- 询价
- Cell Signaling Technology
- 2955
- USA
- 2025年11月18日
- W, IHC-P, F
- All
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- 详细信息
- 技术资料
- 抗体英文名：
GFP (4B10) Mouse mAb
- 抗原：
full-length recombinant GFP
- 应用范围：
W, IHC-P, F
- 库存：
大量
- 保质期：
详见说明书
- 适应物种：
All
- 供应商：
CST
- 级别：
详见MSDS文件
- 是否单克隆：
1
- 保存条件：
-20°c
- 规格：
100 ul (10 western blots)/carrier free & custom formulation / quantity
| 规格： | 产品价格： | ￥请询价 | |
|---|---|---|---|
| 规格： | 100 ul (10 western blots) | 产品价格： | ￥请询价 |
| 规格： | carrier free & custom formulation / quantity | 产品价格： | ￥请询价 |
pathway more info application references datasheet PDF MSDS PDF protocols
Applications Key: W=Western Blotting IHC-P=Immunohistochemistry (Paraffin) F=Flow Cytometry
Reactivity Key: All=All species expected
Species cross-reactivity is determined by western blot.
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W IHC-P F | All | Transfected Only | 27 | Mouse IgG2a |
| Protocols |
|
|---|---|
| Specificity / Sensitivity | GFP (4B10) Mouse mAb detects GFP-tagged proteins exogenously expressed in cells. Please note that the GFP tag adds approximately 27 kDa to the molecular weight of the fusion protein. |
| Source / Purification | Monoclonal antibody is produced by immunizing animals with full-length recombinant GFP. Western Blotting
Western blot analysis of extracts from HCC827 cells, untransfected or transfected with GFP, using GFP (4B10) Mouse mAb. IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded HCC827 cells, untransfected (left) or transfected with GFP (right) using GFP (4B10) Mouse mAb. Flow Cytometry
Flow cytometric analysis of HCC827 cells, untransfected (blue) or transfected with GFP (green), using GFP (4B10) Mouse mAb. |
| Background | Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein's biochemical properties. The green-fluorescent protein (GFP) functions as a bioluminescence energy transfer acceptor in the jellyfish Aequorea that maximally absorbs light at 395 nm and has an emission spectrum that peaks at 509 nm. GFP has become a very useful tool as a fusion protein that reports gene expression, traces cell lineages and defines subcellular protein localizations (1). |
| Application References | Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know ! |
| Companion Products |
For Research Use Only. Not For Use In Diagnostic Procedures. |
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