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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20℃ to -80℃
- 保质期:
12个月
- 英文名:
Recombinant Human ABL1 / JTK7 / p150 Protein (GST Tag)
- 库存:
99
- 供应商:
北京义翘神州科技股份有限公司
- 规格:
1.00 mg/20.00 µg/50.00 µg/500.00 µg
| 规格: | 1.00 mg | 产品价格: | ¥27875.0 |
|---|---|---|---|
| 规格: | 20.00 µg | 产品价格: | ¥2570.0 |
| 规格: | 50.00 µg | 产品价格: | ¥3350.0 |
| 规格: | 500.00 µg | 产品价格: | ¥0.0 |
蛋白名称:c-Abl/ABL1蛋白, c-Abl/ABL1 protein
蛋白构建:A DNA sequence encoding the amino acid sequence (Pro 137-Ser 554) of human ABL1 isoform B (NP_009297.2) was fused with the GST tag at the N-terminus.
表达宿主:Baculovirus-Insect Cells
蛋白纯度:> 75 % as determined by SDS-PAGE
蛋白活性:The specific activity was determined to be 240 nmol/min/mg using synthetic Abl peptide (EAIYAAPFAKKK) as substrate.
蛋白内毒素:< 1.0 EU per μg of the protein as determined by the LAL method
预测N端:Met
蛋白分子量:The recombinant human ABL1/GST chimera consists of 645 amino acids and predicts a molecular mass of 74 kDa. It migrates as an approximately 65 kDa band in SDS-PAGE under reducing conditions.
蛋白NP号:NP_009297.2
蛋白氨基酸序列:Pro137-Ser554
蛋白标签:N-GST
蛋白保存条件:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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文献和实验GST融合蛋白的准备 Preparation of Glutathione-S-Transferase (GST) Fusion Proteins
overnight at 37°C with shaking. Although variations of GST fusion protein expression vectors are available, the most commonly used versions include the sequence encoding the GST moiety followed by a multiple cloning site; an IPTG-inducible
Strategies to Optimize Protein Expression in E. coli
Peng, L., Xu, Z.N., Fang, X.M., Wang, F., Yang, S., and Cen, P.L. 2004. Preferential codons enhancing the expression level of human beta‐defensin‐2 in recombinant Escherichia coli. Protein Pept. Lett. 11:339
Functional Assays on High‐Content Protein Microarrays
Figure 1. Anti‐GST image of over 9000 unique human proteins on a ProtoArray human protein microarray. One of the subarrays is enlarged to show the subarray layout. Multiple control proteins are shown. These control proteins help to orient the array
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