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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
常温
- 保质期:
三年
- 英文名:
pRL-null
- 库存:
60
- 供应商:
信裕生物
- 规格:
5ug质粒
基本信息
| 质粒类型: | 启动子报告载体 |
|---|---|
| 载体大小: | 3320 bp (查看载体序列) |
| 载体抗性: | Ampicillin (氨苄青霉素) |
订购信息
| 产品编号 | 产品名称 | 规格 | 价格 |
|---|---|---|---|
| XY1567 | pRL-null | 5ug质粒 |
¥1000.00 |
质粒图谱
载体描述
The pRL-null Vector(a,b) (Figure 1) is intended for use in constructing a control reporter vector that may be used in combination with any experimental reporter vector to cotransfect mammalian cells. All of Promega’s pRL Reporter Vectors contain a cDNA(b) (Rluc) encoding Renilla luciferase, which was originally cloned from the marine organism Renilla reniformis (sea pansy; 1). As described below, the Renilla luciferase cDNA contained within the pRL Vectors has been modified slightly to provide greater utility.
The pRL-null Vector contains no enhancer or promoter elements. Rather, it contains a multiple cloning region upstream of Rluc to allow for the cloning of any desired regulatory element(s) to drive expression of Renilla luciferase. Renilla luciferase is a 36kDa monomeric protein that does not require post-translational modification for activity (2). Therefore, like firefly luciferase, the enzyme may function as a genetic reporter immediately following translation. For information about the use of this plasmid in conjunction with a reporter vector containing the firefly luciferase gene, refer to the Dual-Luciferase® Reporter Assay System(c,d) Technical Manual (#TM040).
The pRL Vectors are isolated from a dam–/dcm– E. coli K host strain, allowing digestion with restriction enzymes that are sensitive to dam and dcm methylation.
The GenBank®/EMBL Accession Number for the pRL-null Vector is AF025844.
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文献和实验细胞类型中,可高强度、组成型表达Rluc。在转基因小鼠中,CMV增强子/启动子具有泛主特点,在检测的28种小鼠组织中,24种有转录活力。pRL-Null载体pRL-null无真核启动子和增强子序列,在Rluc上游有一些单一酶切位点,可提供最大灵活性来克隆所需的启动子序列,启动海洋腔肠荧光素酶的表达。图9所显示的pRL-TK载体代表了pRL载体所共有的特点,在每个pRL载体的指定启动子的紧下游是一个嵌合的内含子,可提供海洋腔肠荧光素酶的增强表达。内含子由人β-珠蛋白质内含子1的5-供体剪切位点和免疫
in the retrieval of morphine withdrawal memory. [腺相关病毒, 成瘾, AAV1, AAV-WGA-Cre] 注射部位:小鼠PrL载体:AAV-hSyn-mCherry-IRES-WGA-Cre病毒滴度:4.28 × 1012 VG/mL注射体积:300nl观察时间:4周
3basic amp PGL3-control PGL3control amp PGL3-enhancer amp PGL3-promoter amp pRL-TK pRLTK amp pRL-CMV pRLCMV amp pRL-SV40 pRLSV40 amp pRL家族海肾荧光素酶(Rluc)对照报告基因载体系列是设计应用于双荧光素酶报告基因检测系统的。pRL载体提供组成性表达的海肾荧光素酶,可与一个萤火虫荧光素酶载体联合共转染哺乳动物细胞。海肾荧
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