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- ZYMO RESEARCH
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- 2026年01月13日
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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 英文名：
ZymoBIOMICS DNA Miniprep Kit
- 供应商：
简石生物
HIGHLIGHTS
- Validated Unbiased for Microbiome Measurements: Unbiased cellular lysis validated using the ZymoBIOMICS Microbial Community Standard.
- Inhibitor-Free DNA from Any Sample: Isolate ultra-pure DNA ready for any downstream application.
- Certified Low Bioburden: Boost your detection limit for low abundance microbes.
DESCRIPTION
The ZymoBIOMICS DNA Kits are microbial DNA purification kits designed for purifying DNA from a variety of sample inputs that is immediately ready for microbiome or metagenome analyses. The ZymoBIOMICS lysis system eliminates bias associated with unequal lysis efficiencies of different organisms (e.g. Gram-negative/positive bacteria, fungus, protozoans, and algae) making it ideal for microbial community profiling. Uniform mechanical lysis of all microbes is achieved by bead beating with the innovative ultra-high density BashingBeads. This kit is equipped with our OneStep PCR Inhibitor removal technology, enabling PCR amplification from DNA derived from inhibitor-rich environmental samples. Purified DNA is ideal for all downstream applications including PCR, arrays, 16S rRNA gene sequencing, and shotgun sequencing. DNA Size is 15-20 kb.
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文献和实验Yeast boiling DNA miniprep (Robzyk & Kassir, Nucl. Acid. Res. 20: 3790, 1992) Harvest 1.5 ml of an ON yeast culture grown under selective conditions (5K, 5 minutes). Resuspend in 100 µl STET (8% sucrose, 50 mM Tris pH 8, 50 mM EDTA, 5% Triton X
of 5 M potassium acetate solution (pH 4.8) . This solution neutralizes NaOH in the previous lysis step while precipitating the genomic DNA and SDS in an insoluble white, rubbery precipitate. Spin at top speed 1 min. 6. Transfer supernatant to new
Simple Miniprep DNA Preparation
SNGE,NIMH,NIH http://intramural.nimh.nih.gov/lcmr/snge/Protocols/Miniprep.html SOLUTIONS: •Resuspension buffer: 25mM Tris-HCl (ph8) 50mM Na2 EDTA 1% glucose Autoclave and store at 4℃. •NaOH/SDS: 0.2 N NaOH/1% SDS. Prepare fresh
