- 询价
- Cell Signaling Technology
- 3570
- USA
- 2025年07月16日
- western blot,免疫沉淀（IP）,免疫组化（IHC）,免疫荧光（IF）,流式细胞（Flow Cyt）
- 小鼠
- 人
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- 详细信息
- 文献和实验
- 技术资料
- 抗体英文名：
CD44 (156-3C11) Mouse mAb
- 抗原：
/
- 应用范围：
western blot,免疫沉淀（IP）,免疫组化（IHC）,免疫荧光（IF）,流式细胞（Flow Cyt）
- 宿主：
小鼠
- 级别：
详见MSDS文件
- 供应商：
CST
- 抗原来源：
/
- 保质期：
详见说明书
- 适应物种：
人
- 库存：
大量
- 是否单克隆：
1
- 保存条件：
-20°c
- 规格：
100 ul (10 western blots)/<a href="http://www.cellsignal.com/ddt/custom_reagents.html" target="_blank">carrier free & custom formulation / quantity</a> /100 ul (10 western blots)/<a href="http://www.cellsignal.com/ddt/custom_reagents.html" target="_blank">carrier free & custom formulation / quantity</a>
| 规格： | 产品价格： | ￥面议 | |
|---|---|---|---|
| 规格： | 100 ul (10 western blots) | 产品价格： | ￥请询价 |
| 规格： | <a href="http://www.cellsignal.com/ddt/custom_reagents.html" target="_blank">carrier free & custom formulation / quantity</a> | 产品价格： | ￥请询价 |
| 规格： | 100 ul (10 western blots) | 产品价格： | ￥请询价 |
| 规格： | <a href="http://www.cellsignal.com/ddt/custom_reagents.html" target="_blank">carrier free & custom formulation / quantity</a> | 产品价格： | ￥请询价 |
Product Pathways - Lymphocyte Signaling
CD44 (156-3C11) Mouse mAb #3570
PhosphoSitePlus ® protein, site, and accession data: CD44
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W IP IHC-P IF-IC F | H | Endogenous | 80 | Mouse IgG2a |
Applications Key: W=Western Blotting IP=Immunoprecipitation IHC-P=Immunohistochemistry (Paraffin) IF-IC=Immunofluorescence (Immunocytochemistry) F=Flow Cytometry
Reactivity Key: H=Human
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
- 3570:
- Flow , IHC / Paraffin , Immunofluorescence , Immunoprecipitation , Western Blotting
Specificity / Sensitivity
CD44 (156-3C11) Mouse mAb detects endogenous levels of total CD44 protein.
Source / Purification
Monoclonal antibody is produced by immunizing BALB/c mice with stimulated human leukocytes.
Western Blotting
Western blot analysis of extracts from HeLa and PANC1 cell lines using CD44 (156-3C11) Mouse mAb.
IHC-P (paraffin)
Immunohistochemical analysis of human Non-Hodgkin's lymphoma using CD44 (156-3C11) Mouse mAb.
IHC-P (paraffin)
Immunohistochemical analysis of human tonsil using CD44 (156-3C11) Mouse mAb.
Flow Cytometry
Flow cytometric analysis of Hela cells using CD44 (156-3C11) Mouse mAb (blue) compared to a nonspecific negative control antibody (red).
Flow Cytometry
Flow cytometric analysis of whole blood using CD44 (156-3C11) Mouse mAb.
IF-IC
Confocal immunofluorescent analysis of Hela cells using CD44 (156-3C11) Mouse mAb (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
Background
CD44 is a type I transmembrane glycoprotein that mediates cell-cell and cell-matrix interaction through its affinity for hyaluronic acid (HA) and possibly through other parts of the extracellular matrix (ECM). CD44 is highly polymorphic, possesses a number of alternative splice variants and undergoes extensive post-translational modifications (1,2). Increased surface levels of CD44 are characteristic of T cell activation, and expression of the protein is upregulated during the inflammatory response. Interactions between CD44 and HER2 have been linked to an increase in ovarian carcinoma cell growth (1-3). CD44 interacts with ezrin, radixin and moesin (ERM), linking the actin cytoskeleton to the plasma membrane and the ECM (4-6). CD44 is constitutively phosphorylated at Ser325 in resting cells. Activation of PKC results in phosphorylation of Ser291, dephosphorylation of Ser325, disassociation of ezrin from CD44, and directional motility (4).
- Goodison, S. et al. (1999) Mol. Pathol. 52, 189-196.
- Cichy, J. and Puré, E. (2003) J. Cell Biol. 161, 839-843.
- Bourguignon, L.Y. et al. (1997) J. Biol. Chem. 272, 27913-27918.
- Legg, J.W. et al. (2002) Nat. Cell Biol. 4, 399-407.
- Yonemura, S. et al. (1998) J. Cell Biol. 140, 885-895.
- Tsukita, S. et al. (1994) J. Cell Biol. 126, 391-401.
Application References
- Gilbert, C.M. and Parwani, A. (2010) J Pathol Inform 1, 23. Applications: IHC-P (paraffin)
- Golubkov, V.S. et al. (2010) J Biol Chem 285, 35740-9. Applications: Western Blotting
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know !
Companion Products
- 3516 CD44 (156-3C11) Mouse mAb (Alexa Fluor® 488 Conjugate)
- 3578 CD44 Antibody
- 8112 SignalStain® Antibody Diluent
- 4443 CD3ε (CD3-12) Rat mAb
- 3563 CD4 (Edu-2) Mouse mAb
- 3572 CD8 (RIV11) Mouse mAb
- 3565 CD10 (CB-CALLA) Mouse mAb
- 3569 CD34 (ICO115) Mouse mAb
- 3576 CD56 (NCAM) (123C3) Mouse mAb
- 3141 Phospho-Ezrin (Thr567)/Radixin (Thr564)/Moesin (Thr558) Antibody
- 3142 Ezrin/Radixin/Moesin Antibody
- 9921 Phospho-PKC Antibody Sampler Kit
- 9923 Phospho-HER2/ErbB2 Antibody Sampler Kit
- 7072 Phototope® -HRP Western Blot Detection System, Anti-mouse IgG, HRP-linked Antibody
- 7076 Anti-mouse IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
- 8125 SignalStain® Boost IHC Detection Reagent (HRP, Mouse)
For Research Use Only. Not For Use In Diagnostic Procedures.
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文献和实验(adjust pH to 7.8 with Binding buffer; red color) to the Protein A column.Mouse antibodies of the IgG1 subclass do not have a high affinity for protein A. Purification on protein A beads using standard conditions will yield approximately 1/10
T-Cell Activation Using mAb to CD3
splenic T-cells and human peripheral T cells stimulated via CD3. Critical parameters include cell density, antibody titer and activation kinetics. Materials 1X sterile PBS Anti-mouse CD3e, Clone 145-2C11 (Functional Grade, Cat. No. 16
Purification of mAb (IgG) by Chang-Duk Jun, 03/14/2000 Purpose Materials Antibody 7E3 , 2L sup grown in flasks, frozen and thawed overnight. BioRad Affi-Gel Protein A MAPS II Buffers
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