Recombinant Mouse CTLA4 / CD152 Protein (ECD, Fc Tag) | 重组小鼠 CTLA4 / CD152 蛋白 (ECD, Fc标签)
- ￥1660 - 10760
- Sino Biological
- 北京
- 50503-M02H
- 2025年08月12日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
在-20℃ to -80℃
- 保质期：
12个月
- 英文名：
Recombinant Mouse CTLA4 / CD152 Protein (ECD, Fc Tag)
- 库存：
99
- 供应商：
北京义翘神州科技股份有限公司
- 规格：
100 µg/1 mg
| 规格： | 100 µg | 产品价格： | ￥1660.0 |
|---|---|---|---|
| 规格： | 1 mg | 产品价格： | ￥10760.0 |
蛋白名称：CTLA-4蛋白, CTLA-4 protein
蛋白构建：A DNA sequence encoding the extracellular domain of mouse CTLA4 (NP_033973.2) (Met 1-Phe 162) was fused with the Fc region of human IgG1 at the C-terminus.
表达宿主：HEK293 Cells
蛋白纯度：> 95 % as determined by SDS-PAGE
蛋白活性：Measured by its ability to inhibit IL-2 secretion by stimulated Jurkat human acute T cell leukemia cells. The ED50 for this effect is 0.05-0.3μg/mL when stimulated with 1 μg/mL Recombinant Human B7-1/CD80.
蛋白内毒素：< 1.0 EU per μg of the protein as determined by the LAL method
预测N端：Glu 36
蛋白分子量：The secreted recombinant mouse CTLA4/Fc is a disulfide-linked homodimeric protein. The reduced monomer comprises 368 amino acids and has a predicted molecular mass of 41 kDa. As a result of glycosylation, the apparent molecular mass of rm CTLA4/Fc monomer is approximately 55 kDa in SDS-PAGE under reducing conditions.
蛋白NP号：NP_033973.2
蛋白氨基酸序列：Met1-Phe162
蛋白标签：C-human IgG1-Fc
蛋白保存条件：Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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文献和实验2, Jacobs, L; et al. DNA-based delivery of checkpoint inhibitors in muscle and tumor enables long-term responses with distinct exposure. Molecular Therapy
Preparation of Recombinant Protein Spotted Arrays for Proteome‐Wide Identification of Kinase Targets
., Janjua, H., Shastry, R., Ho, C.K., Wang, D., Wang, H., Jiang, M., Montelione, G.T., Stuart, D.I., Owens, R.J., Daenke, S., Schutz, A., Heinemann, U., Yokoyama, S., Bussow, K., and Gunsalus, K.C. 2008. Protein production and purification. Nat. Methods
strains. 1. Use C57BL/6 F1 mice to set up new breeding cages (see mouse breeding suggestions above). Most C57BL/6 F1 hybrids have good reproductive performance. We prefer (C57BL/6J X DBA/2J)F1 animals. Outbred CD-1 and ICR mice can be used
-8878.3. Takayama, H. et al. 2008. High-level expression, single-step immunoaffinity purification and characterization of human tetraspanin membrane protein CD81. PLoS ONE 3: e2314 (DOI: 10.1371/journal.pone.0002314).4. Zhong, M. and Molday, R.S. 2010
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