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CRL-2755 EMT6 大鼠乳腺癌细胞

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  • EMT6
  • 2025年12月16日
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    CRL-2755 EMT6 大鼠乳腺癌细胞,ATCC 细胞|细胞系|细胞株|肿瘤细胞|细胞|贴壁细胞|悬浮细胞;细胞库管理规范,提供的细胞株背景清楚,提供参考文献和培养条件!

    CRL-2755 EMT6 大鼠乳腺癌细胞 的详细介绍
    CRL-2755 EMT6 大鼠乳腺癌细胞
    ATCC® Number: CRL-2755™ Price: $355.00
    Designations: EMT6
    Depositors: S Rockwell
    Biosafety Level: 1
    Shipped: frozen
    Medium & Serum: See Propagation
    Growth Properties: adherent
    Organism: Mus musculus (mouse)
    Morphology: epithelial
    Source: Organ: breast
    Strain: BALB/cCrgl
    Disease: mammary carcinoma
    Cell Type: epithelial
    Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
    Isolation: Isolation date: 1971
    Tumorigenic: Yes
    Gender: female
    Comments: EMT6 was established from a transplantable murine mammary carcinoma that arose in a BALB/cCRGL mouse after implantation of a hyperplastic mammary alveolar nodule. The resulting tumor line (named KHJJ) was propagated in BALB/cKa mice and adapted to tissue culture after the 25th animal passage, and the cell line was named EMT. EMT6 is a clonal isolate of EMT isolated in 1971 at Stanford University. The EMT6 cell line can be grown either in animals as a tumor or in tissue culture. Cells derived from tumors have a reported in vitro plating efficiency of 30%. Cell grown in tissue culture reportedly have a plating efficiency of 70%.
    Propagation: ATCC complete growth medium: Waymouth's MB 752/1 Medium with 2mM L-glutamine, 85%; fetal bovine serum, 15%
    Atmosphere: air, 95%; carbon dioxide (CO2), 5%
    Temperature: 37.0°C
    Subculturing: Protocol:
    1. Remove and discard culture medium.
      • Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
        • Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
          Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
          • Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
            • Add appropriate aliquots of the cell suspension to new culture vessels.
              • Incubate cultures at 37°C.

    Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:10 is recommended
    Medium Renewal: Every 2 to 3 days

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