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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Recombinant protein encompassing a sequence within the center region of human Transgelin. The exact sequence is proprietary.
- 亚型:
IgG
- 形态:
Liquid
- 保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性:
Polyclonal
- 标记物:
Unconjugated
- 适应物种:
Human, Mouse, Rat
- 保质期:
12 months from the shipping date of the product.
- 抗原来源:
Human
- 目录编号:
GTX101608
- 级别:
Primary Antibodies
- 库存:
Available
- 供应商:
GeneTex
- 宿主:
Rabbit
- 应用范围:
WB, ICC/IF, IHC-P, IP
- 浓度:
1.22 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点:
Transgelin
- 抗体英文名:
Transgelin antibody
- 抗体名:
Transgelin 抗体
- 规格:
100 μl/25 μl
| 规格: | 100 μl | 产品价格: | ¥4000.0 |
|---|---|---|---|
| 规格: | 25 μl | 产品价格: | ¥1700.0 |
Non-transfected (–) and transfected (+) HeLa whole cell extracts (15 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Transgelin antibody (GTX101608) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Transgelin antibody detects Transgelin protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse smooth muscle.
Transgelin stained by Transgelin antibody (GTX101608) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Transgelin antibody detects TAGLN protein by western blot analysis.
A. 30 μg Rat-2 whole cell lysate/extract
12% SDS-PAGE
Transgelin antibody (GTX101608) dilution: 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Transgelin antibody (GTX101608) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Transgelin antibody detects Transgelin protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% PFA at RT for 15 min.
Green: Transgelin protein stained by Transgelin antibody (GTX101608) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Hoechst 33342 staining.
Transgelin antibody detects Transgelin protein at cytoplasm in rat stomach by immunohistochemical analysis.
Sample: Paraffin-embedded rat stomach.
Transgelin antibody (GTX101608) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Immunoprecipitation of Transgelin protein from HeLa whole cell extracts using 5 μg of Transgelin antibody (GTX101608).
Western blot analysis was performed using Transgelin antibody (GTX101608).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
Transgelin antibody detects TAGLN protein by western blot analysis.
A. 30 μg C8D30 whole cell lysate/extract
12% SDS-PAGE
Transgelin antibody (GTX101608) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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文献和实验Stratman AN et al., Commun Biol 2020 (PMID:33277595)
Hao W et al., Life Sci 2017 (PMID:29208463)
Hampl V et al., EMBO Mol Med 2013 (PMID:23853104)
Morita T et al., J Vet Med Sci 2014 (PMID:24694942)
Gautam J et al., Sci Rep 2016 (PMID:27808256)
Lin LH et al., Electrophoresis 2014 (PMID:24723343)
Andrew H Smith et al., JVS Vasc Sci 2020 (PMID:33554153)
Generation of Antibody Molecules Through Antibody Engineering
been overcome to a large extent using genetic-engineering techniques to produce chimeric mouse/human and completely human antibodies. Such an approach is particularly suitable because of the domain structure of the antibody molecule ( 2 ), where functional
The importance of antibody molecules was first recognized in the 1890s, when it was shown that immunity to tetanus and diphtheria was caused by antibodies against the bacterial exotoxins (1 ). Around the same time, it was shown that antisera
General comments: Antibodies, like most proteins, do not like to be freeze-thawed. Avoid repetitive freezing of your solution. The best way to store your antibody is to keep a high protein concentration (>1 mg/ml), add some protease
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