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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
在-20℃ to -80℃
- 保质期:
12个月
- 英文名:
Recombinant Human CDC2 Kinase / CDK1 Protein (GST Tag)
- 库存:
99
- 供应商:
北京义翘神州科技股份有限公司
- 规格:
50 µg
蛋白名称:Human CDC2 Kinase / CDK1 Protein (GST Tag)
蛋白构建:A DNA sequence encoding the human CDC2 isoform 1 (NP_001777.1) (Met 1-Met 297) was fused with the GST tag at the N-terminus.
表达宿主:Baculovirus-Insect Cells
蛋白纯度:> 85 % as determined by SDS-PAGE
蛋白活性:No Kinase Activity
蛋白内毒素:< 1.0 EU per μg of the protein as determined by the LAL method
预测N端:Met
蛋白分子量:The recombinant human CDC2/GST chimera consists of 522 amino acids and predicts a molecular mass of 60 kDa. It migrates as an approximately 53 kDa band in SDS-PAGE under reducing conditions.
蛋白NP号:NP_001777.1
蛋白氨基酸序列:Met1-Met297
蛋白标签:N-GST
蛋白保存条件:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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文献和实验Assays for Cyclin-Dependent Kinase Inhibitors
several substrates including Rb. On the other hand, CDK2/cyclin A complex is active throughout S phase. For cells to progress to mitosis, CDK1 (CDC2) must be “activated,” and activated cdc2 phosphorylates substrates required for mitosis (3 ).
三句话读懂一篇 CNS:咖啡竟可降低心律失常风险;转入肥胖基
(冷冻电镜技术),打破蛋白纯化等技术瓶颈,首次解析了 ATP 结合状态下的抑制型 AMPK 复合物的高分辨率结构,从结构生物学角度阐述了 AMPK 的活性调节机制,提出了 AMPK 激活、失活、再激活的循环通路模型!图 3:来源 Science4. STM:间质细胞在肠道免疫中的作用炎症性肠病增加了结直肠肿瘤的患病风险。2021 年 7 月 21 日,上海交通大学 Bio-X 研究院李保界教授等团队在 Science Translational Medicine 杂志上发表研究
mutations at phenylalanine in a putative PP-1C (the catalytic subunit) binding site motif(K/R)(X)(I/V)XF was unable to rescue Hxk2p dephosphorylation in intact yeast or restore Hxk2p phosphatase activity. These results demonstrate that Reg1p targets PP-1C
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