Caspase-8 (D35G2) Rabbit mAb产品图

Caspase-8 (D35G2) Rabbit mAb

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  • 询价
  • Cell Signaling Technology已认证
  • USA
  • 2026年05月28日
  • W
  • H,M,R,Mk,Pg
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 抗体英文名:

      Caspase-8 (D35G2) Rabbit mAb

    • 抗原:

      synthetic peptide corresponding to residues near the carboxyl terminus of human caspase-8 protein

    • 应用范围:

      W

    • 库存:

      大量

    • 级别:

      详见MSDS文件

    • 适应物种:

      H,M,R,Mk,Pg

    • 供应商:

      CST

    • 保质期:

      详见说明书

    • 是否单克隆:

      1

    • 保存条件:

      -20°c

    • 规格:

      100 ul (10 western blots)/carrier free & custom formulation / quantity

    规格:产品价格:¥请询价
    规格:100 ul (10 western blots)产品价格:¥请询价
    规格:carrier free & custom formulation / quantity产品价格:¥请询价

    pathway more info application references datasheet PDF MSDS PDF protocols

    Applications Key:  W=Western Blotting
    Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey  Pg=Pig
    Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

    Applications Reactivity Sensitivity MW (kDa) Isotype
    W H M R (Mk) (Pg) Endogenous 10, 57 Rabbit IgG
    Protocols
    Specificity / Sensitivity

    Caspase-8 (D35G2) Rabbit mAb detects endogenous levels of total caspase-8, including the p10 subunit of the activated protein.

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxyl terminus of human caspase-8 protein.

    Western Blotting

    Western Blotting

    Western blot analysis of extracts from HeLa, THP-1, and YB2/0 cell lines using Caspase-8 (D35G2) Rabbit mAb.

    Western Blotting

    Western Blotting

    Western blot analysis of extracts from CTLL-2 cells, untreated or treated with cycloheximide (CHX, 10 μg/ml, overnight) followed by Human Tumor Necrosis Factor-α (hTNF-α) #8902 (20 ng/ml, 4 hours), using Caspase-8 (D35G2) Rabbit mAb.

    Background

    Apoptosis induced through the CD95 receptor (Fas/APO-1) and tumor necrosis factor receptor 1 (TNFR1) activates caspase-8 and leads to the release of the caspase-8 active fragments, p18 and p10 (1-3). Activated caspase-8 cleaves and activates downstream effector caspases such as caspase-1, -3, -6, and -7. Caspase-3 ultimately elicits the morphological hallmarks of apoptosis, including DNA fragmentation and cell shrinkage.

    1. Muzio, M. et al. (1996) Cell 85, 817-827.
    2. Boldin, M.P. et al. (1996) Cell 85, 803-815.
    3. Fernandes-Alnemri, T. et al. (1996) Proc. Natl. Acad. Sci. USA 93, 7464-7469.
    Application References

    Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know !

    Companion Products

    For Research Use Only. Not For Use In Diagnostic Procedures.

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    图标文献和实验
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