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- 2025年10月16日
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| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-11208R |
| 英文名称 | SOX4 Rabbit pAb |
| 中文名称 | 核转录因子SOX4抗体 |
| 英文别名 | SOX4; SOX 4; SOX-4; Ecotropic viral integration site 16; EVI 16; EVI16; SRY(sex determining region Y) box 4; SRY box containing gene 4; SRY related HMG box gene 4; SOX4_HUMAN. |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=2ug/Test Not yet tested in other applications. |
| 交叉反应 | Human, Mouse, Rat (Chicken) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human SOX4 |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 52 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Epigenetics and Nuclear Signaling > Transcription > Domain Families > Developmental Families Epigenetics and Nuclear Signaling > Transcription > Domain Families > HMG Box Neuroscience > Cell Type Marker > Neural Stem Cell marker Neuroscience > Neurology process > Neural Signal Transduction Stem Cells > Neural Stem Cells > Intracellular |
| 亚基 | Interacts with UBE2I. |
| 亚细胞定位 | Nuclear |
| 组织特异性 | Testis, brain, and heart. |
| 相似性 | Contains 1 HMG box DNA-binding domain. |
| 功能 | The SOX (SRY-box containing gene) gene family encodes a group of transcription factors defined by the conserved high motility group (HMG) DNA-binding domain. They are involved in the regulation of embryonic development and in the determination of cell fate. SOX4 shows a high degree of sequence homology with SOX11. Both are expressed in the CNS. SOX4 and SOX11 expression increase together in the developing CNS (with some exceptions: external granule layer of the cerebellum). SOX4 knock-out mice have been shown to die of a heart defect half way through gestation with no observable CNS phenotype, although it has been proposed that SOX11 compensates for the absence of SOX4. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | Sox genes comprise a family of genes that are related to the mammalian sex determining gene SRY. These genes similarly contain sequences that encode for the HMG-box domain, which is responsible for the sequence-specific DNA-binding activity. Sox genes encode putative transcriptional regulators implicated in the decision of cell fates during development and the control of diverse developmental processes. The highly complex group of Sox genes cluster at least 40 different loci that rapidly diverged in various animal lineages. At present, 30 Sox genes have been identified. Members of this family have been shown to be conserved during evolution and to play key roles during animal development. Some are involved in human diseases, including sex reversal. |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
| {IF} | {1:100-500} |
| {Flow-Cyt} | {2ug/Test} |
Testis (Mouse) Lysate at 40 ug
Cerebrum (Mouse) Lysate at 40 ug
Primary: Anti-SOX4 (bs-11208R) at 1/500 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 52 kD
Observed band size: 52 kD
Primary Antibody (green line): Rabbit Anti-SOX4 antibody (bs-11208R)
Dilution: 2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-APC
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
Lane 1: Mouse Heart tissue lysates
Lane 2: Human 293T cell lysates
Primary: Anti-SOX4 (bs-11208R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 52 kDa
Observed band size: 46 kDa
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文献和实验[IF={{ 5.201 }}] {Jingxuan Li. et al. Long Non-coding RNA H19 Regulates Porcine Satellite Cell Differentiation Through miR-140-5p/SOX4 and DBN1. Front Cell Dev Biol. 2020; 8: 518724} {WB} {Pig}
[IF={{ 4.8 }}] {Yanmiao Ma. et al.Cornua cervi degelatinatum inhibits breast cancer stem-like cell properties and metastasis via miR-148a-3p-mediated TGF-β/Smad2 pathway..JOURNAL OF ETHNOPHARMACOLOGY.2025 Mar 27:346:119709.} {Western blot,IF} {Human}
[IF={{ 4.8 }}] {Yanmiao Ma. et al.Cornua cervi degelatinatum inhibits breast cancer stem-like cell properties and metastasis via miR-148a-3p-mediated TGF-β/Smad2 pathway..JOURNAL OF ETHNOPHARMACOLOGY.2025 Mar 27:346:119709.} {Western blot,IHC} {Human}
[IF={{ 4.599 }}] {Juan Tan. et al. Circ_0124644 Serves as a ceRNA for miR-590-3p to Promote Hypoxia-Induced Cardiomyocytes Injury via Regulating SOX4. Front Genet. 2021; 12: 667724} {WB} {Human}
[IF={{ 2.8 }}] {Mengxin Hu. et al. Establishment and characterization of cisplatin-resistant cell lines from canine mammary gland tumors. THERIOGENOLOGY. 2024 Mar;217:103} {WB} {Dog}
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