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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
在-20℃ to -80℃
- 保质期:
12个月
- 英文名:
Recombinant Human CDC2 Kinase / CDK1 Protein (GST Tag)
- 库存:
99
- 供应商:
北京义翘神州科技股份有限公司
- 规格:
50 µg
蛋白名称:Human CDC2 Kinase / CDK1 Protein (GST Tag)
蛋白构建:A DNA sequence encoding the human CDC2 isoform 1 (NP_001777.1) (Met 1-Met 297) was fused with the GST tag at the N-terminus.
表达宿主:Baculovirus-Insect Cells
蛋白纯度:> 85 % as determined by SDS-PAGE
蛋白活性:No Kinase Activity
蛋白内毒素:< 1.0 EU per μg of the protein as determined by the LAL method
预测N端:Met
蛋白分子量:The recombinant human CDC2/GST chimera consists of 522 amino acids and predicts a molecular mass of 60 kDa. It migrates as an approximately 53 kDa band in SDS-PAGE under reducing conditions.
蛋白NP号:NP_001777.1
蛋白氨基酸序列:Met1-Met297
蛋白标签:N-GST
蛋白保存条件:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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文献和实验Preparation of Recombinant Protein Spotted Arrays for Proteome‐Wide Identification of Kinase Targets
kinase substrate identification. This approach enables the rapid determination of protein interaction networks in yeast on a proteome?wide level. The same methodology can be readily applied to higher eukaryotic systems with careful consideration
3B Microtubule-associated protein 2 (MAP2) (42) AAUCCUUCGUCUUUCCUCUCG primary neuronal cells Myc-induced nuclear antigen 53 (mina53) (40) 45-63 See reference HeLa, MycB MPS1 kinase (hMps1) (8) 133-155 See reference Human HeLa S3 Myeloid cell leukemia protein
Identifying Protein Interactions by Hydroxyl‐Radical Protein Footprinting
containing a protein kinase recognition motif, His6‐tag and hemagglutinin epitope. Gene 166:177‐178. Kim, K., Rhee, S.G., and Stadtman, E.R. 1985. Nonenzymatic cleavage of proteins
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