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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 供应商:
ATCC
- 品系:
查看相关资料
- 组织来源:
人成纤维细胞
- 相关疾病:
查看相关资料
- 物种来源:
人
- 免疫类型:
查看相关资料
- 器官来源:
人
- 运输方式:
常温
- 年限:
2020
- 生长状态:
正常
- 库存:
大量
- 是否是肿瘤细胞:
否
CRL-7869 Hs 738.St/Int 人成纤维细胞
| ATCC® Number: | CRL-7869™ | Price: | $429.00 | |||||||||||||||||||||||||||||||||||||||||
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Designations: Hs 738.St/Int
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Homo sapiens (human)
Morphology: fibroblast
Source: Organ: mixed; stomach; intestine
Disease: normal
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
DNA Profile (STR): Amelogenin: X,Y
CSF1PO: 10,11
D13S317: 8,11
D16S539: 9,11
D5S818: 11,13
D7S820: 8,12
THO1: 7,9.3
TPOX: 8
vWA: 16,18
Cytogenetic Analysis: modal number = 46; range = 44 to 92
Age: 18 weeks gestation fetus
Gender: male
Ethnicity: Caucasian
Comments: Part of the NBL Cell Line Collection. This cell line is neither produced nor fully characterized by ATCC . We do not guarantee that it will maintain a specific morphology, purity, or any other property upon passage.
Please see the NBL Repository description.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Subculturing: Protocol: Remove medium, and rinse with 0.25% trypsin, 0.53 mM EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation Ratio: A subcultivation ratio of 1:2 is recommended
Medium Renewal: Every 2 to 3 days
Preservation: Freeze medium: Complete growth medium 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor termperature
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
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细胞、贴壁 DMEM,10%FBSA431 CRL-1555 人 表皮细胞癌 上皮细胞、贴壁 DMEM,10%FBSA549 CCL-185 人 肺癌 贴壁、上皮样 F12,10%FBSA9 CRL-6319 小鼠 结缔组织 成纤维细胞、贴壁 DMEM,10% FBS+AR42J CRL-1492 大鼠 胰腺肿瘤 贴壁、上皮样 Ham'sF-12K, 20%FBSAtT-20 CCL-89 小鼠 垂体肿瘤 上皮细胞、贴壁 F-10, 15% HS和2.5% FBSB95-8 CRL-2311 绒猴 EB
一、细胞多能性胚胎干细胞产生于小鼠胚泡1.表达绿色荧光蛋白(EGFP)的B5-ES细胞。由DrNgy的实室制备。2.D3-ATCC;CRL-1934我们得到时大约传了17代。3.J1-由DrJenish的实室友情提供。我们得到时大约传了7-9代。4.J1rtTA-rtTA表达J1细胞,由DrJenish的实室友情提供。5.表达黄色荧光蛋白的YC5-ES细胞,由DrNgy的实室提供。二、一般培养--维持ES细胞处于未分化状态ES细胞培养用含有ESGRO(白血病抑制因子)的高糖培养基来阻止细胞









