- 询价
- Cell Signaling Technology
- 5198
- USA
- 2026年05月28日
- 免疫荧光（IF）,流式细胞（Flow Cyt）
- 小鼠
- 人
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- 文献和实验
- 技术资料
- 抗体英文名：
EpCAM (VU1D9) Mouse mAb (Alexa Fluor 488 Conjugate)
- 抗原：
/
- 应用范围：
免疫荧光（IF）,流式细胞（Flow Cyt）
- 宿主：
小鼠
- 供应商：
CST
- 级别：
详见MSDS文件
- 库存：
大量
- 抗原来源：
/
- 适应物种：
人
- 保质期：
详见说明书
- 是否单克隆：
1
- 保存条件：
-20°c
- 规格：
100 ul (50 tests)/<a href="http://www.cellsignal.com/ddt/custom_reagents.html" target="_blank">carrier free & custom formulation / quantity</a> /100 ul (50 tests)/<a href="http://www.cellsignal.com/ddt/custom_reagents.html" target="_blank">carrier free & custom formulation / quantity</a>
| 规格： | 产品价格： | ￥面议 | |
|---|---|---|---|
| 规格： | 100 ul (50 tests) | 产品价格： | ￥请询价 |
| 规格： | <a href="http://www.cellsignal.com/ddt/custom_reagents.html" target="_blank">carrier free & custom formulation / quantity</a> | 产品价格： | ￥请询价 |
| 规格： | 100 ul (50 tests) | 产品价格： | ￥请询价 |
| 规格： | <a href="http://www.cellsignal.com/ddt/custom_reagents.html" target="_blank">carrier free & custom formulation / quantity</a> | 产品价格： | ￥请询价 |
Product Pathways - Cytoskeletal Signaling
EpCAM (VU1D9) Mouse mAb (Alexa Fluor® 488 Conjugate) #5198
PhosphoSitePlus ® protein, site, and accession data: EPCAM
| Applications | Reactivity | Sensitivity | Isotype |
|---|---|---|---|
| IF-IC F | H | Endogenous | Mouse IgG1 |
Applications Key: IF-IC=Immunofluorescence (Immunocytochemistry) F=Flow Cytometry
Reactivity Key: H=Human
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
- 5198:
- Flow
Specificity / Sensitivity
EpCAM (VU1D9) Mouse mAb (Alexa Fluor® 488 Conjugate) detects endogenous levels of total EpCAM protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with NCI-H69 small cell lung carcinoma cells.
Flow Cytometry
Flow cytometric analysis of unpermeabilized HT-29 cells using EpCAM (VU1D9) Mouse mAb (Alexa Fluor® 488 Conjugate) (green) compared to Mouse (MOPC-21) mAb IgG1 Isotype Control (Alexa Fluor® 488 Conjugate) #4878 (red).
IF-IC
Confocal immunofluorescent analysis of HT-29 cells using EpCAM (VU1D9) Mouse mAb (Alexa Fluor® 488 Conjugate) (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
Description
This Cell Signaling Technology antibody is conjugated to Alexa Fluor® 488 fluorescent dye and tested in-house for direct flow cytometry and immunofluorescent analysis in human cells. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated EpCAM (VU1D9) Mouse mAb #2929.
Background
Epithelial cell adhesion and activating molecule (EpCAM/CD326) is a transmembrane glycoprotein that mediates Ca2+ -independent, homophilic adhesions on the basolateral surface of most epithelial cells. EpCAM is not expressed in adult squamous epithelium, but it is highly expressed in adeno and squamous cell carcinomas (1). One of the first tumor-associated antigens discovered, EpCAM has long been a marker of epithelial and tumor tissue. Because of its high expression in cancer, EpCAM has become a therapeutic target for human solid tumors of epithelial lineage (reviewed in 2,3).
- Went, P.T. et al. (2004) Hum Pathol 35, 122-8.
- Baeuerle, P.A. and Gires, O. (2007) Br J Cancer 96, 417-23.
- Armstrong, A. and Eck, S.L. Cancer Biol Ther 2, 320-6.
Application References
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know !
Companion Products
- 2929 EpCAM (VU1D9) Mouse mAb
- 4878 Mouse (MOPC-21) mAb IgG1 Isotype Control (Alexa Fluor® 488 Conjugate)
- 5447 EpCAM (VU1D9) Mouse mAb (Alexa Fluor® 647 Conjugate)
- 5488 EpCAM (VU1D9) Mouse mAb (Alexa Fluor® 555 Conjugate)
For Research Use Only. Not For Use In Diagnostic Procedures.
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文献和实验：Catalog NumberProduct NameProduct TypeSizeApplication4408SAnti-mouse IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate)Secondary Antibody250µlIF-IC F HCA4409SAnti-mouse IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 555 Conjugate)Secondary
WHOLE MOUNT IN SITU HYBRIDIZATION. Mouse Embryos
4.5 (without removing prehybridization solution.) C. Repeat the addition of the 2XSSC wash twice more. Remove the mix and wash twice, 30 min each time, in 2XSSC pH7/0.1% CHAPS 70 °C. Wash twice, 10 min each, in Maleic Acid Buffer ( MAB; 100 mM maleic acid
：使用 Anti-phospho-Akt (Ser473) Rabbit mAb 对石蜡包埋的人乳腺癌组织进行免疫组织化学分析。（图 A）使用免疫组化试剂盒M&R HRP/DAB Detection IHC Kit，抗体 1:100 稀释；(图 B) 采用普通免疫组化试剂盒，抗体 1:25 稀释。 图 6 免疫组化实验检测 Erk1/2 表达 注：使用 Anti-Erk1/2 Mouse mAb与p44/42 MAPK （Erk1/2）Rabbit mAb 对正常小鼠心脏组织进行免疫
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