IL-27 蛋白|IL-27 protein|IL-27(Mouse, His Tag)

小鼠 IL-27蛋白|IL-27 protein|IL-27(Mouse, C-His)（产品说明）
蛋白名称：IL-27蛋白, IL-27 protein
蛋白构建：A DNA sequence encoding the mouse IL27 complex composed of EBI3 (O35228) (Met1-Pro228) and IL27A (Q8K3I6) (Phe29-Ser234) linked by an polypeptide linker was fused with the polyhistidine tag at the C-terminus.
表达宿主：HEK293 Cells
蛋白纯度：> 85 % as determined by SDS-PAGE
蛋白活性：1. Measured by its binding ability in a functional ELISA. Immobilized mouse IL27-His at 10 μg/ml (100 μl/well) can bind human IL27RA-Fc (Cat:10489-H02H), The EC50 of human IL27RA-Fc (Cat:10489-H02H) is 0.26-0.62 μg/ml. 2. Measured in antiviral assay using HepG2 cells infected with vesicular stomatitisvirus (VSV). The ED50 for this effect is 2-10ng/mL.
蛋白内毒素：< 1.0 EU per μg of the protein as determined by the LAL method
预测N端：Tyr 19
蛋白分子量：The recombinant mouse IL27 comprises 442 amino acids and has a predicted molecular mass of 49.5 kDa. The apparent molecular mass of the protein is approximately 60 kDa in SDS-PAGE under reducing conditions due to glycosylation.
蛋白NP号：O35228 & Q8K3I6
蛋白氨基酸序列：Met1-Pro228 & Phe29-Ser234
蛋白标签：C-His
蛋白保存条件：Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.