Protein: mouse brain lysates at 40ug;
Primary: rabbit Anti-LC3 (bs-8878R) at 1:300;
Secondary: HRP conjugated Goat-Anti-rabbit IgG(bs-0295G-HRP) at 1: 5000;
Predicted band size:14 kD Observed band size:14,16 kD
- ￥1180 - 2800
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- bs-8878R
- 2025年10月24日
- 产品信息以Bioss网站为准
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50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-8878R |
| 英文名称 | LC3 Rabbit pAb |
| 中文名称 | 自噬微管相关蛋白轻链3抗体 |
| 英文别名 | Autophagy related protein LC3 A; Autophagy related protein LC3 B; Autophagy related ubiquitin like modifier LC3 A; Autophagy related ubiquitin like modifier LC3 B; MAP1 light chain 3 like protein 1; MAP1 light chain 3 like protein 2; MAP1A/1B light chain 3 A; MAP1A/1B light chain 3 B; MAP1A/1BLC3; MAP1A/MAP1B LC3 A; MAP1A/MAP1B LC3 B; MAP1ALC3; MAP1BLC3; MAP1LC3A; MAP1LC3B; Microtubule associated protein 1 light chain 3 alpha; Microtubule associated protein 1 light chain 3 beta; Microtubule associated proteins 1A/1B light chain 3; Microtubule associated proteins 1A/1B light chain 3A; Microtubule associated proteins 1A/1B light chain 3B; MLP3A_HUMAN. |
| 产品应用 | WB=1:500-2000 Not yet tested in other applications. |
| 交叉反应 | Mouse (Human, Rat, Pig, Cow, Horse) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human LC3 |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 14/16 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 亚基 | 3 different light chains, LC1, LC2 and LC3, can associate with MAP1A and MAP1B proteins. Interacts with SQSTM1. Interacts with TP53INP1 and TP53INP2 |
| 亚细胞定位 | Cytoplasm, cytoskeleton. Endomembrane system; Lipid-anchor. Cytoplasmic vesicle, autophagosome membrane; Lipid-anchor. Cytoplasmic vesicle, autophagosome. Note=LC3-II binds to the autophagic membranes. |
| 组织特异性 | Most abundant in heart, brain, liver, skeletal muscle and testis but absent in thymus and peripheral blood leukocytes. |
| 翻译后修饰 | The precursor molecule is cleaved by APG4B/ATG4B to form the cytosolic form, LC3-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form the membrane-bound form, LC3-II. |
| 相似性 | Detects a band of approximately 16 kDa (predicted molecular weight: 14 kDa). |
| 功能 | Ubiquitin-like modifier involved in formation of autophagosomal vacuoles (autophagosomes) |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
Sample:
Lane 1: Cerebrum (Mouse) Lysate at 40 ug
Lane 2: Cerebellum (Mouse) Lysate at 40 ug
Lane 3: Testis (Mouse) Lysate at 40 ug
Primary: Anti-LC3 (bs-8878R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 17/14 kD
Observed band size: 17 kD
Lane 1: Cerebrum (Mouse) Lysate at 40 ug
Lane 2: Cerebellum (Mouse) Lysate at 40 ug
Lane 3: Testis (Mouse) Lysate at 40 ug
Primary: Anti-LC3 (bs-8878R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 17/14 kD
Observed band size: 17 kD
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文献和实验该产品被引用文献
[IF={{ 7.31 }}] {Yi-Jing Yang. et al. Lycium barbarum Polysaccharides Regulating miR-181/Bcl-2 Decreased Autophagy of Retinal Pigment Epithelium with Oxidative Stress. OXID MED CELL LONGEV. 2023 Jan 05;2023:9554457} {WB} {Mouse}
[IF={{ 7 }}] {Li Hongli. et al. Linc00707 regulates autophagy and promotes the progression of triple negative breast cancer by activation of PI3K/AKT/mTOR pathway. CELL DEATH DISCOV. 2024 Mar;10(1):1-14} {IF,WB} {Human}
[IF={{ 6.814 }}] {Guo-Jian Jiang. et al. Ultraviolet B irradiation induces senescence of human corneal endothelial cells in vitro by DNA damage response and oxidative stress. J PHOTOCH PHOTOBIO B. 2022 Oct;235:112568} {WB} {Human}
[IF={{ 6.551 }}] {Yukun Fang. et al. Activation of the ROS/HO-1/NQO1 signaling pathway contributes to the copper-induced oxidative stress and autophagy in duck renal tubular epithelial cells. Sci Total Environ. 2021 Feb;757:143753} {WB} {Duck}
[IF={{ 5.811 }}] {Lang Xiu-Yuan. et al. Coeloglossum viride Var. Bracteatum Extract Attenuates MPTP-Induced Neurotoxicity in vivo by Restoring BDNF-TrkB and FGF2-Akt Signaling Axis and Inhibiting RIP1-Driven Inflammation. FRONT PHARMACOL. 2022 Apr;0:1571} {WB} {Mouse}
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LC3 Rabbit pAb(bs-8878R)-50ul/100ul/200ul
￥1180 - 2800
