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- 技术资料
- 规格:
50ul/100ul/200ul
| 规格: | 50ul | 产品价格: | ¥1180.0 |
|---|---|---|---|
| 规格: | 100ul | 产品价格: | ¥1980.0 |
| 规格: | 200ul | 产品价格: | ¥2800.0 |
| 产品编号 | bs-3275R |
| 英文名称 | phospho-MEK6 (Ser207) Rabbit pAb |
| 中文名称 | 磷酸化丝裂原活化蛋白激酶MKK6抗体 |
| 英文别名 | MEK6(phospho S207); p-MEK6(phospho S207); MAP kinase kinase 6; MAPK/ERK kinase 6; MAPKK6; MEK6; Mitogen Activated Protein Kinase Kinase 6; MKK 6; MKK6; PRKMK6; SAPKK3; Dual specificity mitogen activated protein kinase kinase 6; MKK 6; Dual specificity mitogen activated protein kinase kinase 6; EC 2.7.12.2; MP2K6_HUMAN. |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 Not yet tested in other applications. |
| 交叉反应 | Mouse, Rat (Human) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthesised phosphopeptide derived from human MEK6 around the phosphorylation site of Ser207 |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 37 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Cancer > Signal transduction > Protein phosphorylation > Serine/threonine kinases > MAPK pathway Signal Transduction > Protein Phosphorylation > Ser / Thr Kinases > MAPK Pathway |
| 亚基 | Interacts with Yersinia yopJ._x000D_ |
| 亚细胞定位 | |
| 组织特异性 | Isoform 2 is only expressed in skeletal muscle. Isoform 1 is expressed in skeletal muscle, heart, and in lesser extent in liver or pancreas._x000D_ |
| 翻译后修饰 | Weakly autophosphorylated. Phosphorylated by TAOK2. Acetylation of Ser-207 and Thr-211 by Yersinia yopJ prevents phosphorylation and activation, thus blocking the MAPK signaling pathway._x000D_ |
| 相似性 | Belongs to the enoyl-CoA hydratase/isomerase family._x000D_ |
| 功能 | Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in MAP kinase p38 exclusively. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | Mitogen activated protein kinase kinase 6 (MEK6 or MKK6) belongs to the serine/threonine protein kinase family and the MAPK kinase subfamily (MAP2K, MKK or MEKs). MEK6, closely related to MEK3, catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in MAP kinase p38, thus activating it, in response to inflammatory cytokines and environmental stress.As an essential component of p38 MAP kinase mediated signal transduction pathway, this protein is involved in many cellular processes such as stress induced cell cycle arrest, transcription activation and apoptosis. |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
| {IF} | {1:100-500} |

Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Phospho-MEK6 (Ser207)) Polyclonal Antibody, Unconjugated (bs-3275R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Sample:
_x000D_ Muscle (Mouse) Lysate at 40 ug
_x000D_ Muscle (Rat) Lysate at 40 ug
_x000D_ Primary: Anti- Phospho-MEK6 (Ser207) (bs-3275R) at 1/1000 dilution
_x000D_ Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
_x000D_ Predicted band size: 37 kD
_x000D_ Observed band size: 37 kD
_x000D_
_x000D_ Muscle (Mouse) Lysate at 40 ug
_x000D_ Muscle (Rat) Lysate at 40 ug
_x000D_ Primary: Anti- Phospho-MEK6 (Ser207) (bs-3275R) at 1/1000 dilution
_x000D_ Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
_x000D_ Predicted band size: 37 kD
_x000D_ Observed band size: 37 kD
_x000D_

Sample:_x000D_
Lane 1: Mouse Large intestine tissue lysates_x000D_
Lane 2: Mouse Kidney tissue lysates_x000D_
Lane 3: Mouse Heart tissue lysates_x000D_
Primary: Anti-Phospho-MEK6 (Ser207) (bs-3275R) at 1/1000 dilution_x000D_
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution_x000D_
Predicted band size: 37 kDa_x000D_
Observed band size: 37 kDa
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MAP2K6 | MEK6 (p-S207) Rabbit pAb(bs-3275R)-50ul/100ul/200ul
¥1180 - 2800






