- 询价
- Cell Signaling Technology
- 4097
- USA
- 2025年09月09日
- IP, F
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- 详细信息
- 文献和实验
- 技术资料
- 抗体英文名：
Mouse (MOPC-21) mAb IgG1 Isotype Control (Biotinylated)
- 应用范围：
IP, F
- 供应商：
CST
- 保质期：
详见说明书
- 库存：
大量
- 级别：
详见MSDS文件
- 是否单克隆：
0
- 保存条件：
-20°c
- 规格：
100 ul (50 micrograms)/carrier free & custom formulation / quantity
| 规格： | 产品价格： | ￥请询价 | |
|---|---|---|---|
| 规格： | 100 ul (50 micrograms) | 产品价格： | ￥请询价 |
| 规格： | carrier free & custom formulation / quantity | 产品价格： | ￥请询价 |
pathway more info application references datasheet PDF MSDS PDF protocols
Applications Key: IP=Immunoprecipitation F=Flow Cytometry
Reactivity Key:
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| IP F | Endogenous | N/A | Mouse IgG1 |
| Protocols |
|
|---|---|
| Specificity / Sensitivity | Mouse (MOPC-21) mAb IgG1 Isotype Control (Biotinylated) has unknown specificity and was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed rat and human tissues. |
| Source / Purification | Mouse (MOPC-21) mAb IgG1 Isotype Control (Biotinylated) was purified by affinity chromatography. IP
Immunoprecipitation of extracts from Jurkat cells treated with either LY294002 #9901 (1) or Calyculin A #9902 (2), using Akt (pan) (40D4) Mouse mAb (Biotinylated) #4821 and Mouse (MOPC-21) mAb IgG1 Isotype Control (Biotinylated). Immunocomplexes were pulled down using Immobilized Streptavidin (Bead Conjugate) #3419. Western blotting analysis was performed using Akt (pan) (C67E7) Rabbit mAb #4691. IP
Immunoprecipitation of extracts from Jurkat cells treated with either LY294002 #9901 (1) or Calyculin A #9902 (2), using Phospho-Akt (Ser473) (587F11) Mouse mAb (Biotinylated) #5102 and Mouse (MOPC-21) mAb IgG1 Isotype Control (Biotinylated). Immunocomplexes were pulled down using Immobilized Streptavidin (Bead Conjugate) #3419. Western blotting analysis was performed using Phospho-Akt (Ser473) (D9E) Rabbit mAb #4060. Flow Cytometry
Flow cytometric analysis of Jurkat cells using Akt (pan) (40D4) Mouse mAb (Biotinylated) #4821 (blue) compared to Mouse (MOPC-21) mAb IgG1 Isotype Control (Biotinylated) (red). |
| Description | This Cell Signaling Technology monoclonal antibody is conjugated to biotin under optimal conditions. |
| Background | Isotype control antibodies are used to estimate the nonspecific binding of target primary antibodies due to Fc receptor binding or other protein-protein interactions. An isotype control antibody should have the same immunoglobulin type and be used at the same concentration as the test antibody. |
| Application References | Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know ! |
| Companion Products |
For Research Use Only. Not For Use In Diagnostic Procedures. |
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文献和实验Rapid Isotyping of Mouse Monoclonal Antibodies
for 5% of mouse antibodies) has limited practical value. In contrast, the heavy-chain isotype is very informative (see Table 1 ); protein A or protein G binding, euglobulin properties, complement binding, kinetics of digestion with papain or pepsin, clearance
Immunofluorescent Staining of Mouse and Rat Leukocytes
mAb. It is important to verify that no secondary reagent will bind the BD Fc Block™. Please see description in "The Uses of BD Fc Block™ in Immunophenotyping of Mouse and Rat Leukocytes". For very low-density cell surface markers (e.g.,cytokine
【翻译】Development trends for monoclonal antibody cancer therapeutics
as IgG2a, four mAbs were IgG3, four were IgG1, and one mAb was known only to be IgG. No correlation was observed between the isotype of the naked murine mAbs and the available information on the primary reason for termination of development (for example
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