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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 抗体英文名:
GADD45 alpha (D17E8) Rabbit mAb
- 抗原:
synthetic peptide corresponding to amino acids surrounding Asp129 of human GADD45 alpha
- 应用范围:
W
- 供应商:
CST
- 库存:
大量
- 适应物种:
H,M,R,Mk
- 保质期:
详见说明书
- 级别:
详见MSDS文件
- 是否单克隆:
1
- 保存条件:
-20°c
- 规格:
100 ul (10 western blots)/carrier free & custom formulation / quantity
| 规格: | 产品价格: | ¥请询价 | |
|---|---|---|---|
| 规格: | 100 ul (10 western blots) | 产品价格: | ¥请询价 |
| 规格: | carrier free & custom formulation / quantity | 产品价格: | ¥请询价 |
pathway more info application references datasheet PDF MSDS PDF protocols
Applications Key: W=Western Blotting
Reactivity Key: H=Human M=Mouse R=Rat Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W | H M R Mk | Endogenous | 22 | Rabbit IgG |
| Protocols |
|
|---|---|
| Specificity / Sensitivity | GADD45 alpha Antibody detects endogenous levels of total GADD45 alpha protein. |
| Source / Purification | Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to amino acids surrounding Asp129 of human GADD45 alpha. |
| Background | GADD45 α, β and γ are an evolutionarily conserved, homologous family of nuclear proteins that function as stress sensors for cellular physiological and environmental damage. GADD45 proteins are required for the activation of the G2/M checkpoint induced by UV radiation or alkylating agents (1). GADD45-induced G2/M checkpoint is regulated through inactivation of Cdc2-cyclin B1 kinase (2). GADD45 forms a complex with p21 and Cdc2, and may serve as core for interaction with other cell cycle regulators (3,4). |
| Application References | Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know ! |
| Companion Products |
For Research Use Only. Not For Use In Diagnostic Procedures. |
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- 内容
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文献和实验Flow cytometric determination of leukocyte surface antigens in whole blood
and add labeled mAb to each aliquot as desired. Make sure that the final mAb concentration is at saturation - you may need to determine this by titration. Do blocked samples as necessary. Incubate 30 minutes on ice. Note that this is for directly
that was an interspecific hybrid (H) between Mus musculus domesticus (D, paternal genome) and Mus spretus (S, maternal genome). The native chromatin was then immunoprecipitated with rabbit polyclonal antisera to acetylation at lysine 9 of H3 (H3K9Ac) and to dimethylation
ELISPOT (Enzyme-linked ImmunoSPOT)
Mouse Cytokine ELISPOT Set Catalog No. Capture mAb Detection mAb IFN-γ 88-7384 AN-18 R4-6A2 IL-1α 88-7813 ALF-161 Rabbit
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