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- bs-11208R
- 2026年10月13日
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| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-11208R |
| 英文名称 | SOX4 Rabbit pAb |
| 中文名称 | 核转录因子SOX4抗体 |
| 英文别名 | SOX4; SOX 4; SOX-4; Ecotropic viral integration site 16; EVI 16; EVI16; SRY(sex determining region Y) box 4; SRY box containing gene 4; SRY related HMG box gene 4; SOX4_HUMAN. |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=2ug/Test Not yet tested in other applications. |
| 交叉反应 | Human, Mouse, Rat (Chicken) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human SOX4 |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 52 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Epigenetics and Nuclear Signaling > Transcription > Domain Families > Developmental Families Epigenetics and Nuclear Signaling > Transcription > Domain Families > HMG Box Neuroscience > Cell Type Marker > Neural Stem Cell marker Neuroscience > Neurology process > Neural Signal Transduction Stem Cells > Neural Stem Cells > Intracellular |
| 亚基 | Interacts with UBE2I. |
| 亚细胞定位 | Nuclear |
| 组织特异性 | Testis, brain, and heart. |
| 相似性 | Contains 1 HMG box DNA-binding domain. |
| 功能 | The SOX (SRY-box containing gene) gene family encodes a group of transcription factors defined by the conserved high motility group (HMG) DNA-binding domain. They are involved in the regulation of embryonic development and in the determination of cell fate. SOX4 shows a high degree of sequence homology with SOX11. Both are expressed in the CNS. SOX4 and SOX11 expression increase together in the developing CNS (with some exceptions: external granule layer of the cerebellum). SOX4 knock-out mice have been shown to die of a heart defect half way through gestation with no observable CNS phenotype, although it has been proposed that SOX11 compensates for the absence of SOX4. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | Sox genes comprise a family of genes that are related to the mammalian sex determining gene SRY. These genes similarly contain sequences that encode for the HMG-box domain, which is responsible for the sequence-specific DNA-binding activity. Sox genes encode putative transcriptional regulators implicated in the decision of cell fates during development and the control of diverse developmental processes. The highly complex group of Sox genes cluster at least 40 different loci that rapidly diverged in various animal lineages. At present, 30 Sox genes have been identified. Members of this family have been shown to be conserved during evolution and to play key roles during animal development. Some are involved in human diseases, including sex reversal. |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
| {IF} | {1:100-500} |
| {Flow-Cyt} | {2ug/Test} |
Paraformaldehyde-fixed, paraffin embedded (human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SOX4) Polyclonal Antibody, Unconjugated (bs-11208R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SOX4) Polyclonal Antibody, Unconjugated (bs-11208R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human breast carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SOX4) Polyclonal Antibody, Unconjugated (bs-11208R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SOX4) Polyclonal Antibody, Unconjugated (bs-11208R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SOX4) Polyclonal Antibody, Unconjugated (bs-11208R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Sample:
_x000D_ Testis (Mouse) Lysate at 40 ug
_x000D_ Cerebrum (Mouse) Lysate at 40 ug
_x000D_ Primary: Anti-SOX4 (bs-11208R) at 1/500 dilution
_x000D_ Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
_x000D_ Predicted band size: 52 kD
_x000D_ Observed band size: 52 kD
_x000D_ Testis (Mouse) Lysate at 40 ug
_x000D_ Cerebrum (Mouse) Lysate at 40 ug
_x000D_ Primary: Anti-SOX4 (bs-11208R) at 1/500 dilution
_x000D_ Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
_x000D_ Predicted band size: 52 kD
_x000D_ Observed band size: 52 kD
Blank control:Molt4.
_x000D_ Primary Antibody (green line): Rabbit Anti-SOX4 antibody (bs-11208R)
_x000D_ Dilution: 2μg /10^6 cells;
_x000D_ Isotype Control Antibody (orange line): Rabbit IgG .
_x000D_ Secondary Antibody : Goat anti-rabbit IgG-APC
_x000D_ Dilution: 1μg /test.
_x000D_ Protocol
_x000D_ The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed._x000D_
_x000D_ Primary Antibody (green line): Rabbit Anti-SOX4 antibody (bs-11208R)
_x000D_ Dilution: 2μg /10^6 cells;
_x000D_ Isotype Control Antibody (orange line): Rabbit IgG .
_x000D_ Secondary Antibody : Goat anti-rabbit IgG-APC
_x000D_ Dilution: 1μg /test.
_x000D_ Protocol
_x000D_ The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed._x000D_
Sample:
_x000D_ Lane 1: Mouse Heart tissue lysates
_x000D_ Lane 2: Human 293T cell lysates
_x000D_ Primary: Anti-SOX4 (bs-11208R) at 1/1000 dilution
_x000D_ Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
_x000D_ Predicted band size: 52 kDa
_x000D_ Observed band size: 46 kDa
_x000D_ _x000D_
_x000D_ Lane 1: Mouse Heart tissue lysates
_x000D_ Lane 2: Human 293T cell lysates
_x000D_ Primary: Anti-SOX4 (bs-11208R) at 1/1000 dilution
_x000D_ Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
_x000D_ Predicted band size: 52 kDa
_x000D_ Observed band size: 46 kDa
_x000D_ _x000D_
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SOX4 Rabbit pAb(bs-11208R)-50ul/100ul/200ul
￥1180 - 2800
