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- bs-1583R
- 2025年10月16日
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| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-1583R |
| 英文名称 | SFTPD Rabbit pAb |
| 中文名称 | 肺表面活性蛋白D抗体 |
| 英文别名 | SFTPD surfactant protein D; pulmonary surfactant-associated glycoprotein D; COLEC 7; COLEC7; Collectin7; PSP D; PSPD; Pulmonary surfactant apoprotein; Pulmonary surfactant associated protein D; SFTP 4; SFTP4; SFTPD; SP D; Surfactant associated protein pulmonary 4; Surfactant pulmonary associated protein D; SFTPD_HUMAN; Pulmonary surfactant-associated protein D; PSP-D; SP-D; Collectin-7; Lung surfactant protein D. |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=2ug/Test Not yet tested in other applications. |
| 交叉反应 | Human, Mouse (Rat) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human SP-D |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 41 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 亚基 | Oligomeric complex of 4 set of homotrimers. |
| 亚细胞定位 | Secreted, extracellular space, extracellular matrix. Secreted, extracellular space, surface film. |
| 翻译后修饰 | The N-terminus is blocked.
Hydroxylation on proline residues within the sequence motif, GXPG, is most likely to be 4-hydroxy as this fits the requirement for 4-hydroxylation in vertebrates. |
| 相似性 | Belongs to the SFTPD family.
Contains 1 C-type lectin domain. Contains 1 collagen-like domain. |
| 功能 | Contributes to the lung's defense against inhaled microorganisms. May participate in the extracellular reorganization or turnover of pulmonary surfactant. Binds strongly maltose residues and to a lesser extent other alpha-glucosyl moieties. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | Surfactant protein D contributes to the lung's defense against inhaled microorganisms. It's a calcium ion dependent carbohydrate binding protein and is structurally similar to other C type mammalian lectins, such as conglutinin and SPA. It has a molecular size of approximately 37-43kDa in its reduced state, 620kDa in non dissociating conditions. Surfactant protein D enhances the production of oxygen radicals by rat alveolar macrophages and regulates some actions of SPA, which is the most abundant surfactant protein. Surfactant protein D is synthesized and secreted by alveolar epithelial type II cells. |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
| {IF} | {1:100-500} |
| {Flow-Cyt} | {2ug/Test} |
Primary Antibody (green line): Rabbit Anti-SFTPD antibody (bs-1583R)
Dilution: 2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-FITC
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
Primary Antibody (green line): Rabbit Anti-SFTPD antibody (bs-1583R)
Dilution: 2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-FITC
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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文献和实验[IF={{ 4.92 }}] {Ferretti, Andrew, et al. "Autoantibody-Mediated Pulmonary Alveolar Proteinosis in Rasgrp1-Deficient Mice." The Journal of Immunology (2016): 1502248.} {IHC} {="Mouse"}
[IF={{ 4.06 }}] {Malvin NP et al. Autophagy proteins are required for club cell structure and function in airways. Am J Physiol Lung Cell Mol Physiol. 2019 May 22.} {IHC} {Mouse&Human}
[IF={{ 3.419 }}] {Shota Mitsuboshi. et al. A novel alveolar epithelial cell sheet fabricated under feeder-free conditions for potential use in pulmonary regenerative therapy. Regen Ther. 2022 Mar;19:113} {IHC} {Rat}
[IF={{ 2.94 }}] {Sung, Hsin-Ching, et al. "The protective effect of eupafolin against TNF-α-induced lung inflammation via the reduction of intercellular cell adhesion molecule-1expression." Journal of Ethnopharmacology (2015).} {IHC} {="Mouse"}
[IF={{ 1.19 }}] {Furukawa et al. Databases for technical aspects of immunohistochemistry. (2017) J.Toxicol.Patho. 30:79-107} {IHC} {Mouse}
= NAL), appears to form upon oxidative cyclization of the nonfluorescent 2:1 lysine-HNE Michael adduct-Schiff base cross-link (Scheme 1). Polyclonal antibody (PAb) to the NAL-HNE fluorophore was raised in rabbit and found to be highly specific
48T/96T 1300/2300 进口分装 F1581 ★ 大鼠白介素-1 b (rat IL-1 b ) ELISA 48T/96T 1300/2300 进口分装 F1582 ★ 大鼠白介素-2 (rat IL-2) ELISA 48T/96T 1300/2300 进口分装 F1583 大鼠白介素-2 受体 (rat IL-2R) ELISA 48T/96T 1800/2800 进口分装 F1584 大鼠白介素-3(rat IL
48T/96T 1300/2300 进口分装 F1581 ★ 大鼠白介素-1 b (rat IL-1 b ) ELISA 48T/96T 1300/2300 进口分装 F1582 ★ 大鼠白介素-2 (rat IL-2) ELISA 48T/96T 1300/2300 进口分装 F1583 大鼠白介素-2 受体 (rat IL-2R) ELISA 48T/96T 1800/2800 进口分装 F1584 大鼠白介素-3(rat IL
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