PTEN Antibody

价格询价

品牌Cell Signaling Technology
货号
9552
产地USA
更新日期2026年05月28日

应用范围W, IP
宿主Rabbit
适应物种H,M,R,Hm,Mk,C

美国CST中国公司(丁香通商铺)

品牌商

13 年钻石会员

详细信息
文献和实验
技术资料

抗体英文名：
PTEN Antibody
抗原：
synthetic peptide corresponding to the carboxy terminus of human PTEN
应用范围：
W, IP
宿主：
Rabbit
供应商：
CST
级别：
详见MSDS文件
保质期：
详见说明书
库存：
大量
适应物种：
H,M,R,Hm,Mk,C
是否单克隆：
2
保存条件：
-20°c
规格：
100 ul (10 western blots)/carrier free & custom formulation / quantity

规格：		产品价格：	￥请询价
规格：	100 ul (10 western blots)	产品价格：	￥请询价
规格：	carrier free & custom formulation / quantity	产品价格：	￥请询价

pathway more info application references datasheet PDF MSDS PDF protocols

Applications Key: W=Western Blotting IP=Immunoprecipitation
Reactivity Key: H=Human M=Mouse R=Rat Hm=Hamster Mk=Monkey C=Chicken
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Applications	Reactivity	Sensitivity	MW (kDa)	Source
W IP	H M R Hm Mk (C)	Endogenous	54	Rabbit

Protocols	9552: Immunoprecipitation , Western Blotting
Specificity / Sensitivity	PTEN Antibody detects endogenous levels of total PTEN protein. The antibody does not cross-react with related proteins.
Source / Purification	Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the carboxy terminus of human PTEN. Antibodies are purified by protein A and peptide affinity chromatography. Western Blotting Western blot analysis of extracts from HeLa cells, transfected with 100 nM SignalSilence^® Control siRNA (Fluorescein Conjugate) #6201 (-) or SignalSilence^® PTEN siRNA I (+), using PTEN Antibody #9552 and p42 MAPK (Erk2) Antibody #9108. PTEN Antibody confirms silencing of PTEN expression, while the p42 MAPK (Erk2) Antibody is used to control for loading and specificity of PTEN siRNA. Western Blotting Western blot analysis of extracts from HeLa cells, transfected with 100 nM SignalSilence^® Control siRNA (Fluorescein Conjugate) #6201 (-) or SignalSilence^® PTEN siRNA II (+), using PTEN (138G6) Rabbit mAb and β-Actin (13E5) Rabbit mAb #4970. PTEN (138G6) Rabbit mAb confirms silencing of PTEN expression, while the β-Actin (13E5) Rabbit mAb is used to control for loading and specificity of PTEN siRNA Western Blotting Western blot analysis of extracts from various cell lines, using PTEN Antibody.
Background	PTEN (phosphatase and tensin homologue deleted on chromosome ten), also referred to as MMAC (mutated in multiple advanced cancers) phosphatase, is a tumor suppressor implicated in a wide variety of human cancers (1). PTEN encodes a 403 amino acid polypeptide originally described as a dual-specificity protein phosphatase (2). The main substrates of PTEN are inositol phospholipids generated by the activation of the phosphoinositide 3-kinase (PI3K) (3). PTEN is a major negative regulator of the PI3K/Akt signaling pathway (1,4,5). PTEN possesses a carboxy-terminal, noncatalytic regulatory domain with three phosphorylation sites (Ser380, Thr382, and Thr383) that regulate PTEN stability and may affect its biological activity (6,7). PTEN regulates p53 protein levels and activity (8) and is involved in G protein-coupled signaling during chemotaxis (9,10). Cantley, L.C. and Neel, B.G. (1999) Proc Natl Acad Sci USA 96, 4240-5. Myers, M.P. et al. (1997) Proc Natl Acad Sci USA 94, 9052-7. Myers, M.P. et al. (1998) Proc Natl Acad Sci USA 95, 13513-8. Wan, X. and Helman, L.J. (2003) Oncogene 22, 8205-11. Wu, X. et al. (1998) Proc Natl Acad Sci USA 95, 15587-91. Vazquez, F. et al. (2000) Mol Cell Biol 20, 5010-8. Torres, J. and Pulido, R. (2001) J Biol Chem 276, 993-8. Freeman, D.J. et al. (2003) Cancer Cell 3, 117-30. Funamoto, S. et al. (2002) Cell 109, 611-23. Iijima, M. and Devreotes, P. (2002) Cell 109, 599-610.
Application References	Wen, S. et al. (2001) PTEN controls tumor-induced angiogenesis. Proc. Natl. Acad. Sci. USA 98, 4622-4627. Applications: Western Blotting Freeman, D. J. et al. (2003) PTEN tumor suppressor regulates p53 protein levels and activity through phosphatase-dependent and -independent mechanisms. Cancer Cell 3, 117-130. Applications: IP Western Blotting Vaidya, K.S. et al. (2009) Cancer Lett 281, 100-7. Applications: Western Blotting Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know !
Companion Products	9551 Phospho-PTEN (Ser380) Antibody 9554 Phospho-PTEN (Ser380/Thr382/383) Antibody 9556 PTEN (26H9) Mouse mAb 9559 PTEN (138G6) Rabbit mAb 9549 Phospho-PTEN (Ser380/Thr382/383) (44A7) Rabbit mAb 9916 Phospho-Akt Pathway Antibody Sampler Kit 7074 Anti-rabbit IgG, HRP-linked Antibody 7720 Prestained Protein Marker, Broad Range (Premixed Format) 7727 Biotinylated Protein Ladder Detection Pack 7003 20X LumiGLO^® Reagent and 20X Peroxide 9901 LY294002 (PI3 Kinase Inhibitor) For Research Use Only. Not For Use In Diagnostic Procedures.