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Calreticulin Rabbit pAb(bs-591

3R)-50ul/100ul/200ul
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  • ￥1180 - 2800
  • Bioss已认证
  • bs-5913R
  • 2025年10月24日
  • 产品信息以Bioss网站为准
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北京博奥森生物技术有限公司
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      50ul/100ul/200ul

    规格：50ul产品价格：￥1180.0
    规格：100ul产品价格：￥1980.0
    规格：200ul产品价格：￥2800.0
    产品编号bs-5913R
    英文名称Calreticulin Rabbit pAb
    中文名称钙网蛋白抗体
    英文别名Autoantigen RO; CALR; CALR protein; CALR_HUMAN; cC1qR; CRP55; CRTC; Endoplasmic reticulum resident protein 60; ERp60; grp60; HACBP; RO; Sicca syndrome antigen A; SSA.
    产品应用WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, ELISA=1:5000-10000

    Not yet tested in other applications.
    Optimal working dilutions must be determined by the end user.

    交叉反应Human
    抗体来源Rabbit
    免疫原KLH conjugated synthetic peptide derived from human Calreticulin
    亚型IgG
    性状Liquid
    纯化方法affinity purified by Protein A
    克隆类型Polyclonal
    理论分子量44 kDa
    浓度1mg/ml
    储存液0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
    研究领域

    Tags & Cell Markers > Subcellular Markers > Organelles > ER

    亚基Monomer. Component of an EIF2 complex at least composed of CELF1/CUGBP1, CALR, CALR3, EIF2S1, EIF2S2, HSP90B1 and HSPA5. Interacts with PDIA3/ERp57 (By similarity). Interacts with NR3C1 and TRIM21. Interacts with GABARAP.
    亚细胞定位Endoplasmic reticulum lumen. Cytoplasm, cytosol. Secreted, extracellular space, extracellular matrix. Cell surface.
    相似性Belongs to the calreticulin family.
    保存条件Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
    注意事项This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
    背景资料Calreticulin is a highly conserved chaperone protein which resides primarily in the endoplasmic reticulum, and is involved in a variety of cellular processes, among them, cell adhesion. Additionally, it functions in protein folding quality control and calcium homeostasis. Calreticulin is also found in the nucleus, suggesting that it may have a role in transcription regulation. Systemic lupus erythematosus is associated with increased autoantibody titers against calreticulin. Recurrent mutations in calreticulin have been linked to various neoplasms, including the myeloproliferative type.[provided by RefSeq, May 2020]

     

    应用推荐稀释比例
    {WB}{1:500-2000}
    {IHC-P}{1:100-500}
    {IHC-F}{1:100-500}
    {IF}{1:100-500}
    {ELISA}{1:5000-10000}

     

    产品细节图片1
    Sample:
    Lane 1: Human Jurkat cell lysates
    Lane 2: Human HepG2 cell lysates
    Lane 3: Human MCF-7 cell lysates
    Primary: Anti-Calreticulin (bs-5913R) at 1/1000 dilution
    Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
    Predicted band size: 44 kDa
    Observed band size: 52 kDa
    产品细节图片2
    P‌‌araformaldehyde-fixed, paraffin embedded Human Lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Calreticulin Polyclonal Antibody, Unconjugated (bs-5913R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
    产品细节图片3
    P‌‌araformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Calreticulin Polyclonal Antibody, Unconjugated (bs-5913R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
    产品细节图片4
    P‌‌araformaldehyde-fixed, paraffin embedded Human Colon; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Calreticulin Polyclonal Antibody, Unconjugated (bs-5913R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
    产品细节图片5
    P‌‌araformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Calreticulin Polyclonal Antibody, Unconjugated (bs-5913R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.
    产品细节图片6
    P‌‌araformaldehyde-fixed, paraffin embedded Human Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Calreticulin Polyclonal Antibody, Unconjugated (bs-5913R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

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    图标文献和实验
    该产品被引用文献

    [IF={{ 9.7 }}] {Ziyang Zhu. et al. Aggregation-Induced Emission Photosensitizer/Bacteria Biohybrids Enhance Cerenkov Radiation-Induced Photodynamic Therapy by Activating Anti-Tumor Immunity for Synergistic Tumor Treatment. ACTA BIOMATER. 2023 Jun;:} {IHC,IF,ICC} {Mouse}

    [IF={{ 9.5 }}] {Ren-Jiang Kong. et al. Self-Delivery Photodynamic Re-educator Enhanced Tumor Treatment by Inducing Immunogenic Cell Death and Improving Immunosuppressive Microenvironments. ACS APPL MATER INTER. 2023;15(51):59165–59174} {IF} {Mouse}

    [IF={{ 9.4 }}] {Hongxin Liu. et al. Tumor associated antigens combined with carbon dots for inducing durable antitumor immunity. J COLLOID INTERF SCI. 2024 Nov;673:594} {IF} {Mouse}

    [IF={{ 9.4 }}] {Keze Hong. et al. A nanodrug provokes antitumor immune responses via synchronous multicellular regulation for enhanced cancer immunotherapy. J COLLOID INTERF SCI. 2024 Sep;:} {IF,FC} {Human,Mouse}

    [IF={{ 9.4 }}] {Yuqing Pan. et al. A tumor-targeting porphyrin-micelle with enhanced STING agonist delivery and synergistic photo-/immuno- therapy for cancer treatment. ACTA BIOMATER. 2024 Dec;:} {IF} {Mouse}

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    文献支持
    Calreticulin Rabbit pAb(bs-5913R)-50ul/100ul/200ul
    ￥1180 - 2800