- 询价
- Cell Signaling Technology
- 4561
- USA
- 2025年10月13日
- W
- H,M,R
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- 详细信息
- 文献和实验
- 技术资料
- 抗体英文名：
Phospho-HER3/ErbB3 (Tyr1197) (C56E4) Rabbit mAb
- 抗原：
synthetic phosphopeptide corresponding to residues surrounding Tyr1197 of human HER3/ErbB3
- 应用范围：
W
- 级别：
详见MSDS文件
- 供应商：
CST
- 适应物种：
H,M,R
- 库存：
大量
- 保质期：
详见说明书
- 是否单克隆：
1
- 保存条件：
-20°c
- 规格：
100 ul (10 western blots)/carrier free & custom formulation / quantity
| 规格： | 产品价格： | ￥请询价 | |
|---|---|---|---|
| 规格： | 100 ul (10 western blots) | 产品价格： | ￥请询价 |
| 规格： | carrier free & custom formulation / quantity | 产品价格： | ￥请询价 |
pathway more info application references datasheet PDF MSDS PDF protocols
Applications Key: W=Western Blotting
Reactivity Key: H=Human M=Mouse R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W | H (M) (R) | Endogenous | 185 kDa | Rabbit IgG |
| Protocols |
|
|---|---|
| Specificity / Sensitivity | Phospho-HER3/ErbB3 (Tyr1197) (C56E4) Rabbit mAb detects endogenous levels of HER3/ErbB3 protein only when phosphorylated at Tyr1197. The antibody does not cross-react with other phosphorylated receptor tyrosine kinases. |
| Source / Purification | Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr1197 of human HER3/ErbB3. Western Blotting
Western blot analysis of extracts from T47D cells, untreated or treated with neuregulin (NRG), using Phospho-HER3/ErbB3 (Tyr1197) (C56E4) Rabbit mAb (upper) and HER3/ErbB3 (1B2) Rabbit mAb #4754 (lower). |
| Background | HER3/ErbB3 is a member of the ErbB receptor protein tyrosine kinase family, but it lacks tyrosine kinase activity. Tyrosine phosphorylation of ErbB3 depends on its association with other ErbB tyrosine kinases. Upon ligand binding, heterodimers form between ErbB3 and other ErbB proteins, and ErbB3 is phosphorylated on tyrosine residues by the activated ErbB kinase (1,2). There are at least 9 potential tyrosine phosphorylation sites in the carboxy-terminal tail of ErbB3. These sites serve as consensus binding sites for signal transducing proteins, including Src family members, Grb2, and the p85 subunit of PI3 kinase, which mediate ErbB downstream signaling (3). Both Tyr1222 and Tyr1289 of ErbB3 reside within a YXXM motif and participate in signaling to PI3K (4).Investigators have found that ErbB3 is highly expressed in many cancer cells (5) and activation of the ErbB3/PI3K pathway is correlated with malignant phenotypes of adenocarcinomas (6). Research studies have demonstrated that in tumor development, ErbB3 may function as an oncogenic unit together with other ErbB members (e.g. ErbB2 requires ErbB3 to drive breast tumor cell proliferation) (7). Thus, investigators view inhibiting interaction between ErbB3 and ErbB tyrosine kinases as a novel strategy for anti-tumor therapy.
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| Application References | Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know ! |
| Companion Products |
For Research Use Only. Not For Use In Diagnostic Procedures. |
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文献和实验，EDTA 缓冲液适用于多数磷酸化酪氨酸特异性抗体，而柠檬酸盐缓冲液适用于其他多数抗体。每天都需制备新鲜的 1X 溶液。抗体：hospho-HER3/ErbB3 (Tyr1289) (D1B5) Rabbit mAb #2842样本：蜡包埋人类肺部肿瘤抗原修复缓冲液：柠檬酸盐缓冲液（左）或者 EDTA 缓冲液（右）煮沸装置载玻片在所推荐的缓冲液中加热煮沸一段时间后，才可引起抗原修复。该步骤通常在微波炉或高压锅中进行。虽然，一些研究人员也使用水浴锅，但是，因为该步骤中所选择的装置对染色存在积极或消极的影响
h）即可。2. 修复大法——不仅仅是「煮一煮」微波炉修复：简单易行效果好，CST 推荐使用微波炉完成修复。合适的修复液：根据抗体说明书使用合适的修复液。用柠檬酸修复后，切片需浸泡在修复液中，自然冷却；而用 EDTA 修复后，切片可直接从修复缸中取出，直接进行下一步。注：使用不同的修复方式和不同生产商的抗体检测人肺癌组织中 EGFR 的表达。第一排为 CST 的 EGF Receptor (D38B1) XP® Rabbit mAb（#4267），EDTA 的修复方式明显优于柠檬酸盐及胃蛋白
used in accordance withmanufacturer recommendationsSignalStain?DAB Substrate Kit #8059:IHC analysis of paraffin-embedded human breastcarcinoma using Phospho-Stat3 (Tyr705) (D3A7) XP? Rabbit mAb #9145. Chromogenicdetection was performed using #8059 (left
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