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- 2026年01月15日
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文献和实验(2). Two of these controls are: A negative control siRNA with the same nucleotide composition as your siRNA but which lacks significant sequence homology to the genome . To design a negative control siRNA, scramble the nucleotide sequence of the gene-specific
of controls to ensure the validity of the data. The editors of Nature Cell Biology have recommended several controls (2). Two of these controls are: A negative control siRNA with the same nucleotide composition as your siRNA but which lacks significant
siRNA sequence. The control RNA should have the same length and nucleotide composition as the siRNA but have at least 4-5 bases mismatched to the siRNA. Make sure the scrambling will not create new homology to other genes. Tom Tuschl's rules
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