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- 详细信息
- 技术资料
- 保存条件:
-20℃
- 保质期:
半年
- 英文名:
pRL-CMV
- 库存:
200
- 供应商:
沪震生物
- 规格:
5μl
pRL-CMV
产品信息
| 产品货号 | 产品名称 | 产品规格 | 优惠价 |
|---|---|---|---|
| HZ0196 | pRL-CMV | 20μl |
¥请询价 |
使用说明
沪震质粒平台的各批次质粒菌株发货前均经过严格的多重验证,如存在质量问题,请在收到产品的三个月内通知我司。收到质粒后请短暂离心,取2μl转化至对应感受态中,挑取单克隆重新提取质粒后使用。
基本信息
| 启动子: | CMV,T7 |
|---|---|
| 复制子: | pUC ori |
| 终止子: | SV40 poly(A) signal |
| 质粒分类: | 哺乳细胞,信号通路报告载体 |
| 质粒大小: | 4079bp |
| 原核抗性: | Amp |
| 克隆菌株: | DH5α |
| 培养条件: | 37℃,有氧 LB |
| 表达宿主: | 哺乳细胞 |
| 诱导方式: | 无须诱导,瞬时表达 |
| 5'测序引物: | CMV-F:CGCAAATGGGCGGTAGGCGTG |
| 3'测序引物: | Sv40-polyA-R:GAAATTTGTGATGCTATTGC |
质粒简介
The pRL-CMV Vector may be used in combination with any experimental reporter vector to co-transfect mammalian cells. However, it is important to realize that trans effects between promoters on co-transfected plasmids can potentially affect reporter gene expression . Primarily, this is of concern when either the control or experimental reporter vector, or both, contain very strong promoter/enhancer elements (such as CMV). The occurrence and magnitude of such effects will depend on several factors: a) the combination and activities of the genetic regulatory elements present on the co-transfected vectors, b) the relative ratio of experimental vector to control vector introduced into the cells, and c) the cell type transfected.
质粒图谱
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载体pRL-CMV
¥800 - 1380









