- ￥3870 - 8940
- Sino Biological
- 北京
- 10518-H49H-B
- 2025年08月12日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20℃ to -80℃
- 保质期：
12个月
- 英文名：
Recombinant Human IL-3R alpha/CD123 Protein (His & AVI Tag), Biotinylated
- 库存：
99
- 供应商：
北京义翘神州科技股份有限公司
- 规格：
20 µg/100 µg
| 规格： | 20 µg | 产品价格： | ￥3870.0 |
|---|---|---|---|
| 规格： | 100 µg | 产品价格： | ￥8940.0 |
蛋白名称：Human IL-3R alpha/CD123 Protein (His & AVI Tag), Biotinylated
蛋白构建：A DNA sequence encoding the Human IL3Ra (NP_002174.1) (Met1-Arg305) was expressed with a C-terminal polyhistidine tag followed by an AVI tag. The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed.
表达宿主：HEK293 Cells
蛋白纯度：≥ 95 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC.
蛋白活性：Loaded Recombinant Human IL-3R alpha/CD123 Protein, His Tag, Biotinylated (Cat. No. 10518-H49H-B) on SA Biosensor, can bind Recombinant Human IL3 Protein, no Tag (Cat. No. 11858-HNAE) with an affinity constant of 0.4µM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested).
蛋白内毒素：< 1.0 EU per μg protein as determined by the LAL method.
预测N端：Thr 19
蛋白分子量：The recombinant Human IL3Ra consists of 313 amino acids and predicts a molecular mass of 36.34 kDa. It migrates as an approximately 56.42 kDa band in SDS-PAGE under reducing conditions.
蛋白NP号：NP_002174.1
蛋白氨基酸序列：Met1-Arg305
蛋白标签：C-His-AVI
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文献和实验X-100或脱氧胆酸钠）和低浓度变性剂（2mol/L尿素或盐酸胍等）洗涤除去脂类和膜蛋白，这一步很重要，否则会导致包涵体溶解和复性的过程中重组蛋白质的降解[6、7、8]。 包涵体的溶解必须用很强的变性剂，如8mol/L尿素、6~8mol/L盐酸胍，通过离子间的相互作用破坏包涵体蛋白间的氢键而增溶蛋白。其中尿素的增溶效果稍差，异氰盐酸胍最强；去污剂，如SDS[7]，可以破坏蛋白内的疏水键，可以增溶几乎所有的蛋白，但由于无法彻底去除而不允许用在制药行业中；酸，如70%甲酸[9]，可以破坏
-8878.3. Takayama, H. et al. 2008. High-level expression, single-step immunoaffinity purification and characterization of human tetraspanin membrane protein CD81. PLoS ONE 3: e2314 (DOI: 10.1371/journal.pone.0002314).4. Zhong, M. and Molday, R.S. 2010
microarrays: performing multiplex immunoassays on a single chip. A n a l.C h em . 75, 4368-4372. 25. Kersten, B., Wanker, E. E., Hoheisel, J. D., and Angenendt, P. (2005) Multiplexing approaches in protein microarray technology. E x p e r t R ev . P r
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