产品封面图

CAL 27 人舌鳞癌细胞

收藏
  • 询价
  • ATCC 传代
  • 2026年01月04日
    avatar
  • 企业认证

    点击 QQ 联系

    • 详细信息
    • 询价记录
    • 文献和实验
    • 技术资料
    • 细胞类型

      普通细胞株-科研实验

    • 物种来源

    • 运输方式

      常温或干冰

    • 生长状态

      良好

    • 供应商

      复祥生物

    • 库存

      大量

    CAL 27 人舌鳞癌细胞

    DMEM+10%胎牛血清。细胞货期8-10个工作日

    上海复祥生物提供 ATCC 细胞|细胞系|细胞株|肿瘤细胞|细胞|贴壁细胞|悬浮细胞|,细胞库管理规范,提供的细胞株背景清楚,提供参考文献和最优培养条件,网站上有细胞照片,欢迎各位老师来电咨询!联系电话400-821-8510 手机15000266580
    邮箱xiangfbio@163.com.
    详情请登陆http://www.xiangbio.com/
    ATCC® Number: CRL-2095™ Price:
    Designations: CAL 27
    Depositors: C Cardona
    Biosafety Level: 1
    Shipped: frozen
    Medium & Serum: See Propagation
    Growth Properties: adherent
    Organism: Homo sapiens (human)
    Morphology: epithelial
    Source: Organ: tongue
    Disease: squamous cell carcinoma
    Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
    Isolation: Isolation date: 1982
    Tumorigenic: Yes
    DNA Profile (STR): Amelogenin: X
    CSF1PO: 10,12
    D13S317: 10,11
    D16S539: 11,12
    D5S818: 11,12
    D7S820: 10
    THO1: 6,9.3
    TPOX: 8
    vWA: 14,17
    Cytogenetic Analysis: aneuploid; modal number = 43
    Age: 56 years
    Gender: male
    Ethnicity: Caucasian
    Comments: Cal 27 was established in 1982 by J. Gioanni (Centre Antoine Lacassagne, Nice Cedex, France) from tissue taken prior to treatment from a 56 year old Caucasian male with a lesion of the middle of the tongue.
    CAL 27 cells are epithelial, polygonal with a highly granular cytoplasm.
    Immunocytochemical studies show strong positive staining with anti keratin antibodies.
    The cells do not grow well in semi-solid medium.
    Marked inhibition of thymidine incorporation was observed in the presence of VP16 (etoposide), CCNU (1-[2-chloroethyl]-3-cyclohexyl-1-nitrosourea), VM26 (teniposide), ADM (adriamycin), CPA (cyclophosphamide), and MTX (methotrexate).
    CAL 27 cells were resistant to treatment with VDS (vindesine sulfate), CDP (cis-platinum) or ACTD (actinomycin D).
    A culture submitted to the ATCC in December 1993 was found to be contaminated with mycoplasma. Progeny were cured by a 21-day treatment with BM Cycline.
    Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
    Temperature: 37.0°C
    Subculturing: Protocol: Remove spent medium, add fresh 0.25% trypsin, 0.53 mM EDTA solution, rinse and remove trypsin. Add fresh trypsin and let the culture sit at room temperature (or at 37C) until the cells detach. Add fresh medium, aspirate and dispense into new flasks.
    Subcultivation Ratio: A subcultivation ratio of 1:6 is recommended
    Medium Renewal: Every 2 to 3 days
    Preservation: Freeze medium: Complete growth medium, 95%; DMSO, 5%
    Storage temperature: liquid nitrogen vapor temperature
    Doubling Time: 35 hrs
    Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
    recommended serum:ATCC 30-2020
    References: 23521: Gioanni J, et al. Two new human tumor cell lines derived from squamous cell carcinomas of the tongue: establishment, characterization and response to cytotoxic treatment. Eur. J. Cancer Clin. Oncol. 24: 1445-1455, 1988. PubMed: 3181269

    风险提示:丁香通仅作为第三方平台,为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全,合理判断,谨慎选购商品,商家和用户对交易行为负责。对于医疗器械类产品,请先查证核实企业经营资质和医疗器械产品注册证情况。

    • 作者
    • 内容
    • 询问日期
    图标文献和实验
    相关实验
    • 实验27 兔(或大白鼠)的循环系统

      锁骨下动脉。但不同个体大动脉弓的分支情况有所不同(图27-1)。   1.无名动脉:为1条短而粗的血管,具有两大分支,即右锁骨下动脉和右总颈动脉。   (1)右锁骨下动脉:到达腋部时可成为腋动脉,伸入上臂后形成右肱动脉。   (2)右总颈动脉:沿气管右侧前行至口角处,分为内颈动脉和外颈动脉。内颈动脉绕向外侧背方,但其主干进入脑颅,供应脑的血液;另有一小分支分布于颈部肌肉。外颈动脉的位置靠内侧,前行分成几个小支,供应头部颜面部和的血液(不须细找)。   2.左

    • 实验动物饲料、饮水要求量和排便排尿量

      ~53升  排便量克/天27.2~40.8公斤  排尿量毫升/天11.4~19.0升  发热量(cal)只/小时3120猪饮料要求量/克/只/天1.8~3.6公斤  饮水要求量毫升/只/天3.8~5.7升  排便量克/天2.7~3.2公斤  排尿量毫升/天1.9~3.8升  发热量(cal)只/小时山羊饮料要求量/克/只/天0.7~4.5公斤  饮水要求量毫升/只/天1~4升  排便量克/天1.4~2.7公斤  排尿量毫升/天 0.7~2.0  发热量(cal)只/小时1365~2145绵羊饮料

    • 中国科学院细胞库可供应细胞目录

      ]  TCHu125  人  甲状腺鳞癌细胞  1000元 SW620  TCHu101  人  结肠癌细胞  800元 T24  TCHu 55  人  膀胱移行细胞癌细胞  400元 T-47D  TCHu 87  人  乳腺管癌细胞  400元 Tca-8113  TCHu 77  人  舌鳞癌细胞  400元 TE-1  TCHu 89  人  食管癌细胞  400元 TE-10  TCHu 90  人  食管癌细胞  400元 TE-11  TCHu 91  人  食管

    图标技术资料

    暂无技术资料 索取技术资料

    同类产品报价

    产品名称
    产品价格
    公司名称
    报价日期
    ¥1680
    优利科(上海)生命科学有限公司
    2025年12月08日询价
    ¥2800
    上海彩佑实业有限公司
    2025年07月11日询价
    ¥900
    上海淳麦生物科技有限公司
    2025年07月12日询价
    ¥1800
    上海晶风生物科技有限公司
    2025年03月26日询价
    询价
    上海复祥生物科技有限公司
    2025年06月29日询价
    CAL 27 人舌鳞癌细胞
    询价