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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Formaldehide treated whole cells of Legionella pneumophila strain Philadelphia 1. Immunized 7 times at two weeks intervals.
- 亚型:
IgG
- 形态:
Liquid
- 保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性:
Polyclonal
- 标记物:
Unconjugated
- 适应物种:
Legionella pneumophila
- 保质期:
12 months from the shipping date of the product.
- 目录编号:
GTX64135
- 级别:
Primary Antibodies
- 库存:
Available
- 供应商:
GeneTex
- 宿主:
Rabbit
- 应用范围:
ICC/IF, ELISA, Agglutination, IHC
- 浓度:
Batch dependent (Please refer to the vial label for the specific concentration.)
- 靶点:
Legionella pneumophila
- 抗体英文名:
Legionella pneumophila antibody
- 抗体名:
Legionella pneumophila 抗体
- 规格:
200 μl
Immunofluorescent staining of Legionella pneumophila in the infected HEK293 cells using anti-Legionella pneumophila antibody. HEK293 cells were infected with Legionella pneumophila strain Philadelphia1 fixed with 4% formaldehyde and reacted with the anti-Legionella pneumophila antibody at 1/10,000 dilution. As the second antibody, goat Rodamine Red X conjugated anti-rabbit IgG antibody was used at 1/10,000 dilution. DNA was stained with Hoechst 33342 (center) and the images were merged with that of differential interference contrast microscope (right).
Titration of antibody reactivity of anti-Legionella pneumophila antiserum by direct ELISA Plate was coated with 100 μl of 109 cells/ml per well and 100 μl of the antiserum at the indicated dilution was added to each well and incubated. After washing, goat anti-rabbit-IgG conjugated with HRP was added as 2nd antibody. Color was developed with TMB as substrate. "pre" is preimmune serum and "Immunized" is immunized serum
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文献和实验Natural Biofilm Formation with Legionella pneumophila
Biofilm formation could be studied in various conditions. Most of the studies with Legionella pneumophila used monospecies biofilm in culture media. In some cases, it is important to study bacteria in conditions more close to environmental
Constructing Unmarked Gene Deletions in Legionella pneumophila
. Here we describe a three-phase approach for generating unmarked deletions in Legionella pneumophila by constructing a mutant allele in E. coli using λ-Red recombination, so-called recombineering; transferring the allele onto the L. pneumophila chromosome by natural
Enrichment of Outer Membrane Vesicles Shed by Legionella pneumophila
The production of outer membrane vesicles (OMVs) is a widespread phenomenon employed by bacteria to secrete cell envelope components into the environment. A contribution of Legionella pneumophila OMVs to the pathogenesis of Legionnaires
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