Sample:
_x000D_
Kidney (Mouse) Lysate at 40 ug
_x000D_
Primary: Anti-ZDHHC6 (bs-18485R) at 1/300 dilution
_x000D_
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
_x000D_
Predicted band size: 48 kD
_x000D_
Observed band size: 48 kD
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- 技术资料
- 规格:
50ul/100ul/200ul
| 规格: | 50ul | 产品价格: | ¥1180.0 |
|---|---|---|---|
| 规格: | 100ul | 产品价格: | ¥1980.0 |
| 规格: | 200ul | 产品价格: | ¥2800.0 |
| 产品编号 | bs-18485R |
| 英文名称 | ZDHHC6 Rabbit pAb |
| 中文名称 | ZDHHC6蛋白抗体 |
| 英文别名 | DHHC-6; FLJ21952; Probable palmitoyltransferase ZDHHC6; Transmembrane protein H4; ZDHC6_HUMAN; Zdhhc6; Zinc finger DHHC domain-containing protein 6; Zinc finger protein 376; ZNF376. |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 Not yet tested in other applications. |
| 交叉反应 | Human, Mouse, Rat (Pig, Horse) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human ZDHHC6 |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 48 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 亚细胞定位 | Membrane. |
| 相似性 | Belongs to the DHHC palmitoyltransferase family. _x000D_ Contains 1 DHHC-type zinc finger. |
| 功能 | Palmitoylates calnexin (CALX), which is required for its association with the ribosome-translocon complex and efficient folding of glycosylated proteins. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
| {IF} | {1:100-500} |


Sample:
_x000D_ Lane 1: Mouse Pancreas tissue lysates
_x000D_ Lane 2: Mouse Cerebrum tissue lysates
_x000D_ Lane 3: Mouse Testis tissue lysates
_x000D_ Lane 4: Mouse Raw264.7 cell lysates
_x000D_ Lane 5: Mouse NIH/3T3 cell lysates
_x000D_ Lane 6: Rat Kidney tissue lysates
_x000D_ Lane 7: Rat Cerebrum tissue lysates
_x000D_ Lane 8: Rat Testis tissue lysates
_x000D_ Lane 9: Human HL-60 cell lysates
_x000D_ Primary: Anti-ZDHHC6 (bs-18485R) at 1/1000 dilution
_x000D_ Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
_x000D_ Predicted band size: 48 kDa
_x000D_ Observed band size: 50 kDa
_x000D_ Lane 1: Mouse Pancreas tissue lysates
_x000D_ Lane 2: Mouse Cerebrum tissue lysates
_x000D_ Lane 3: Mouse Testis tissue lysates
_x000D_ Lane 4: Mouse Raw264.7 cell lysates
_x000D_ Lane 5: Mouse NIH/3T3 cell lysates
_x000D_ Lane 6: Rat Kidney tissue lysates
_x000D_ Lane 7: Rat Cerebrum tissue lysates
_x000D_ Lane 8: Rat Testis tissue lysates
_x000D_ Lane 9: Human HL-60 cell lysates
_x000D_ Primary: Anti-ZDHHC6 (bs-18485R) at 1/1000 dilution
_x000D_ Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
_x000D_ Predicted band size: 48 kDa
_x000D_ Observed band size: 50 kDa

Paraformaldehyde-fixed, paraffin embedded (rat placenta); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (ZDHHC6) Polyclonal Antibody, Unconjugated (bs-18485R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (mouse placenta); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (ZDHHC6) Polyclonal Antibody, Unconjugated (bs-18485R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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ZDHHC6 | DHHC-6 Rabbit pAb(bs-18485R)-50ul/100ul/200ul
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