- 询价
- Cell Signaling Technology
- 4097
- USA
- 2025年09月09日
- IP, F
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- 详细信息
- 技术资料
- 抗体英文名：
Mouse (MOPC-21) mAb IgG1 Isotype Control (Biotinylated)
- 应用范围：
IP, F
- 供应商：
CST
- 保质期：
详见说明书
- 库存：
大量
- 级别：
详见MSDS文件
- 是否单克隆：
0
- 保存条件：
-20°c
- 规格：
100 ul (50 micrograms)/carrier free & custom formulation / quantity
| 规格： | 产品价格： | ￥请询价 | |
|---|---|---|---|
| 规格： | 100 ul (50 micrograms) | 产品价格： | ￥请询价 |
| 规格： | carrier free & custom formulation / quantity | 产品价格： | ￥请询价 |
pathway more info application references datasheet PDF MSDS PDF protocols
Applications Key: IP=Immunoprecipitation F=Flow Cytometry
Reactivity Key:
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| IP F | Endogenous | N/A | Mouse IgG1 |
| Protocols |
|
|---|---|
| Specificity / Sensitivity | Mouse (MOPC-21) mAb IgG1 Isotype Control (Biotinylated) has unknown specificity and was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed rat and human tissues. |
| Source / Purification | Mouse (MOPC-21) mAb IgG1 Isotype Control (Biotinylated) was purified by affinity chromatography. IP
Immunoprecipitation of extracts from Jurkat cells treated with either LY294002 #9901 (1) or Calyculin A #9902 (2), using Akt (pan) (40D4) Mouse mAb (Biotinylated) #4821 and Mouse (MOPC-21) mAb IgG1 Isotype Control (Biotinylated). Immunocomplexes were pulled down using Immobilized Streptavidin (Bead Conjugate) #3419. Western blotting analysis was performed using Akt (pan) (C67E7) Rabbit mAb #4691. IP
Immunoprecipitation of extracts from Jurkat cells treated with either LY294002 #9901 (1) or Calyculin A #9902 (2), using Phospho-Akt (Ser473) (587F11) Mouse mAb (Biotinylated) #5102 and Mouse (MOPC-21) mAb IgG1 Isotype Control (Biotinylated). Immunocomplexes were pulled down using Immobilized Streptavidin (Bead Conjugate) #3419. Western blotting analysis was performed using Phospho-Akt (Ser473) (D9E) Rabbit mAb #4060. Flow Cytometry
Flow cytometric analysis of Jurkat cells using Akt (pan) (40D4) Mouse mAb (Biotinylated) #4821 (blue) compared to Mouse (MOPC-21) mAb IgG1 Isotype Control (Biotinylated) (red). |
| Description | This Cell Signaling Technology monoclonal antibody is conjugated to biotin under optimal conditions. |
| Background | Isotype control antibodies are used to estimate the nonspecific binding of target primary antibodies due to Fc receptor binding or other protein-protein interactions. An isotype control antibody should have the same immunoglobulin type and be used at the same concentration as the test antibody. |
| Application References | Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know ! |
| Companion Products |
For Research Use Only. Not For Use In Diagnostic Procedures. |
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