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文献和实验Chromatin immunoprecipitation on native chromatin from cells and tissues
fraction P, in 50µl of dialysis buffer and store at 4°C. Quality Control of Chromatin Take the Optical Density (OD) of each fraction at 260nm; In separate 1.5mL Eppendorf tubes, put 0.5µg of each fraction (S1, S2, and P);
A/B/C Random Amplification Protocol
DNA. Round B PCR 30 µL Round A DNA product 20 µL 10X Thermo Pol PCR Buffer 2 µL 25 mM dNTP 4 µL 100 µM Primer B Cy5 (sample) or Cy3 (reference DNA) 2 µL Ampli Taq 0.1 µL Pfu Turbo 142 µL dH 2 O
Sample Preparation for Microinjection
Buffers, e.g., PB’s, are not recommended as this often leads to blockages of the capillaries. DNA concentrations of 20 µg/ml to 200 µg/ml for plasmid DNA injection has been recommended [1] . Storage Store at �20°C in small aliquots of 5�10 µl
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