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Recombinant human HIST3H3 Protein
IgG
Liquid
Store at -20 °C for one year.
单克隆
无
Human,Mouse,Rat,Bee,
1年
bsm-33042M
999
北京博奥森生物技术有限公司
Mouse
WB=1:500-5000,IHC-P=1:400-800,IHC-F=1:400-800,IF=1:100-500,
1mg/ml
Histone H3/HIST3H3
组蛋白H3单克隆抗体
 Sample: K562 Cell (Human) Lysate at 40 ug Primary: Anti-Histone H3/HIST3H3(bsm-33042M)at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 15kD Observed band size: 15kD  Sample:Hela Cell (Human) Lysate at 40 ug Primary: Anti-Histone H3/HIST3H3(bsm-33042M)at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 15kD Observed band size: 15kD  Sample: NIH/3T3 Cell(Mouse) Lysate at 40 ug Primary: Anti-Histone H3/HIST3H3(bsm-33042M)at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 15kD Observed band size: 15kD  Sample: Large intestine(Rat) Lysate at 40 ug MCF-7(Human) Cell Lysate at 30 ug U2os(Human) Cell Lysate at 30 ug Primary: Anti-Histone H3/HIST3H3 (bsm-33042M) at 1/1000 dilution Secondary: IRDye800CW Goat Anti- Mouse IgG at 1/20000 dilution Predicted band size: 15 kD Observed band size: 15 kD  Sample: Small intestine (Mouse) Lysate at 40 ug Primary: Anti- Histone H3/HIST3H3 (bsm-33042M) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 15 kD Observed band size: 15 kD  Sample: Large intestine (Mouse) Lysate at 40 ug Primary: Anti- Histone H3/HIST3H3 (bsm-33042M) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 15 kD Observed band size: 15 kD  Sample: NIH/3T3(Mouse) Cell Lysate at 30 ug Primary: Anti- Histone H3/HIST3H3 (bsm-33042M) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution Predicted band size: 15 kD Observed band size: 15 kD  Paraformaldehyde-fixed, paraffin embedded (Rat liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (HIST3H3) Monoclonal Antibody, Unconjugated (bsm-33042M) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.  Paraformaldehyde-fixed, paraffin embedded (rat kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Histone H3 HIST3H3 (Nuclear Loading Control)) Monoclonal Antibody, Unconjugated (bsm-33042M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.  Paraformaldehyde-fixed, paraffin embedded (rat liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Histone H3 HIST3H3 (Nuclear Loading Control)) Monoclonal Antibody, Unconjugated (bsm-33042M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.  Paraformaldehyde-fixed, paraffin embedded (Rat colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (HIST3H3) Monoclonal Antibody, Unconjugated (bsm-33042M) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.  Paraformaldehyde-fixed, paraffin embedded (human gastric carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Histone H3 HIST3H3 (Nuclear Loading Control)) Monoclonal Antibody, Unconjugated (bsm-33042M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.  Paraformaldehyde-fixed, paraffin embedded (rat pancreas); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Histone H3 HIST3H3 (Nuclear Loading Control)) Monoclonal Antibody, Unconjugated (bsm-33042M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.  Paraformaldehyde-fixed, paraffin embedded (rat colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Histone H3 HIST3H3 (Nuclear Loading Control)) Monoclonal Antibody, Unconjugated (bsm-33042M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.  Paraformaldehyde-fixed, paraffin embedded (mouse pancreas); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Histone H3 HIST3H3 (Nuclear Loading Control)) Monoclonal Antibody, Unconjugated (bsm-33042M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.  Paraformaldehyde-fixed, paraffin embedded (mouse kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Histone H3 HIST3H3 (Nuclear Loading Control)) Monoclonal Antibody, Unconjugated (bsm-33042M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.  Paraformaldehyde-fixed, paraffin embedded (Rat colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Histone H3/HIST3H3) Monoclonal Antibody, Unconjugated (bsm-33042M) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody (bs-0296G-cy3) for 90 minutes, and DAPI for nuclei staining. 已发表文献 [IF=4.868]Zheng GH et al. Purple Sweet Potato Color Attenuates Kidney Damage by Blocking VEGFR2/ROS/NLRP3 Signaling in High-Fat Diet-Treated Mice. Oxid Med Cell Longev. 2019 Jan 22;2019:5189819. WB, Mouse [IF=2.35]Tian, Yue, et al. "Minocycline attenuates sevoflurane-induced cell injury via activation of Nrf2." International Journal of Molecular Medicine.WB, Human [IF=1.26]Zhao et al. Unfractionated heparin protects the protein C system against lipopolysaccharide-induced damagein vivoandin vitro. (2017) Exp.Ther.Med. 14:5515-5522WB, Human [IF=1.2]Zhao, Dongmei, et al. "Unfractionated heparin protects the protein C system against lipopolysaccharide‑induced damage in vivo and in vitro." Experimental and Therapeutic Medicine.WB, Human |
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