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- 2025年12月31日
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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 英文名：
Quick-DNA Miniprep Plus Kit
- 供应商：
简石生物
HIGHLIGHTS
- Quick & Easy: Simple 20 minute procedure.
- Highest Yield: Recover 3x more DNA.
- Ultra-Pure: Ready for qPCR, Next-Gen Sequencing, arrays, etc.
DESCRIPTION
The Quick-DNA Plus Kits are the easiest DNA isolation kits for high yield total DNA extraction (e.g., genomic, mitochondrial, viral) from cell culture, solid tissue, saliva, and any biological fluid sample. Innovative reagents and Zymo-Spin Technology allow for ultra-pure and concentrated genomic DNA (> 50 kb) to be eluted in as little as 35 µl. Zymo-Spin Columns ensure no buffer retention. Purified DNA is RNA-free,removing the need for RNase A treatments and ensuring accurate quantification for applications like library preparations. Isolated DNA is suitable for immediate use in sensitive downstream applications including qPCR, DNA-seq, arrays, and methylation analysis.
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文献和实验Yeast boiling DNA miniprep (Robzyk & Kassir, Nucl. Acid. Res. 20: 3790, 1992) Harvest 1.5 ml of an ON yeast culture grown under selective conditions (5K, 5 minutes). Resuspend in 100 µl STET (8% sucrose, 50 mM Tris pH 8, 50 mM EDTA, 5% Triton X
of 5 M potassium acetate solution (pH 4.8) . This solution neutralizes NaOH in the previous lysis step while precipitating the genomic DNA and SDS in an insoluble white, rubbery precipitate. Spin at top speed 1 min. 6. Transfer supernatant to new
Simple Miniprep DNA Preparation
SNGE,NIMH,NIH http://intramural.nimh.nih.gov/lcmr/snge/Protocols/Miniprep.html SOLUTIONS: •Resuspension buffer: 25mM Tris-HCl (ph8) 50mM Na2 EDTA 1% glucose Autoclave and store at 4℃. •NaOH/SDS: 0.2 N NaOH/1% SDS. Prepare fresh
