PKA C alpha + beta Rabbit pAb(bs-2371R)-50ul/100ul/200ul

PKA C alpha + beta Rabbit pAb(

bs-2371R)-50ul/100ul/200ul
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  • ￥1180 - 2800
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  • bs-2371R
  • 2025年10月24日
  • 产品信息以Bioss网站为准
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北京博奥森生物技术有限公司
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      50ul/100ul/200ul

    规格：50ul产品价格：￥1180.0
    规格：100ul产品价格：￥1980.0
    规格：200ul产品价格：￥2800.0
    产品编号bs-2371R
    英文名称PKA C alpha + beta Rabbit pAb
    中文名称蛋白激酶A催化亚基抗体
    英文别名PKA C alpha+beta; PKA C alpha/beta; PKA C beta; PKACB; KAPCB_HUMAN; PRKACB; Protein kinase cAMP dependent catalytic beta; KAPCA_HUMAN; cAMP-dependent protein kinase catalytic subunit alpha; PKA C-alpha.
    产品应用WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500

    Not yet tested in other applications.
    Optimal working dilutions must be determined by the end user.

    交叉反应Human, Mouse, Rat (Chicken, Dog, Pig, Cow)
    抗体来源Rabbit
    免疫原KLH conjugated synthetic peptide derived from human PKA beta
    亚型IgG
    性状Liquid
    纯化方法affinity purified by Protein A
    克隆类型Polyclonal
    理论分子量40/50 kDa
    浓度1mg/ml
    储存液0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
    研究领域

    Cancer > Cancer Metabolism > Metabolic signaling pathway > Integration of energy metabolism

    Cancer > Cancer Metabolism > Metabolic signaling pathway > Metabolism of lipids and lipoproteins

    Cancer > Signal transduction > Protein phosphorylation > Serine/threonine kinases

    Metabolism > Pathways and Processes > Metabolic signaling pathways > Energy transfer pathways > Integration of energy

    Metabolism > Pathways and Processes > Metabolic signaling pathways > Lipid and lipoprotein metabolism > Lipid metabolism

    Metabolism > Types of disease > Cancer

    Neuroscience > Neurotransmission > Intracellular Signaling > Kinases

    Signal Transduction > Protein Phosphorylation > Ser / Thr Kinases > PKA

    Signal Transduction > Protein Phosphorylation > Ser / Thr Kinases > PKC

    亚基A number of inactive tetrameric holoenzymes are produced by the combination of homo- or heterodimers of the different regulatory subunits associated with two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. The cAMP-dependent protein kinase catalytic subunit binds PJA2 (By similarity).
    亚细胞定位oplasm. Cell membrane. Nucleus (By similarity). Note=Translocates into the nucleus (monomeric catalytic subunit) (By similarity). The inactive holoenzyme is found in the cytoplasm (By similarity).
    组织特异性Isoform 1 is most abundant in the brain, with low level expression in kidney. Isoform 2 is predominantly expressed in thymus, spleen and kidney. Isoform 3 and isoform 4 are only expressed in the brain.
    翻译后修饰Asn-3 is partially deaminated to Asp giving rise to 2 major isoelectric variants, called CB and CA respectively (By similarity).
    相似性Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. cAMP subfamily.
    Contains 1 AGC-kinase C-terminal domain.
    Contains 1 protein kinase domain.
    功能Mediates cAMP-dependent signaling triggered by receptor binding to GPCRs. PKA activation regulates diverse cellular processes such as cell proliferation, the cell cycle, differentiation and regulation of microtubule dynamics, chromatin condensation and decondensation, nuclear envelope disassembly and reassembly, as well as regulation of intracellular transport mechanisms and ion flux. Regulates the abundance of compartmentalized pools of its regulatory subunits through phosphorylation of PJA2 which binds and ubiquitinates these subunits, leading to their subsequent proteolysis.
    保存条件Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
    注意事项This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
    背景资料cAMP is a signaling molecule important for a variety of cellular functions. cAMP exerts its effects by activating the cAMP dependent protein kinase (AMPK), which transduces the signal through phosphorylation of different target proteins. The inactive holoenzyme of AMPK is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits of AMPK have been identified in humans. PKA beta (catalytic subunit) is a member of the Ser/Thr protein kinase family and is a catalytic subunit of AMPK.

     

    应用推荐稀释比例
    {WB}{1:500-2000}
    {IHC-P}{1:100-500}
    {IHC-F}{1:100-500}
    {IF}{1:100-500}

     

    PKA C alpha + beta Rabbit pAb(
    P‌‌araformaldehyde-fixed, paraffin embedded (Human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PKA C alpha+beta) Polyclonal Antibody, Unconjugated (bs-2371R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
    PKA C alpha + beta Rabbit pAb(
    Sample:
    Brain(Rat) Lysate at 40 ug
    Primary: Anti-PKA C alpha+beta (bs-2371R) at 1/300 dilution
    Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
    Predicted band size: 40/50 kD
    Observed band size: 50 kD
    PKA C alpha + beta Rabbit pAb(
    Sample:
    Testis (Mouse) Lysate at 40 ug
    Testis(Rat) Lysate at 40 ug
    Primary: Anti-PKA C alpha+beta (bs-2371R) at 1/300 dilution
    Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
    Predicted band size: 40/50 kD
    Observed band size: 50 kD
    PKA C alpha + beta Rabbit pAb(
    Sample:
    Lane 1: Mouse Testis tissue lysates
    Lane 2: Mouse Cerebrum tissue lysates
    Lane 3: Mouse Heart tissue lysates
    Lane 4: Rat Heart tissue lysates
    Lane 5: Human MCF-7 cell lysates
    Lane 6: Human Hela cell lysates
    Lane 7: Human Huvec cell lysates
    Primary: Anti- PKA C alpha + beta (bs-2371R) at 1/1000 dilution
    Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
    Predicted band size: 40/50kD kDa
    Observed band size: 50kD kDa

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