- ￥1180 - 2800
- Bioss
- bs-3428R
- 2025年10月16日
- 产品信息以Bioss网站为准
企业认证
相关产品推荐更多 >
Cytokeratin 19 Rabbit pAb, Cy3 conjugated(bs-1028R-Cy3)-100ul
￥2980
Anti-human SCFR / c-Kit / CD117 (LOP628 Biosimilar)(BIO0111SM)-1mg/5mg/20mg
￥3200
PLVAP Rabbit pAb(bs-12737R)-50ul/100ul/200ul
￥1180Histone H4 (Acetyl K16) Rabbit pAb, RBITC conjugated(bs-4707R-RBITC)-100ul
￥2980Synaptotagmin XII Rabbit pAb, BF350 conjugated(bs-11398R-BF350)-100ul
￥2980
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 应用范围：
产品信息以Bioss网站为准
- 规格：
50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-3428R |
| 英文名称 | phospho-Stat2 (Tyr690) Rabbit pAb |
| 中文名称 | 磷酸化信号转导和转录激活因子2抗体 |
| 英文别名 | Stat2(phospho-Tyr690); Stat2(phospho Y690); Stat2(phospho Tyr690); p-Stat2(Tyr690); signal transducers and activators of transduction2; Homo sapiens interferon alpha induced transcriptional activator; interferon alpha induced transcriptional activator; ISGF3; P113; signal transducer and activator of transcription 2 113kD; STAT113; STAT2_HUMAN; p113. |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 Not yet tested in other applications. |
| 交叉反应 | Mouse (Human, Rat, Dog, Horse) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated Synthesised phosphopeptide derived from human Stat2 around the phosphorylation site of Tyr690 |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 94 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Epigenetics and Nuclear Signaling > Nuclear Signaling Pathways > STATs Epigenetics and Nuclear Signaling > Transcription > Other factors Microbiology > Organism > Virus > RNA Virus > ssRNA positive strand virus > SARS Coronavirus Signal Transduction > Signaling Pathway > Nuclear Signaling > STATs |
| 亚基 | Interacts with ISGF3G/IRF-9 in the cytoplasm. Heterodimer with STAT1 upon IFN-alpha/beta induced phosphorylation. Interacts with CRSP2 and CRSP6. Interacts with Simian virus 5 protein V and Rabies virus phosphoprotein (By similarity). Can form a homodimer upon IFN-alpha induced phosphorylation. Interacts with IFNAR1; the interaction requires the phosphorylation of IFNAR1 at 'Tyr-466'. Interacts with IFNAR2. Interacts with dengue virus NS5; this interaction inhibits the phosphorylation of STAT2, and, when all viral proteins are present (polyprotein), targets STAT2 for degradation. Interacts with human cytomegalovirus/HHV-5 protein UL123; this interaction promotes viral growth. |
| 亚细胞定位 | Cytoplasm. Nucleus. Note=Translocated into the nucleus upon activation by IFN-alpha/beta. |
| 翻译后修饰 | Tyrosine phosphorylated in response to IFN-alpha. |
| 相似性 | Belongs to the transcription factor STAT family.
Contains 1 SH2 domain. |
| 功能 | Signal transducer and activator of transcription that mediates signaling by type I IFNs (IFN-alpha and IFN-beta). Following type I IFN binding to cell surface receptors, Jak kinases (TYK2 and JAK1) are activated, leading to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize, associate with ISGF3G/IRF-9 to form a complex termed ISGF3 transcription factor, that enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of interferon stimulated genes, which drive the cell in an antiviral state. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | The protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. In response to interferon (IFN), this protein forms a complex with STAT1 and IFN regulatory factor family protein p48 (ISGF3G), in which this protein acts as a transactivator, but lacks the ability to bind DNA directly. Transcription adaptor P300/CBP (EP300/CREBBP) has been shown to interact specifically with this protein, which is thought to be involved in the process of blocking IFN-alpha response by adenovirus. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2010]. |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
| {IF} | {1:100-500} |
Primary: Anti-Phospho-Stat2 (Tyr690)(bs-3428R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 94kD
Observed band size: 105kD
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验[IF={{ 8.4 }}] {Han Ying-Hao. et al. Peroxiredoxin II regulates exosome secretion from dermal mesenchymal stem cells through the ISGylation signaling pathway. CELL COMMUN SIGNAL. 2023 Dec;21(1):1-15} {WB} {Mouse}
[IF={{ 7.9 }}] {Yuzhuo Fan. et al. Immunoregulatory cryogel-based contact lenses for bacterial keratitis prevention and treatment. Cell Reports Physical Science. 2024 Sep;5:} {IHC} {Mouse}
[IF={{ 6.448 }}] {Collotta D et al. Baricitinib counteracts metaflammation, thus protecting against diet-induced metabolic abnormalities in mice. Mol Metab .2020 Sep;39:101009.} {WB} {Mouse}
[IF={{ 6.304 }}] {Chen Y et al. Dendritic cells-derived interferon-λ1 ameliorated inflammatory bone destruction through inhibiting osteoclastogenesis. Cell Death Dis. 2020 Jun 2;11(6):414.} {WB} {Mouse}
[IF={{ 6.291 }}] {Changjiang Liu. et al. Cypermethrin triggers YY1-mediated testosterone biosynthesis suppression. Ecotox Environ Safe. 2021 Dec;225:112792} {WB} {Rat}
。所以，我们建议使用微波炉或高压锅加热煮沸以达到最佳抗原修复效果。抗体：Phospho–Stat3 (Tyr705)(D3A7) XP. Rabbit mAb #9145样本：石蜡包埋人类肺部肿瘤样本煮沸设备：浴锅 (左)，微波炉 (中)，高压锅 (右)免疫染色—封闭在 IHC–P 中，我们建议在室温下在含有 Tween20 的 TBST 缓冲液和 5% 正常山羊血清 (NGS) 中封闭样品，时长 1 小时，以防止非特异性背景染色。在进行 IHC–F 时，在含有 0.3% Triton. X–100 的 1X
used in accordance withmanufacturer recommendationsSignalStain?DAB Substrate Kit #8059:IHC analysis of paraffin-embedded human breastcarcinoma using Phospho-Stat3 (Tyr705) (D3A7) XP? Rabbit mAb #9145. Chromogenicdetection was performed using #8059 (left
v468. chapter 4 WNT 细胞通路 检测细胞GSK3活性与表达水平
. Immunoblot using antibodies to phospho-Tyr279/216 can assess alterations in activity independent ofthe N-terminal phosphorylation, although phosphorylation atthis site is most likely to be constitutive (14) . 3. Immunoblot using antibodies to known
技术资料暂无技术资料 索取技术资料
