- 询价
- Cell Signaling Technology
- 3753
- USA
- 2025年12月24日
- W, IP, IHC-P, IF-IC
- H,M,R,Mk
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- 详细信息
- 文献和实验
- 技术资料
- 抗体英文名：
JunB (C37F9) Rabbit mAb
- 抗原：
synthetic peptide corresponding to residues surrounding Pro169 of human JunB
- 应用范围：
W, IP, IHC-P, IF-IC
- 保质期：
详见说明书
- 供应商：
CST
- 库存：
大量
- 适应物种：
H,M,R,Mk
- 级别：
详见MSDS文件
- 是否单克隆：
1
- 保存条件：
-20°c
- 规格：
100 ul (10 western blots)/carrier free & custom formulation / quantity
| 规格： | 产品价格： | ￥请询价 | |
|---|---|---|---|
| 规格： | 100 ul (10 western blots) | 产品价格： | ￥请询价 |
| 规格： | carrier free & custom formulation / quantity | 产品价格： | ￥请询价 |
pathway more info application references datasheet PDF MSDS PDF protocols
Applications Key: W=Western Blotting IP=Immunoprecipitation IHC-P=Immunohistochemistry (Paraffin) IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key: H=Human M=Mouse R=Rat Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W IP IHC-P IF-IC | H M R Mk | Endogenous | 42, 43 | Rabbit IgG |
| Protocols | |
|---|---|
| Specificity / Sensitivity | JunB (C37F9) Rabbit mAb detects endogenous levels of total JunB protein. |
| Source / Purification | Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro169 of human JunB. Western Blotting
Western blot analysis of extracts from various cell lines using JunB (C37F9) Rabbit mAb. IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human lung carcinoma using JunB (C37F9) Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right). IF-IC
Confocal immunofluorescent analysis of MCF-7 cells using JunB (C37F9) Rabbit mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red). |
| Background | JunB is a basic region, leucine zipper (bZIP) transcription factor belonging to the Jun family that includes c-Jun and JunD. Jun family members homodimerize or heterodimerize with Fos and ATF proteins to form a functional transcription factor AP-1 (activator protein 1), whose activity is regulated by a variety of physiological and pathological stimuli such as growth factors, infections, and stress signals (1-4). While JunB sometimes antagonizes c-Jun transcriptional activity, it may functionally substitute for c-Jun during development in mice (5-7). JunB regulates hematopoietic stem cell number and plays an important role in the pathogenesis of chronic myelogenous leukemia (CML) and acute myeloid leukemia (AML) (8,9).
|
| Application References | Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know ! |
| Companion Products |
For Research Use Only. Not For Use In Diagnostic Procedures. |
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文献和实验amnion and attached to the visceral yolk sac are dissected from maternal tissues and placed in culture for up to 48 h at approximately 37�C and are continuously exposed to an humidified gas atmosphere mixture in a rotating culture system. During this 48 h
T-Cell Activation Using mAb to CD3
Concanavalin A, optional (ConA, Sigma Cat. No. C5275) Instruments Pipettes and pipettors, Multichannel pipettor Centrifuge 37°C, CO2 incubator 96-well micro test spectrophotometer Experiment Duration
Testing Rabbit Bleeds By Elisa
as a solid if soluble. Mix, adding glutaraldehyde last. Peptide and BSA turn a little yellow even before adding glutaraldehyde. Incubate 90'' at 37 deg C. Add 0.1 volumes of 0.1 M NaBH4 in 0.1 M NaHCO3. There will be some bubbling. Add
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