- 询价
- Cell Signaling Technology
- 3344
- USA
- 2025年08月16日
- W, IP, IHC-P
- H,M,R,Mk,Dg,Pg
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- 详细信息
- 文献和实验
- 技术资料
- 抗体英文名：
Jak1 (6G4) Rabbit mAb
- 抗原：
synthetic peptide corresponding to residues surrounding Ile800 of Jak1
- 应用范围：
W, IP, IHC-P
- 适应物种：
H,M,R,Mk,Dg,Pg
- 库存：
大量
- 供应商：
CST
- 保质期：
详见说明书
- 级别：
详见MSDS文件
- 是否单克隆：
1
- 保存条件：
-20°c
- 规格：
40 ul (4 western blots)/100 ul (10 western blots)/carrier free & custom formulation / quantity
| 规格： | 产品价格： | ￥请询价 | |
|---|---|---|---|
| 规格： | 40 ul (4 western blots) | 产品价格： | ￥请询价 |
| 规格： | 100 ul (10 western blots) | 产品价格： | ￥请询价 |
| 规格： | carrier free & custom formulation / quantity | 产品价格： | ￥请询价 |
pathway more info application references datasheet PDF MSDS PDF protocols
Applications Key: W=Western Blotting IP=Immunoprecipitation IHC-P=Immunohistochemistry (Paraffin)
Reactivity Key: H=Human M=Mouse R=Rat Mk=Monkey Dg=Dog Pg=Pig
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W IP IHC-P | H M R (Mk) (Dg) (Pg) | Endogenous | 130 | Rabbit IgG |
| Protocols | |
|---|---|
| Specificity / Sensitivity | Jak1 (6G4) Rabbit mAb detects endogenous levels of total Jak1 protein. It does not cross react with other Jak family members. |
| Source / Purification | Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ile800 of Jak1. Western Blotting
Western blot analysis of extracts from ACHN, SR, CTLL-2 and PC12 cell lines using Jak1 (6G4) Rabbit mAb. IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using Jak1 (6G4) Rabbit mAb #3344, in the presence of control peptide (left) or Jak1 Blocking Peptide (right). IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using Jak1 (6G4) Rabbit mAb. IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human lung carcinoma using Jak1 (6G4) Rabbit mAb. |
| Background | Members of the Janus family of tyrosine kinases (Jak1, Jak2, Jak3, and Tyk2) are activated by ligands binding to a number of associated cytokine receptors (1). Upon cytokine receptor activation, Jak proteins become autophosphorylated and phosphorylate their associated receptors to provide multiple binding sites for signaling proteins. These associated signaling proteins, such as Stats (2), Shc (3), insulin receptor substrates (4), and focal adhesion kinase (FAK) (5), typically contain SH2 or other phospho-tyrosine-binding domains. |
| Application References |
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know ! |
| Companion Products |
Rabbit Monoclonals Produced Using Epitomics® Technology, U.S. Patent No. 5,675,063.Jak antibodies produced under license (granting certain rights including those under U. S. Patent No. 5,658,791) from Chemicon International, Inc. For Research Use Only. Not For Use In Diagnostic Procedures. |
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文献和实验：使用 Anti-phospho-Akt (Ser473) Rabbit mAb 对石蜡包埋的人乳腺癌组织进行免疫组织化学分析。（图 A）使用免疫组化试剂盒M&R HRP/DAB Detection IHC Kit，抗体 1:100 稀释；(图 B) 采用普通免疫组化试剂盒，抗体 1:25 稀释。 图 6 免疫组化实验检测 Erk1/2 表达 注：使用 Anti-Erk1/2 Mouse mAb与p44/42 MAPK （Erk1/2）Rabbit mAb 对正常小鼠心脏组织进行免疫
3A7)XP® Rabbit mAb#9145 检测 p-Stat3 的表达，使用不同的产品进行显色。与竞争者的 DAB 产生的信号相比，SignalStain® DABSubstrateKit 的显色强度更佳。CST 对实验细节进行了步步优化，抗体说明书也是为实验成功保驾护航的必备指南：注：使用 PLK1(208 G4)Rabbit mAb #4513 检测人结肠肿瘤中 PLK1 的表达，在经过辅助试剂的步步优化后发现，使用一抗稀释液#8112 稀释抗体，聚合物型二抗#8114 进行检测，DAB 显色
到 PVDF 膜上，同时减少蛋白不必要降解，这对于最终获得清晰、可信结果也是需要考虑因素。抗体信息：1.ACC1, Recombinant Rabbit monoclonal IgG. HuaAn. HuaAn biotechnology , inc.2.ATGL, Mouse mAb IgG1, Cat#:RT1058. HuaAn biotechnology , inc.3.p-PERK(Thr981), Rabbit Polyclonal IgG primary antibodies, Cat
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