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文献和实验Rapid Extraction of High Quality DNA from Whole Blood Stored at 4ºC for Long Period
should be shake gently by rotating blood mixer (vortex) Pour 500 µl of blood into a 1.5 ml eppendorf tube and add 1000 µl of red cell lysis buffer. Shake microfuge tube gently (up to homogenizing), then spin for 2 minutes at 7000 rpm. Discard
The ribonuclease protection assay (RPA)
, 5X transcription buffer, and RPA template set to RT. For each probe synthesis, add the following in order to a 1.5 ml Eppendorf tube: 1 µl RNasin® 1 µl GACU pool 2 µl DTT 4 µl 5X transcription buffer 1 µl RPA Template Set 10 µl [a-32
Lipoprotein Isolation - First week
with dist. water. 8. Leave an aliquot (300 µl) of every other fraction in labeled modified Eppendorf tubes for dialysis. 9. Dialyze against ~1 l of dist. water in the cold room, changing the buffer once. 10. Carefully transfer
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