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CRL-7869 Hs 738.St/Int 人成纤维细胞

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  • ATCC
  • 美国
  • Hs 738.St/Int
  • 2025年11月24日
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      人成纤维细胞

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    • 器官来源

    • 运输方式

      常温

    • 年限

      2020

    • 生长状态

      正常

    • 库存

      大量

    • 是否是肿瘤细胞

    CRL-7869 Hs 738.St/Int 人成纤维细胞

    ATCC® Number: CRL-7869™ Price: $429.00
    Designations: Hs 738.St/Int
    Biosafety Level: 1
    Shipped: frozen
    Medium & Serum: See Propagation
    Growth Properties: adherent
    Organism: Homo sapiens (human)
    Morphology: fibroblast
    产品细节图片1
    Source: Organ: mixed; stomach; intestine
    Disease: normal
    Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
    DNA Profile (STR): Amelogenin: X,Y
    CSF1PO: 10,11
    D13S317: 8,11
    D16S539: 9,11
    D5S818: 11,13
    D7S820: 8,12
    THO1: 7,9.3
    TPOX: 8
    vWA: 16,18
    Cytogenetic Analysis: modal number = 46; range = 44 to 92
    Age: 18 weeks gestation fetus
    Gender: male
    Ethnicity: Caucasian
    Comments: Part of the NBL Cell Line Collection. This cell line is neither produced nor fully characterized by ATCC . We do not guarantee that it will maintain a specific morphology, purity, or any other property upon passage.
    Please see the NBL Repository description.
    Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
    Atmosphere: air, 95%; carbon dioxide (CO2), 5%
    Temperature: 37.0°C
    Subculturing: Protocol: Remove medium, and rinse with 0.25% trypsin, 0.53 mM EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.
    Subcultivation Ratio: A subcultivation ratio of 1:2 is recommended
    Medium Renewal: Every 2 to 3 days
    Preservation: Freeze medium: Complete growth medium 95%; DMSO, 5%
    Storage temperature: liquid nitrogen vapor termperature
    Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
    recommended serum:ATCC 30-2020
    ATCC® Number: CRL-7869™ Price: $429.00
    Designations: Hs 738.St/Int
    Biosafety Level: 1
    Shipped: frozen
    Medium & Serum: See Propagation
    Growth Properties: adherent
    Organism: Homo sapiens (human)
    Morphology: fibroblast


    Source: Organ: mixed; stomach; intestine
    Disease: normal
    Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

    DNA Profile (STR): Amelogenin: X,Y
    CSF1PO: 10,11
    D13S317: 8,11
    D16S539: 9,11
    D5S818: 11,13
    D7S820: 8,12
    THO1: 7,9.3
    TPOX: 8
    vWA: 16,18
    Cytogenetic Analysis: modal number = 46; range = 44 to 92
    Age: 18 weeks gestation fetus
    Gender: male
    Ethnicity: Caucasian
    Comments: Part of the NBL Cell Line Collection. This cell line is neither produced nor fully characterized by ATCC . We do not guarantee that it will maintain a specific morphology, purity, or any other property upon passage.
    Please see the NBL Repository description.
    Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
    Atmosphere: air, 95%; carbon dioxide (CO2), 5%
    Temperature: 37.0°C
    Subculturing: Protocol: Remove medium, and rinse with 0.25% trypsin, 0.53 mM EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.
    Subcultivation Ratio: A subcultivation ratio of 1:2 is recommended
    Medium Renewal: Every 2 to 3 days
    Preservation: Freeze medium: Complete growth medium 95%; DMSO, 5%
    Storage temperature: liquid nitrogen vapor termperature
    Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002

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