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GADD45 alpha (D17E8) Rabbit mA

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  • 询价
  • Cell Signaling Technology已认证
  • USA
  • 2025年10月23日
  • W
  • H,M,R,Mk
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    • 详细信息
    • 询价记录
    • 文献和实验
    • 技术资料
    • 抗体英文名

      GADD45 alpha (D17E8) Rabbit mAb

    • 抗原

      synthetic peptide corresponding to amino acids surrounding Asp129 of human GADD45 alpha

    • 应用范围

      W

    • 供应商

      CST

    • 库存

      大量

    • 适应物种

      H,M,R,Mk

    • 保质期

      详见说明书

    • 级别

      详见MSDS文件

    • 是否单克隆

      1

    • 保存条件

      -20°c

    • 规格

      100 ul (10 western blots)/carrier free & custom formulation / quantity

    规格:产品价格:¥请询价
    规格:100 ul (10 western blots)产品价格:¥请询价
    规格:carrier free & custom formulation / quantity产品价格:¥请询价

    pathway more info application references datasheet PDF MSDS PDF protocols

    Applications Key:  W=Western Blotting
    Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
    Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

    Applications Reactivity Sensitivity MW (kDa) Isotype
    W H M R Mk Endogenous 22 Rabbit IgG
    Protocols
    Specificity / Sensitivity

    GADD45 alpha Antibody detects endogenous levels of total GADD45 alpha protein.

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to amino acids surrounding Asp129 of human GADD45 alpha.

    Western Blotting

    Western Blotting

    Western blot analysis of extracts from various cell lines and tissues using GADD45 alpha (D17E8) Rabbit mAb.

    Background

    GADD45 α, β and γ are an evolutionarily conserved, homologous family of nuclear proteins that function as stress sensors for cellular physiological and environmental damage. GADD45 proteins are required for the activation of the G2/M checkpoint induced by UV radiation or alkylating agents (1). GADD45-induced G2/M checkpoint is regulated through inactivation of Cdc2-cyclin B1 kinase (2). GADD45 forms a complex with p21 and Cdc2, and may serve as core for interaction with other cell cycle regulators (3,4).

    1. Wang, X.W. et al. (1999) Proc Natl Acad Sci USA 96, 3706-11.
    2. Yang, Q. et al. (2000) J Biol Chem 275, 36892-8.
    3. Zhao, H. et al. (2000) Exp Cell Res 258, 92-100.
    4. Liebermann, D.A. and Hoffman, B. (2008) J Mol Signal 3, 15.
    Application References

    Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know !

    Companion Products

    For Research Use Only. Not For Use In Diagnostic Procedures.

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    • 询问日期
    图标文献和实验
    相关实验
    • Flow cytometric determination of leukocyte surface antigens in whole blood

      and add labeled mAb to each aliquot as desired. Make sure that the final mAb concentration is at saturation - you may need to determine this by titration. Do blocked samples as necessary. Incubate 30 minutes on ice. Note that this is for directly

    • 基于PCR技术的染色质沉淀分析

      that was an interspecific hybrid (H) between Mus musculus domesticus (D, paternal genome) and Mus spretus (S, maternal genome). The native chromatin was then immunoprecipitated with rabbit polyclonal antisera to acetylation at lysine 9 of H3 (H3K9Ac) and to dimethylation

    • ELISPOT (Enzyme-linked ImmunoSPOT)

      Mouse Cytokine ELISPOT Set Catalog No. Capture mAb Detection mAb IFN-γ 88-7384 AN-18 R4-6A2 IL-1α 88-7813 ALF-161 Rabbit

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