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- 技术资料
- 规格:
100 µg
蛋白名称:Endoglin/CD105蛋白, Endoglin/CD105 protein
蛋白构建:A DNA sequence encoding the mouse CD105 (NP_031958.2) extracellular domain (Met 1-Gly 580) was expressed, with a C-terminal polyhistidine tag.
表达宿主:HEK293 Cells
蛋白纯度:> 97 % as determined by SDS-PAGE
蛋白活性:1. Measured by its ability to inhibit BMP9-induced alkaline phosphatase production by MC3T3E1 mouse chondrogenic cells.The ED50 for this effect is typically 25-100 ng/mL in the presence of 2 ng/mL of recombiant human BMP9.
蛋白内毒素:< 1.0 EU per μg of the protein as determined by the LAL method
预测N端:Arg 27
蛋白分子量:The secreted recombinant mouse CD105 consists of 565 amino acids and has a calculated molecular mass of 61.2 kDa. As a result of glycosylation, the apparent molecular mass of the recombinant protein is approximately 65-70 kDa in SDS-PAGE under reducing conditions.
蛋白NP号:NP_031958.2
蛋白氨基酸序列:Met1-Gly580
蛋白标签:C-His
蛋白保存条件:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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文献和实验Purification of Adipose Tissue Mesenchymal Stem Cells and Differentiation Toward Hepatic-Like Cells
yet; therefore, it is still not obvious how to purify these heterogeneous fraction of cells. We postulate that one of the markers defining MSC provenance is CD105 (endoglin). Therefore, we have sorted CD105+ fraction of AT-MSCs, expanded them, and differentiated
Barry FP, et al. The monoclonal antibody SH-2, raised against human mesenchymal stem cells, recognizes an epitope on endoglin (CD105). Biochem.Biophys.Res
蛋白质纯化的原理和方法(Protein Purification Principles and Methods)
and Hydrophobic-Interactions •“Weak” interactions are important for structure and function (activity) of the protein ÆIn most cases the purification must be gentle! Before the purification… •Cultivation of bacteria •Cell disruption
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