. As a result of glycosylation, rhFN1.2 migrates as an approximately 94.1 kDa band in SDS-PAGE under reducing conditions.
蛋白NP号:NP_997647.1
蛋白氨基酸序列:Ser607-Pro1265
蛋白标签:C-His
蛋白保存条件:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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- 作者
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文献和实验Preparation of recombinant fibronectin fragments for functional and structural studies
integrin receptors. The mosaic nature of fibronectin permits it to be analyzed by a “dissection” strategy, where protein fragments generated by recombinant expression in E. coli , P. pastoris , and human cell lines are employed in structural and functional
Efficient Retroviral Gene Transfer to Epidermal Stem Cells
. Here we describe an efficient protocol that results in high levels of retroviral gene transfer to human epidermal stem cells by immobilizing retrovirus on a recombinant fibronectin (rFN) fragment. In contrast to the traditional method, transduction on rFN promotes gene transfer
GPI蛋白 本文提供了有关糖基磷脂酰肌醇(GPI,是许多蛋白以及病毒蛋白结合于膜上的锚点)的信息。同时,网站还提供了许多相关链接和资源。 GPI overview GPI proteins in chickens GPI proteins in Drosophila GPI in prion proteins Human GPI proteins: protectin (CD59) and homologous restriction factor (HRF
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