重组人 TNFR1/CD120a 蛋白

重组人 TNFR1 / CD120a / TNFRSF1A 蛋白 (His标签)（产品说明）
蛋白名称：Human TNFR1 / CD120a / TNFRSF1A Protein (His Tag)
蛋白构建：A DNA sequence encoding the human TNFRI (NP_001056.1) extracellular domain (Met 1-Thr 211) was fused with a polyhistidine tag at the C-terminus.
表达宿主：HEK293 Cells
蛋白纯度：> 92 % as determined by SDS-PAGE
蛋白活性：1. Measured by its binding ability in a functional ELISA. Immobilized human TNFRI-His at 10 μg/mL (100 μl/well) can bind biotinylated human TNFα. The EC50 of biotinylated human TNFα is 5-12 μg/mL. 2. Measured by its ability to inhibit TNFα-mediated cytotoxicity in L929 mouse fibrosarcoma cells in the presence of metabolic inhibitor actinomycin D. The ED50 for this effect is typically 0.2-1μg/mL in the presence of 0.25 ng/mL recombinant human TNFα.
蛋白内毒素：< 1.0 EU per μg of the protein as determined by the LAL method
预测N端：Ile 22
蛋白分子量：The recombinant human TNFRI consists of 201 amino acids after removal of the signal peptide and has a predicted molecular mass of 22.7 kDa. As a result of glycosylation, the apparent molecular mass of rh TNFRI is 30-35 kDa in SDS-PAGE under reducing conditions.
蛋白NP号：NP_001056.1
蛋白氨基酸序列：Met1-Thr211
蛋白标签：C-His
蛋白保存条件：Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.