CTLA-4重组蛋白|Recombinant Mouse CTLA4 / CD152 Protein (ECD,His Tag)

小鼠 CTLA-4蛋白|CTLA-4 protein|CTLA-4(Mouse, C-His)（产品说明）
蛋白名称：CTLA-4蛋白, CTLA-4 protein
蛋白构建：A DNA sequence encoding the extracellular domain of mouse CTLA4 (NP_033973.2) (Met 1-Phe 162) was expressed, with a polyhistidine tag at the C-terminus.
表达宿主：HEK293 Cells
蛋白纯度：> 98 % as determined by SDS-PAGE
蛋白活性：1. Measured by its binding ability in a functional ELISA. 2. Immobilized recombinant mouse CTLA4-His (Cat:50503-M08H) at 10 μg/ml (100 μl/well) can mouse CD80-Fc (cat:50446-M02H) with a linear range of 0.78-62.5 ng/ml. 3. Immobilized recombinant mouse CTLA4-His (Cat:50503-M08H) at 10 μg/ml (100 μl/well) can human B7-1-Fc (Cat:10698-H03H) with a linear range of 0.78-125 ng/ml. 4. Measured by its ability to inhibit IL-2 secretion by stimulated Jurkat human acute T cell leukemia cells. The ED50 for this effect is 0.1-0.5μg/mL when stimulated with 1 μg/mL Recombinant Human B7-1/CD80.
蛋白内毒素：< 1.0 EU per μg of the protein as determined by the LAL method
预测N端：Glu 36
蛋白分子量：The recombinant mouse CTLA4 consists of 138 amino acids and has a predicted molecular mass of 15.3 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rm CTLA4 is approximately 25-30 kDa due to glycosylation.
蛋白NP号：NP_033973.2
蛋白氨基酸序列：Met1-Phe162
蛋白标签：C-His
蛋白保存条件：Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.