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- 详细信息
- 技术资料
- 免疫原:
Formaldehide treated whole cells of Legionella pneumophila strain Philadelphia 1. Immunized 7 times at two weeks intervals.
- 亚型:
IgG
- 形态:
Liquid
- 保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性:
Polyclonal
- 标记物:
Unconjugated
- 适应物种:
Legionella pneumophila
- 保质期:
12 months from the shipping date of the product.
- 目录编号:
GTX64135
- 级别:
Primary Antibodies
- 库存:
Available
- 供应商:
GeneTex
- 宿主:
Rabbit
- 应用范围:
ICC/IF, ELISA, Agglutination, IHC
- 浓度:
Batch dependent (Please refer to the vial label for the specific concentration.)
- 靶点:
Legionella pneumophila
- 抗体英文名:
Legionella pneumophila antibody
- 抗体名:
Legionella pneumophila 抗体
- 规格:
200 μl
Immunofluorescent staining of Legionella pneumophila in the infected HEK293 cells using anti-Legionella pneumophila antibody. HEK293 cells were infected with Legionella pneumophila strain Philadelphia1 fixed with 4% formaldehyde and reacted with the anti-Legionella pneumophila antibody at 1/10,000 dilution. As the second antibody, goat Rodamine Red X conjugated anti-rabbit IgG antibody was used at 1/10,000 dilution. DNA was stained with Hoechst 33342 (center) and the images were merged with that of differential interference contrast microscope (right).
Titration of antibody reactivity of anti-Legionella pneumophila antiserum by direct ELISA Plate was coated with 100 μl of 109 cells/ml per well and 100 μl of the antiserum at the indicated dilution was added to each well and incubated. After washing, goat anti-rabbit-IgG conjugated with HRP was added as 2nd antibody. Color was developed with TMB as substrate. "pre" is preimmune serum and "Immunized" is immunized serum
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