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- 文献和实验
- 技术资料
- 保存条件:
-20℃ to -80℃
- 保质期:
12个月
- 英文名:
Recombinant Mouse RANKL / OPGL / TNFSF11 / CD254 Protein (Fc Tag)
- 库存:
99
- 供应商:
北京义翘神州科技股份有限公司
- 规格:
10 µg/20 µg/50 µg
| 规格: | 10 µg | 产品价格: | ¥1380.0 |
|---|---|---|---|
| 规格: | 20 µg | 产品价格: | ¥2480.0 |
| 规格: | 50 µg | 产品价格: | ¥2480.0 |
蛋白名称:Mouse RANKL / OPGL / TNFSF11 / CD254 Protein (Fc Tag)
蛋白构建:A DNA sequence encoding the extracellular domain of mouse TNFSF11 (AAC40113.1) (Arg 72-Asp 316) was fused with the Fc region of human IgG1 at the N-terminus.
表达宿主:HEK293 Cells
蛋白纯度:> 87 % as determined by SDS-PAGE
蛋白活性:Immobilized mouse Fc-TNFSF11 at 10 μg/ml (100 μl/well) can bind biotinylated human TNFRSF11B-His (Cat:10271-H08H), The EC50 of biotinylated human TNFRSF11B-His (Cat:10271-H08H) is 0.07-0.17 μg/ml.
蛋白内毒素:< 1.0 EU per μg of the protein as determined by the LAL method
预测N端:Glu
蛋白分子量:The recombinant mouse TNFSF11/Fc is a disulfide-linked homodimer. The reduced monomer comprises 505 amino acids and has a calculated molecular mass of 56 kDa. The apparent molecular mass of the monomer is approximately 50 kDa in SDS-PAGE under reducing conditions.
蛋白NP号:AAC40113.1
蛋白氨基酸序列:Arg72-Asp316
蛋白标签:N-human IgG1-Fc
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文献和实验There is an increasing interest in the preparation of rat � rat hybridomas, because they have been found to be more stable in culture than mouse hybridomas and they secrete consistently high levels (10 �g/mL and above) of monoclonal antibody
Notes on Making Rat x Y3 Monoclonal Antibody Producing Hybridomas
Although Y3/Ag1.2.3 has its own myeloma light chain, their are numerous characteristics that make this a better parent line for making hybridomas than the 'Y0' chain loss variants or using NS0 to make rat x mouse hybrids. These are: a) Hybridomas
Using the scan‐x Web Site to Predict Protein Post‐Translational Modifications
search page. This screenshot of the scan‐x main parameters/search page shows the appropriate parameters for the mouse Rb1 example provided in the . An in‐depth description of the various parameters can be found in the main text
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