Sample:
Du145(Human) Cell Lysate at 30 ug
Primary: Anti-PTPLB (bs-4429R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 28 kD
Observed band size: 28 kD
- ￥1180 - 2800
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- bs-4429R
- 2025年10月24日
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产品信息以Bioss网站为准
- 规格：
50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-4429R |
| 英文名称 | PTPLB Rabbit pAb |
| 中文名称 | 蛋白酪氨酸磷酸酶PTPLB抗体 |
| 英文别名 | 3 hydroxyacyl CoA dehydratase 2; 3-hydroxyacyl-CoA dehydratase 2; HACD2; HACD2_HUMAN; Protein tyrosine phosphatase like(proline instead of catalytic arginine) member b; Protein tyrosine phosphatase like member B; Protein-tyrosine phosphatase-like member B; PTPLB; Very-long-chain(3R)-3-hydroxyacyl-[acyl-carrier protein] dehydratase 2. |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 Not yet tested in other applications. |
| 交叉反应 | Human, Mouse |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human PTPLB |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 28 kDa |
| 浓度 | 1mg/ml |
| 储存液 | Preservative: 0.02% Proclin300, Constituents: 1% BSA, 0.01M PBS, pH7.4. |
| 研究领域 | Cardiovascular > Lipids / Lipoproteins > Fatty Acids > Synthesis Metabolism > Pathways and Processes > Redox metabolism > Fatty acid oxidation |
| 亚细胞定位 | Endoplasmic reticulum membrane. |
| 组织特异性 | Highly expressed in testis, spleen, prostate, colon and heart, followed by moderate expression in thymus, ovary, small intestine, peripheral blood leukocytes, liver, skeletal muscle and pancreas. Weakly detected in kidney, placenta, brain and lung. |
| 相似性 | Belongs to the very long-chain fatty acids dehydratase HACD family. |
| 功能 | Responsible for the dehydration step in very long-chain fatty acids (VLCFAs) synthesis. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | The protein encoded by this gene can catalyze the third step (dehydration) in the conversion of long chain fatty acids to very long chain fatty acids. The encoded protein localizes to the endoplasmic reticulum membrane. [provided by RefSeq, Jul 2016] |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
| {IF} | {1:100-500} |
Sample:
LOVO(Human) Cell Lysate at 30 ug
Primary: Anti-PTPLB (bs-4429R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 28 kD
Observed band size: 28 kD
LOVO(Human) Cell Lysate at 30 ug
Primary: Anti-PTPLB (bs-4429R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 28 kD
Observed band size: 28 kD
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PTPLB) Polyclonal Antibody, Unconjugated (bs-4429R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
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PTPLB Rabbit pAb(bs-4429R)-50ul/100ul/200ul
￥1180 - 2800
