- 询价
- Cell Signaling Technology
- 9649
- USA
- 2026年05月28日
- western blot,免疫组化（IHC）,免疫荧光（IF）,流式细胞（Flow Cyt）,染色质免疫沉淀分析（ChIP）
- 兔
- 人,小鼠,大鼠,驴,斑马鱼,酵母菌
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- 文献和实验
- 技术资料
- 抗体英文名：
Acetyl-Histone H3 (Lys9) (C5B11) Rabbit mAb
- 抗原：
/
- 应用范围：
western blot,免疫组化（IHC）,免疫荧光（IF）,流式细胞（Flow Cyt）,染色质免疫沉淀分析（ChIP）
- 宿主：
兔
- 适应物种：
人,小鼠,大鼠,驴,斑马鱼,酵母菌
- 级别：
详见MSDS文件
- 库存：
大量
- 抗原来源：
/
- 保质期：
详见说明书
- 供应商：
CST
- 是否单克隆：
1
- 保存条件：
-20°c
- 规格：
40 ul (4 western blots)/100 ul (10 western blots)/<a href="http://www.cellsignal.com/ddt/custom_reagents.html" target="_blank">carrier free & custom formulation / quantity</a>
| 规格： | 产品价格： | ￥请询价 | |
|---|---|---|---|
| 规格： | 40 ul (4 western blots) | 产品价格： | ￥请询价 |
| 规格： | 100 ul (10 western blots) | 产品价格： | ￥请询价 |
| 规格： | <a href="http://www.cellsignal.com/ddt/custom_reagents.html" target="_blank">carrier free & custom formulation / quantity</a> | 产品价格： | ￥请询价 |
Product Pathways - Chromatin Regulation / Epigenetics
Acetyl-Histone H3 (Lys9) (C5B11) Rabbit mAb #9649
PhosphoSitePlus ® protein, site, and accession data: H3
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W IHC-P IF-IC F ChIP | H M R Mk Z (Sc) | Endogenous | 17 | Rabbit IgG |
Applications Key: W=Western Blotting IHC-P=Immunohistochemistry (Paraffin) IF-IC=Immunofluorescence (Immunocytochemistry) F=Flow Cytometry ChIP=Chromatin IP
Reactivity Key: H=Human M=Mouse R=Rat Mk=Monkey Z=Zebrafish Sc=S. cerevisiae
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
- 9649:
- ChIP Agarose , ChIP Magnetic , Flow , IHC / Paraffin , Immunofluorescence , Immunoprecipitation , Western Blotting
Specificity / Sensitivity
Acetyl-Histone H3 (Lys9) (C5B11) Rabbit mAb detects endogenous levels of histone H3 only when acetylated on Lys9. This antibody does not cross-react with other acetylated histones.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the amino terminus of histone H3 in which Lys9 is acetylated.
Western Blotting
Western blot analysis of lysates from HeLa and NIH/3T3 cells, untreated or TSA-treated (400 nM for 18 hours) using Acetyl-Histone H3 (Lys9) (C5B11) Rabbit mAb.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human osteosarcoma using Acetyl-Histone H3 (Lys 9) (C5B11) Rabbit mAb.
Flow Cytometry
Flow cytometric analysis of untreated HeLa cells using Acetyl-Histone H3 (Lys9) (C5B11) Rabbit mAb #9649 versus propidium iodide (DNA content). Note positive staining in cycling cells (box).
IF-IC
Confocal immunofluorescent analysis of HeLa cells, untreated (left) or treated with TSA #9950 (right), using Acetyl-Histone H3 (Lys9) (C5B11) Rabbit mAb (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red).
Chromatin IP
Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 HeLa cells and either 10 μl of Acetyl-Histone H3 (Lys9) (C5B11) Rabbit mAb or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using SimpleChIP® Human RPL30 Exon 3 Primers #7014, SimpleChIP® Human GAPDH Exon 1 Primers #5516, SimpleChIP® Human MyoD1 Exon 1 Primers #4490, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.
Background
Modulation of chromatin structure plays an important role in the regulation of transcription in eukaryotes. The nucleosome, made up of DNA wound around eight core histone proteins (two each of H2A, H2B, H3, and H4), is the primary building block of chromatin (1). The amino-terminal tails of core histones undergo various post-translational modifications, including acetylation, phosphorylation, methylation, and ubiquitination (2-5). These modifications occur in response to various stimuli and have a direct effect on the accessibility of chromatin to transcription factors and, therefore, gene expression (6). In most species, histone H2B is primarily acetylated at Lys5, 12, 15, and 20 (4,7). Histone H3 is primarily acetylated at Lys9, 14, 18, 23, 27, and 56. Acetylation of H3 at Lys9 appears to have a dominant role in histone deposition and chromatin assembly in some organisms (2,3). Phosphorylation at Ser10, Ser28, and Thr11 of histone H3 is tightly correlated with chromosome condensation during both mitosis and meiosis (8-10). Phosphorylation at Thr3 of histone H3 is highly conserved among many species and is catalyzed by the kinase haspin. Immunostaining with phospho-specific antibodies in mammalian cells reveals mitotic phosphorylation at Thr3 of H3 in prophase and its dephosphorylation during anaphase (11).
- Workman, J.L. and Kingston, R.E. (1998) Annu Rev Biochem 67, 545-79.
- Hansen, J.C. et al. (1998) Biochemistry 37, 17637-41.
- Strahl, B.D. and Allis, C.D. (2000) Nature 403, 41-5.
- Cheung, P. et al. (2000) Cell 103, 263-71.
- Bernstein, B.E. and Schreiber, S.L. (2002) Chem Biol 9, 1167-73.
- Jaskelioff, M. and Peterson, C.L. (2003) Nat Cell Biol 5, 395-9.
- Thorne, A.W. et al. (1990) Eur J Biochem 193, 701-13.
- Hendzel, M.J. et al. (1997) Chromosoma 106, 348-60.
- Goto, H. et al. (1999) J Biol Chem 274, 25543-9.
- Preuss, U. et al. (2003) Nucleic Acids Res 31, 878-85.
- Dai, J. et al. (2005) Genes Dev 19, 472-88.
Application References
- Kong, D.K. et al. (2010) Mol Biol Cell 21, 1335-49. Applications: ChIP
- Zhao, J.X. et al. (2011) J Biol Chem 286, 16426-34. Applications: Western Blotting
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know !
Companion Products
- 9753 Di-Methyl-Histone H3 (Lys9) Antibody
- 4069 Pan-Methyl-Histone H3 (Lys9) Antibody
- 9706 Phospho-Histone H3 (Ser10) (6G3) Mouse mAb
- 9701 Phospho-Histone H3 (Ser10) Antibody
- 9675 Acetyl-Histone H3 (Lys18) Antibody
- 9715 Histone H3 Antibody
- 9717 Histone H3 (3H1) Rabbit mAb
- 7071 Phototope® -HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
- 9711 Acetyl- and Phospho-Histone H3 (Lys9/Ser10) Antibody
- 1083 Acetyl-Histone H3 (Lys9) Blocking Peptide
- 9002 SimpleChIP® Enzymatic Chromatin IP Kit (Agarose Beads)
- 9003 SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads)
- 7017 6-Tube Magnetic Separation Rack
For Research Use Only. Not For Use In Diagnostic Procedures.
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文献和实验) AntibodyRabbitW IHC IC9671Acetyl-Histone H3 (Lys9) AntibodyRabbitW IP IHC IC2591Acetyl-Histone H4 (Lys12) AntibodyRabbitW IP IHC IC2594Acetyl-Histone H4 (Lys8) AntibodyRabbitW IP IHC IC有时组蛋白同时乙酰化和磷酸化修饰，进行转录的调控和活性的调节，因此CST也推出了能同时检测乙酰化和磷酸化的组蛋白的抗体。该系列抗体只识别同时乙酰化和磷酸
Competing Chromosomal Proteins from Drosophila Polytene Chromosomes Using Modified Histone Peptides
to chromatin, by its histone tail interaction or by other interactions, then you would not expect to see loss of binding upon peptide competition. A good positive control for the assay is to use H3K9 dimethylated peptide to compete HP1 from the pericentric
INTRODUCTION In cells and tissues, the histone proteins that constitute the nucleosomes can present multiple post-translational modifications, such as lysine acetylation, lysine and arginine methylation, serine phosphorylation
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