- 询价
- Cell Signaling Technology
- 5017
- USA
- 2025年09月08日
- W, F
- All
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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 抗体英文名：
HA-Tag (C29F4) Rabbit mAb (Biotinylated)
- 抗原：
synthetic peptide containing the influenza hemagglutinin epitope (YPYDVPDYA)
- 应用范围：
W, F
- 供应商：
CST
- 库存：
大量
- 级别：
详见MSDS文件
- 保质期：
详见说明书
- 适应物种：
All
- 是否单克隆：
1
- 保存条件：
-20°c
- 规格：
100 ul (10 western blots)/carrier free & custom formulation / quantity
| 规格： | 产品价格： | ￥请询价 | |
|---|---|---|---|
| 规格： | 100 ul (10 western blots) | 产品价格： | ￥请询价 |
| 规格： | carrier free & custom formulation / quantity | 产品价格： | ￥请询价 |
pathway more info application references datasheet PDF MSDS PDF protocols
Applications Key: W=Western Blotting F=Flow Cytometry
Reactivity Key: All=All species expected
Species cross-reactivity is determined by western blot.
| Applications | Reactivity | Sensitivity | Isotype |
|---|---|---|---|
| W F | All | Transfected Only | Rabbit IgG |
| Protocols |
|
|---|---|
| Specificity / Sensitivity | HA-Tag (C29F4) Rabbit mAb (Biotinylated) detects exogenously expressed proteins containing the HA epitope tag. |
| Source / Purification | Monoclonal antibody is produced by immunizing animals with a synthetic peptide containing the influenza hemagglutinin epitope (YPYDVPDYA). Western Blotting
Western blot analysis of extracts from COS cells, untransfected or transfected with a construct overexpressing HA-Akt, using HA-Tag (C29F4) Rabbit mAb (Biotinylated). Detection was performed with Streptavidin-HRP followed by LumiGlo® and Peroxidase #7003. Flow Cytometry
Flow cytometric analysis of COS cells, untransfected (blue) or transfected with HA-Tag (green), using HA-Tag (C29F4) Rabbit mAb (Biotinylated). |
| Description | This Cell Signaling Technology (CST) antibody is conjugated to biotin under optimal conditions and tested using COS cells transfected with HA-tagged protein. |
| Background | Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation and immunostaining techniques. Due to their small size, they are unlikely to affect the tagged protein's biochemical properties. The HA tag is derived from an epitope of the influenza hemagglutinin protein which has been used extensively as a general epitope tag in expression vectors (1). |
| Application References | Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know ! |
| Companion Products |
For Research Use Only. Not For Use In Diagnostic Procedures. |
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文献和实验组织化学分析。（图 C）使用Anti-Erk1/2 Mouse mAb 鼠单抗进行目的蛋白检测；(图 D) 使用p44/42 MAPK （Erk1/2）Rabbit mAb 兔单抗进行目的蛋白检测。 图 7 免疫组化实验检测Akt表达 注：使用 Anti-Akt (pan) Rabbit mAb 对正常小鼠肝组织进行免疫组织化学分析。（图 E）使用免疫组化试剂盒 M&R HRP/DAB Detection IHC Kit，抗体1:100 稀释；(图F)使用另一种试剂盒，抗体 1:100 稀释。
。所以，我们建议使用微波炉或高压锅加热煮沸以达到最佳抗原修复效果。抗体：Phospho–Stat3 (Tyr705)(D3A7) XP. Rabbit mAb #9145样本：石蜡包埋人类肺部肿瘤样本煮沸设备：浴锅 (左)，微波炉 (中)，高压锅 (右)免疫染色—封闭在 IHC–P 中，我们建议在室温下在含有 Tween20 的 TBST 缓冲液和 5% 正常山羊血清 (NGS) 中封闭样品，时长 1 小时，以防止非特异性背景染色。在进行 IHC–F 时，在含有 0.3% Triton. X–100 的 1X
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