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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
在-20℃ to -80℃
- 保质期:
12个月
- 英文名:
Recombinant Mouse Acetylcholinesterase / ACHE Protein (His Tag)
- 库存:
99
- 供应商:
北京义翘神州科技股份有限公司
- 规格:
20 µg
蛋白名称:Acetylcholinesterase蛋白, Acetylcholinesterase protein
蛋白构建:A DNA sequence encoding the mouse ACHE (NP_033729.1) (Met 1-Leu 614) was expressed, with a polyhistidine tag at the C-terminus.
表达宿主:HEK293 Cells
蛋白纯度:> 97 % as determined by SDS-PAGE
蛋白活性:Measured by its ability to cleave Acetylthiocholine. The specific activity is > 250 nmols/min/μg.
蛋白内毒素:< 1.0 EU per μg of the protein as determined by the LAL method
预测N端:Glu 32
蛋白分子量:The recombinant mouse ACHE consists of 594 amino acids and has a predicted molecular mass of 66.2 kDa as estimated in SDS-PAGE under reducing conditions.
蛋白NP号:NP_033729.1
蛋白氨基酸序列:Met1-Leu614
蛋白标签:C-His
蛋白保存条件:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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文献和实验Nucleic Acid Programmable Protein Arrays: Versatile Tools for Array‐Based Functional Protein Studies
‐affinity tag antibodies for development and determination of protein display, 2624B (Cell Signaling Technology) Hybridization chamber (Corning, product no. 2551
【资源】全面归类网址:毕赤酵母表达各种来源蛋白(细菌,真菌,植物,人类等)
α-amylaseS, 240 mg/L ,activeMouse sonic hedgehog proteinS, α-MF, 500 mg/L, active392Mouse major urinary protein complex (MUP)S, 270 mg/L, native[165]Recombinant non-hydroxlated gelatins(based on rat and mouse collagen)S, 14.8 g/L, α-MF385Ostrich egg
。[37] 使用这种方法,可以检测活小鼠胃肠道内重组的发光沙门氏菌的定殖和不同的喂食物质对其的影响。[38] 肿瘤研究 与药物功效研究相同,肿瘤的生长与转移,可以通过活体中注射BL重组肿瘤细胞,再成像进行监测。[39] 另一种方式,初期的肿瘤和不确定的转移可以通过使用基因工程的发光细胞作为探针进行定位。[40] a human colon carcinoma xenograft 模式中已经开始使用双标记,一个标记检测特定基因的转录活性,另一个组成性表达,作为一个内参照物。[41] 细胞
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